pLV3-CMV-CAMK2G-3×HA-CopGFP-Pu

pLV3-CMV-CAMK2G-3×HA-CopGFP-Puro plasmid

PVT106617

pLV3-CMV-CAMK2G-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CAMK2G(human)-3×HA-CopGFP-Puro
Alias: NM_172171.3;CAMK; CAMK-II; CAMKG; MRD59
Gene lenght: 1668
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CAMK2G-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-CAMK2G-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-CAMK2G-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ccaccaccgc cacctgcacc 2341 cgtttcaccg acgactacca gctcttcgag gagcttggca agggtgcttt ctctgtggtc 2401 cgcaggtgtg tgaagaaaac ctccacgcag gagtacgcag caaaaatcat caataccaag 2461 aagttgtctg cccgggatca ccagaaacta gaacgtgagg ctcggatatg tcgacttctg 2521 aaacatccaa acatcgtgcg cctccatgac agtatttctg aagaagggtt tcactacctc 2581 gtgtttgacc ttgttaccgg cggggagctg tttgaagaca ttgtggccag agagtactac 2641 agtgaagcag atgccagcca ctgtatacat cagattctgg agagtgttaa ccacatccac 2701 cagcatgaca tcgtccacag ggacctgaag cctgagaacc tgctgctggc gagtaaatgc 2761 aagggtgccg ccgtcaagct ggctgatttt ggcctagcca tcgaagtaca gggagagcag 2821 caggcttggt ttggttttgc tggcacccca ggttacttgt cccctgaggt cttgaggaaa 2881 gatccctatg gaaaacctgt ggatatctgg gcctgcgggg tcatcctgta tatcctcctg 2941 gtgggctatc ctcccttctg ggatgaggat cagcacaagc tgtatcagca gatcaaggct 3001 ggagcctatg atttcccatc accagaatgg gacacggtaa ctcctgaagc caagaacttg 3061 atcaaccaga tgctgaccat aaacccagca aagcgcatca cggctgacca ggctctcaag 3121 cacccgtggg tctgtcaacg atccacggtg gcatccatga tgcatcgtca ggagactgtg 3181 gagtgtttgc gcaagttcaa tgcccggaga aaactgaagg gtgccatcct cacgaccatg 3241 cttgtctcca ggaacttctc agctgccaaa agcctattga acaagaagtc ggatggcggt 3301 gtcaagccac agagcaacaa caaaaacagt ctcgtaagcc cagcccaaga gcccgcgccc 3361 ttgcagacgg ccatggagcc acaaaccact gtggtacaca acgctacaga tgggatcaag 3421 ggctccacag agagctgcaa caccaccaca gaagatgagg acctcaaagc tgccccgctc 3481 cgcactggga atggcagctc ggtgcctgaa ggacggagct cccgggacag aacagccccc 3541 tctgcaggca tgcagcccca gccttctctc tgctcctcag ccatgcgaaa acaggagatc 3601 attaagatta cagaacagct gattgaagcc atcaacaatg gggactttga ggcctacacg 3661 aagatttgtg atccaggcct cacttccttt gagcctgagg cccttggtaa cctcgtggag 3721 gggatggatt tccataagtt ttactttgag aatctcctgt ccaagaacag caagcctatc 3781 cataccacca tcctaaaccc acacgtccac gtgattgggg aggacgcagc gtgcatcgcc 3841 tacatccgcc tcacccagta catcgacggg cagggtcggc ctcgcaccag ccagtcagaa 3901 gagacccggg tctggcaccg tcgggatggc aagtggctca atgtccacta tcactgctca 3961 ggggcccctg ccgcaccgct gcagCTCGAG TACCCATACG ACGTACCTGA TTATGCAACT 4021 TATCCTTATG ATGTACCTGA TTATGCTAGC TATCCTTATG ATGTCCCAGA CTACGCATAG 4081 ggatccCTGC GATATCCAGC gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 4141 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 4201 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 4261 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 4321 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 4381 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 4441 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 4501 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 4561 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 4621 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 4681 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4741 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4801 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4861 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4921 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4981 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 5041 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 5101 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 5161 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 5221 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 5281 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 5341 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 5401 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 5461 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 5521 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 5581 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5641 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5701 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5761 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5821 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5881 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5941 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 6001 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 6061 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 6121 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 6181 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 6241 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 6301 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6361 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 6421 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 6481 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6541 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6601 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct 6661 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 6721 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt 6781 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 6841 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 6901 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 6961 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 7021 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 7081 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 7141 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 7201 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 7261 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 7321 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 7381 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 7441 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 7501 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 7561 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 7621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 7681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 7741 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 7801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 7861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 7921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 7981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 8041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8161 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 8221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8281 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8341 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8401 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 8461 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 8521 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 8581 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 8641 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8701 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 8761 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 8821 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 8881 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 8941 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9001 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9061 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9121 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9181 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9241 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9301 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9361 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9421 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 9481 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 9541 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 9601 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 9661 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 9721 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 9781 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 9841 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 9901 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 9961 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.