pLV3-CMV-TRIM11-3×FLAG-CopGFP-

pLV3-CMV-TRIM11-3×FLAG-CopGFP-Puro plasmid

PVT106893

pLV3-CMV-TRIM11-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TRIM11(human)-3×FLAG-CopGFP-Puro
Alias:
Gene lenght: 1404
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TRIM11-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TRIM11-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-TRIM11-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg ccgcccccga cctgtccacc 2341 aacctccagg aggaggccac ctgcgccatc tgcctcgact acttcacgga tccggtgatg 2401 accgactgcg gccacaactt ctgccgcgag tgcatccggc gctgctgggg ccagcccgag 2461 ggcccgtacg cgtgccccga gtgccgcgag ctgtccccgc agaggaacct gcggcccaac 2521 cgcccgcttg ctaagatggc cgagatggcg cggcgcctgc acccgccgtc gccggtcccg 2581 cagggcgtgt gccccgcgca ccgcgagcca ctggccgcct tctgtggcga cgagctgcgc 2641 ctcctgtgtg cggcctgcga gcgctctggg gagcactggg cgcaccgcgt gcggccgctg 2701 caggacgcgg ccgaagacct caaggcgaag ctggagaagt cactggagca tctccggaag 2761 cagatgcagg atgcgttgct gttccaagcc caggcggatg agacctgcgt cttgtggcag 2821 aagatggtgg agagccagcg gcagaacgtg ctgggtgagt tcgagcgtct tcgccgtttg 2881 ctggcagagg aggagcagca gctgctgcag aggctggagg aggaggagct ggaggtgctg 2941 ccccggctgc gggagggcgc agcccaccta ggccagcaga gcgcccacct agctgagctc 3001 atcgccgagc tcgagggccg ctgccagctg cctgctctgg ggctgctgca ggacatcaag 3061 gacgccctgc gcagggtcca ggatgtgaag ctgcagcccc cagaagttgt gcctatggag 3121 ctgaggaccg tgtgcagggt cccgggactg gtagagacac tgcggaggtt tcgaggggac 3181 gtgaccttgg acccggacac cgccaaccct gagctgatcc tgtctgaaga caggcggagc 3241 gtgcagcggg gggacctacg gcaggccctg ccggacagcc cagagcgctt tgaccccggc 3301 ccctgcgtgc tgggccagga gcgcttcacc tcaggccgcc actactggga ggtggaggtt 3361 ggggaccgca ccagctgggc cctgggggtg tgcagggaga acgtgaacag gaaggagaag 3421 ggcgagctgt ccgcgggcaa cggcttctgg atcctggtct tcctggggag ctattacaat 3481 tcctcggaac gggccttggc tccactccgg gacccaccca ggcgcgtggg gatctttctg 3541 gactacgagg ctggacatct ctctttctac agtgccaccg atgggtcact gctattcatc 3601 tttcccgaga tccccttctc ggggacgctg cggcccctct tctcacccct gtccagcagc 3661 ccgaccccga tgactatctg ccggccgaaa ggtgggtccg gggacaccct ggctccccag 3721 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 3781 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 3841 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 3901 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 3961 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 4021 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 4081 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 4141 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 4201 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 4261 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 4321 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 4381 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 4441 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 4501 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 4561 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 4621 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 4681 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 4741 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 4801 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 4861 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 4921 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 4981 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 5041 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 5101 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 5161 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 5221 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 5281 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 5341 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 5401 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 5461 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 5521 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 5581 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 5641 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 5701 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 5761 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5821 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 5881 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 5941 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 6001 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 6061 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 6121 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 6181 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 6241 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 6301 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 6361 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 6421 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 6481 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 6541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 6601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 6661 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 6721 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 6781 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 6841 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 6901 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6961 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 7021 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 7081 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 7141 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 7201 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 7261 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 7321 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7381 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7441 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7501 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7561 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7621 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7681 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7741 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7801 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7861 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7921 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7981 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 8041 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 8101 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 8161 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 8221 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 8281 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 8341 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 8401 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 8461 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 8521 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 8581 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 8641 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 8701 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 8761 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 8821 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 8881 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 8941 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 9001 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 9061 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 9121 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 9181 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 9241 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 9301 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 9361 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 9421 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 9481 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 9541 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 9601 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 9661 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.