pCMV-3×HA-TRAPPC5-Neo plasmid

pCMV-3×HA-TRAPPC5-Neo plasmid

PVT106664

pCMV-3×HA-TRAPPC5-Neo plasmid Information

Plasmid name: pCMV-3×HA-TRAPPC5(human)-Neo
Alias: NM_174894.3;TRS31
Gene lenght: 567
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×HA-TRAPPC5-Neo plasmid Description

pCMV-3×HA-TRAPPC5-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×HA-TRAPPC5-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGTAC CCATACGACG TACCTGATTA TGCAACTTAT 961 CCTTATGATG TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCATCAGCG 1021 GAAAAGCTTa tggaggcgcg cttcacgcgc gggaagtcgg cgctgctgga gcgcgcgctg 1081 gcgcggccgc gcaccgaggt gagcctgagc gccttcgcac tgctgttctc cgagctggta 1141 cagcactgcc agagccgcgt cttctccgtg gccgagctgc agtcgcgcct ggccgcgctg 1201 ggccgccagg tgggcgcgcg cgtgctggat gcgctggtgg cgcgcgaaaa gggtgcccgg 1261 cgtgagacca aggtgctagg cgcgttgctc ttcgtcaagg gcgccgtgtg gaaggcgctc 1321 ttcggcaagg aggcggacaa gctggagcag gccaacgatg acgcgcgcac cttctacatc 1381 atcgagcgcg agccgctcat caacacctac atctccgtgc ccaaggagaa cagcacgctc 1441 aactgcgcca gcttcacggc gggcatcgtg gaggcggtgc tcacacacag cggcttccct 1501 gccaaggtca cggcgcactg gcacaagggc accacgctca tgatcaagtt cgaggaggca 1561 gtcatcgctc gagaccgggc cctggagggc cgctgagaat tctgcagata tccagcacag 1621 tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct 1681 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 1741 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 1801 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 1861 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 1921 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 1981 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 2041 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 2101 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 2161 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 2221 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 2281 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 2341 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 2401 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2461 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2521 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2581 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2641 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2701 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 2761 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 2821 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 2881 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 2941 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 3001 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 3061 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 3121 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 3181 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 3241 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 3301 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 3361 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 3421 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 3481 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 3541 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 3601 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 3661 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 3721 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 3781 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 3841 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 3901 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 3961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 4021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 4081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 4141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 4201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 4261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 4321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 4741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 4801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggttt 4861 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 4921 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 4981 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 5041 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 5101 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 5161 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 5221 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 5281 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 5341 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 5401 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 5461 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 5521 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 5581 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 5641 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 5701 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 5761 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 5821 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 5881 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 5941 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 6001 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 6061 gccacctgac gtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.