pCMV-HaloTag-GGSX6-AARS1-FLAG-

pCMV-HaloTag-GGSX6-AARS1-FLAG-Neo plasmid

PVT106814

pCMV-HaloTag-GGSX6-AARS1-FLAG-Neo plasmid Information

Plasmid name: pCMV-HaloTag-GGSX6-AARS1(human)-FLAG-Neo
Alias: NM_001605.3;AARS; CMT2N; DEE29; EIEE29; HDLS2; TTD8;
Gene lenght: 2904
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pCMV-HaloTag-GGSX6-AARS1-FLAG-Neo plasmid Description

pCMV-HaloTag-GGSX6-AARS1-FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-HaloTag-GGSX6-AARS1-FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTatgg gcagcgaaat tggcacaggc ttcccgttcg acccgcatta 961 cgttgaggtg ctgggcgagc gcatgcacta tgtggacgtg ggtccgcgtg acggtacccc 1021 tgtgctgttc ctgcatggta acccgaccag cagctacgtg tggcgcaaca ttatcccgca 1081 cgtggccccg GGATCCGGCG GTtctGGAGG CtccGGCGGT tcaGGGGGAt cgGGCGGCtc 1141 aGGCGGCtct GAATTCatgg actctactct aacagcaagt gaaatccggc agcgatttat 1201 agatttcttc aagaggaacg agcatacgta tgttcactcg tctgccacca tcccattgga 1261 tgaccccact ttgctctttg ccaatgcagg catgaaccag tttaaaccca ttttcctgaa 1321 cacaattgac ccatctcacc ccatggcaaa gctgagcaga gctgccaata cccagaagtg 1381 catccgggct gggggcaaac ataatgacct ggacgatgtg ggcaaggatg tctatcatca 1441 caccttcttc gagatgctgg gctcttggtc ttttggagat tactttaagg aattggcatg 1501 taagatggct ctggaactcc tcacccaaga gtttggcatt cccattgaaa gactttatgt 1561 tacttacttt ggcggggatg aagcagctgg cttagaagca gatctggaat gcaaacagat 1621 ctggcaaaat ttggggctgg atgacaccaa aatcctccca ggcaacatga aggataactt 1681 ctgggagatg ggtgacacgg gcccctgtgg tccttgcagt gagatccact acgaccggat 1741 tggtggtcgg gacgccgcac atcttgtcaa ccaggacgac cctaatgtgc tggagatctg 1801 gaaccttgtg ttcatccagt ataacaggga agctgatggc attctgaaac ctcttcccaa 1861 gaaaagcatt gacacaggga tgggcctgga acgactggta tctgtgctgc agaataagat 1921 gtccaactat gacactgacc tttttgtccc ttactttgaa gccattcaga agggcacagg 1981 tgcccgacca tacactggga aagttggtgc tgaggatgcc gatgggattg acatggccta 2041 ccgggtgctg gctgaccacg ctcggaccat cactgtggca ctggctgatg gtggccggcc 2101 tgacaacaca gggcgtggat atgtgttgag acggattctc cgccgagctg tccgatacgc 2161 ccatgaaaag ctcaatgcca gcaggggctt ctttgctacg ttagtggatg ttgtcgtcca 2221 gtccctggga gatgcatttc ctgagctgaa gaaggaccca gacatggtga aggacatcat 2281 taatgaagaa gaggtgcagt ttctcaagac tctcagcaga gggcgtcgca tcctggacag 2341 gaaaattcag agcctgggag acagcaagac cattcccgga gacactgctt ggctcctcta 2401 tgacacctat gggtttccag tggatctgac tggactgatt gctgaagaga agggcctggt 2461 ggtagacatg gatggctttg aagaggagag gaaactggcc cagctgaaat cacagggcaa 2521 gggagctggt ggggaagacc tcattatgct ggacatttac gctatcgaag agctccgggc 2581 acggggtctg gaggtcacag atgattcccc aaagtacaat taccatttgg actccagtgg 2641 tagctatgta tttgagaaca cagtggctac ggtgatggct ctgcgcaggg agaagatgtt 2701 cgtggaagag gtgtccacag gccaggagtg tggagtggtg ctggacaaga cctgtttcta 2761 tgctgagcaa ggaggccaga tctatgacga aggctacctg gtgaaggtgg atgacagcag 2821 tgaagataaa acagagttta cagtgaagaa tgctcaggtc cgaggagggt atgtgctaca 2881 cattggaacc atctacggtg acctgaaagt gggggatcag gtctggctgt ttattgatga 2941 gccccgacga agacccatca tgagcaacca cacagctacg cacattctga acttcgccct 3001 gcgctcagtg cttggggaag ctgaccagaa aggctcattg gttgctcctg accgcctcag 3061 atttgacttt actgccaagg gagccatgtc cacccaacag atcaagaagg ctgaagagat 3121 tgctaatgag atgattgagg cagccaaggc cgtctatacc caggattgcc ccctggcagc 3181 agcgaaagcc atccagggcc tacgggctgt gtttgatgag acctatcctg accctgtgcg 3241 agtcgtctcc attggggtcc cggtgtccga gttgctggat gacccctctg ggcctgctgg 3301 ctccctgact tctgttgagt tctgtggggg aacgcacctg cggaactcga gtcatgcagg 3361 agcttttgtg atcgtgacgg aagaagccat tgccaagggt atccggagga ttgtggctgt 3421 cacaggtgcc gaggcccaga aggccctcag gaaagcagag agcttgaaga aatgtctctc 3481 tgtcatggaa gccaaagtga aggctcagac tgctccaaac aaggatgtgc agagggagat 3541 cgctgacctt ggagaggccc tggccactgc agtcatcccc cagtggcaga aggatgaatt 3601 gcgggagact ctcaaatccc taaagaaggt catggatgac ttggaccgag ccagcaaagc 3661 cgatgtccag aaacgagtgt tagagaagac gaagcagttc atcgacagca accccaacca 3721 gcctcttgtc atcctggaga tggagagcgg cgcctcagcc aaggccctga atgaagcctt 3781 gaagctcttc aagatgcact cccctcagac ttctgccatg ctcttcacgg tggacaatga 3841 ggctggcaag atcacgtgcc tgtgtcaagt tccccagaat gcagccaatc ggggcttaaa 3901 agccagcgag tgggtgcagc aggtgtcagg cttgatggac ggtaaaggtg gtggcaagga 3961 tgtgtctgca caggccacag gcaagaacgt tggctgcctg caggaggcgc tgcagctggc 4021 cacttccttc gcccagctgc gcctcgggga tgtaaagaac GATTACAAGG ATGACGACGA 4081 TAAGTGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC 4141 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 4201 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 4261 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 4321 TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG 4381 GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 4441 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 4501 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 4561 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 4621 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 4681 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 4741 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 4801 AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 4861 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 4921 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 4981 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 5041 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT 5101 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 5161 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 5221 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 5281 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 5341 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 5401 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 5461 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 5521 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 5581 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 5641 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 5701 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 5761 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 5821 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 5881 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 5941 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 6001 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 6061 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 6121 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 6181 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 6241 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 6301 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 6361 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 6421 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 6481 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 6541 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 6601 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 6661 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 6721 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 6781 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 6841 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 6901 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 6961 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 7021 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 7081 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 7141 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 7201 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 7261 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 7321 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 7381 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 7441 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 7501 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 7561 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 7621 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 7681 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 7741 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 7801 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 7861 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 7921 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 7981 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 8041 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 8101 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 8161 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 8221 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 8281 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 8341 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 8401 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 8461 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.