pCMV-3×FLAG-NPHS1-Neo plasmid

pCMV-3×FLAG-NPHS1-Neo plasmid

PVT106444

pCMV-3×FLAG-NPHS1-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-NPHS1(human)-Neo
Alias: NM_004646.4;CNF; nephrin; NPHN;
Gene lenght: 3792
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-NPHS1-Neo plasmid Description

pCMV-3×FLAG-NPHS1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-NPHS1-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg gccctgggga cgacgctcag ggcttctctc ctgctcctgg ggctgctgac 961 tgaaggcctg gcgGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA 1021 CAAGGATGAC GATGACAAGc agttggcgat tcctgcctcc gttccccggg gcttctgggc 1081 cctgcctgaa aacctgacgg tggtggaggg ggcctcagtg gagctgcgtt gtggggtcag 1141 cacccctggc agtgcggtgc aatgggccaa agatgggctg ctcctgggcc ccgaccccag 1201 gatcccaggc ttcccgaggt accgcctgga aggggaccct gctagaggtg aattccacct 1261 gcacatcgag gcctgtgacc tcagcgatga cgcggagtat gagtgccagg tcggccgctc 1321 tgagatgggg cccgagctcg tgtctcccag agtgatcctc tccatcctgg ttcctcccaa 1381 gctgctcctg ctgaccccag aggcaggcac catggtcacc tgggtagctg ggcaggagta 1441 cgtggtcaac tgtgtgtctg gggacgcgaa gccagcacct gacatcacca ttctcctgag 1501 tggacagaca atatctgaca tctctgcaaa cgtgaacgag ggctcccagc agaaactctt 1561 cactgtggag gccacagcca gggtgacacc ccggagctca gataataggc agttgctggt 1621 ctgtgaggcg tctagcccag cactggaggc ccccatcaag gcctcattca ccgtgaatgt 1681 tctgttccct ccaggacccc ctgtcatcga gtggccaggc ctggatgagg ggcacgtgcg 1741 ggcaggacag agcttggagc tgccgtgcgt ggcccgaggg ggtaatccct tagccacact 1801 gcagtggctg aagaatggcc agccggtgtc cacagcgtgg ggcacagagc acacccaggc 1861 ggtggcccgc agtgtgctgg tgatgaccgt gaggccagaa gaccatggag cgcagctcag 1921 ctgcgaggcc cacaacagcg tgtctgcagg gacccaggag cacggcatca cactgcaggt 1981 cacctttccc cctagtgcca ttattatctt gggatctgca tcccagactg agaacaagaa 2041 cgtgacactc tcctgtgtca gcaagtccag tcgcccgcgg gttctgctac gatggtggct 2101 gggctggcgg cagctgctgc ccatggagga gacagtcatg gatggactgc atggcggtca 2161 catctccatg tccaacctga cattcctggc gcggcgggag gacaacggtc tgaccctcac 2221 atgtgaggcc ttcagtgaag ccttcaccaa ggagaccttc aagaagtcgc tcatcctgaa 2281 cgtaaaatat cccgcccaga aactgtggat tgagggtccc ccagagggcc agaagctccg 2341 ggctgggacc cgggtgaggc tggtgtgttt ggctatcggg ggcaacccag agccctccct 2401 catgtggtac aaggactcgc gcaccgtgac cgagtcgcgg ctgccgcagg agtcgcggcg 2461 cgtgcatctc ggcagcgtgg agaaatctgg gagcaccttc tcccgagagc tggtgctggt 2521 cacagggccg tcggacaacc aggccaagtt cacgtgcaag gctggacagc tcagcgcgtc 2581 cacgcagctg gcggtgcagt ttcccccaac taacgtgacg atcctggcca acgcatccgc 2641 actgcgcccg ggagacgcct taaacttgac atgcgtcagc gtcagcagca atccgccggt 2701 caacttgtcc tgggacaagg aaggggagag gctggagggc gtggccgccc caccccggag 2761 agccccattc aaaggctccg ccgccgccag gagcgtcctt ctgcaagtgt catcccgcga 2821 tcatggccag cgcgtgacct gccgcgccca cagcgccgag ctccgcgaaa ccgtgagctc 2881 cttctatcgc ctcaacgtac tgtaccgtcc agagttcctg ggggagcagg tgctggtggt 2941 gaccgcggtg gagcagggcg aggcgttgct gcccgtgtcc gtgtccgcta accccgcccc 3001 cgaggccttc aactggacct tccgcggcta tcgcctcagt ccagcgggcg gcccccggca 3061 tcgcatcctg tccagcgggg ctctgcatct gtggaatgtg acccgcgcgg acgacggcct 3121 ctatcagctg cactgccaga actctgaggg caccgcggaa gcgcggctgc ggctggacgt 3181 gcactatgct cccaccatcc gtgccctcca ggaccccact gaggtgaacg tcgggggttc 3241 tgtggacata gtctgcactg tcgatgccaa tcccatcctc ccgggcatgt tcaactggga 3301 gagactggga gaagatgagg aggaccagag cctggatgac atggagaaga tatccagggg 3361 accaacgggg cgcctgcgga ttcaccatgc caaactggcc caggctggcg cttaccagtg 3421 cattgtggac aatggggtgg cgcctccagc acgacggctg ctccgtcttg ttgtcagatt 3481 tgccccccag gtggagcacc ccactcccct aactaaggtg gctgcagctg gagacagcac 3541 cagttctgcc accctccact gccgtgcccg aggtgtcccc aacatcgttt tcacttggac 3601 aaaaaacggg gtccctctgg atctccaaga tcccaggtac acggagcaca cataccacca 3661 gggtggtgtc cacagcagcc tcctgaccat tgccaacgtg tctgccgccc aggattacgc 3721 cctcttcaca tgtacagcca ccaacgccct tggctcggac caaaccaaca ttcaacttgt 3781 cagcatcagc cgccctgacc ctccatcagg attaaaggtt gtgagtctga ccccacactc 3841 cgtggggctg gagtggaagc ctggctttga tgggggcctg ccacagaggt tctgcatcag 3901 gtatgaggcc ctggggactc cagggttcca ctatgtggat gtcgtaccac cccaggccac 3961 caccttcacg ctgactggtc tacagccttc tacaagatac agggtctggc tgctggccag 4021 taatgccttg ggggacagtg gactggctga caaagggacc cagcttccca tcactacccc 4081 aggtctccac cagccttctg gagaacctga agaccagctg cccacagagc caccttcagg 4141 accctcgggg ctgcccctgc tgcctgtgct gttcgctctt ggggggcttc tgctcctctc 4201 caatgcctcc tgtgtcgggg gggtcctctg gcagcggaga ctcaggcgtc ttgctgaggg 4261 catctcagag aagacagagg cagggtcgga agaggaccga gtcaggaacg aatatgagga 4321 gagccagtgg acaggagagc gggacactca gagctccacg gtcagcacaa cagaggcaga 4381 gccgtattac cgctccctga gggacttcag cccccagctg cccccgacgc aggaggaggt 4441 gtcttattcc cgaggtttca caggtgaaga tgaggatatg gccttccctg ggcacttgta 4501 tgatgaggta gaaagaacgt accccccgtc tggagcctgg ggacccctct acgatgaagt 4561 gcagatggga ccctgggacc tccactggcc tgaagacaca tatcaggatc caagaggaat 4621 ctatgaccag gtggccggag acttggacac tctggaaccc gattctctgc ccttcgagct 4681 gaggggacat ctggtgtaaC TCGAGTCTAG AGGGCCCGTT TAAACCCGCT GATCAGCCTC 4741 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 4801 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 4861 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 4921 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA 4981 AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC 5041 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 5101 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 5161 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 5221 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 5281 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 5341 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 5401 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT 5461 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 5521 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 5581 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 5641 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 5701 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 5761 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 5821 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 5881 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 5941 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 6001 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 6061 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 6121 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 6181 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 6241 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 6301 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 6361 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 6421 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 6481 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 6541 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 6601 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 6661 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 6721 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 6781 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 6841 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 6901 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 6961 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 7021 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 7081 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 7141 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 7201 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 7261 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 7321 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 7381 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 7441 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 7501 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 7561 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 7621 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 7681 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 7741 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 7801 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 7861 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 7921 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 7981 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 8041 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 8101 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 8161 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 8221 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 8281 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 8341 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 8401 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 8461 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 8521 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 8581 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 8641 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 8701 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 8761 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 8821 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 8881 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 8941 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 9001 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 9061 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 9121 CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.