pLV3-CMV-3×FLAG-GSDMC-mTagBFP2

pLV3-CMV-3×FLAG-GSDMC-mTagBFP2-Puro plasmid

PVT106119

pLV3-CMV-3×FLAG-GSDMC-mTagBFP2-Puro plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-GSDMC(human)-mTagBFP2-Puro
Alias: NM_031415.3;MLZE
Gene lenght: 1524
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Blue
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×FLAG-GSDMC-mTagBFP2-Puro plasmid Description

pLV3-CMV-3×FLAG-GSDMC-mTagBFP2-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-3×FLAG-GSDMC-mTagBFP2-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggacta caaagaccat gacggtgatt ataaagatca 2341 tgacatcgac tacaaggatg acgatgacaa gctcgagatg ccctccatgt tggaacgcat 2401 tagcaaaaat ttggtcaaag agattggaag caaagacctg acacctgtca aatacctatt 2461 gagtgccacc aaattacgtc agtttgttat attacgaaag aagaaggatt ctcgttcatc 2521 attttgggaa caatctgact atgttccagt tgaattctcc ctcaatgaca tcctggagcc 2581 aagttcttca gtcctagaaa ctgttgtgac aggaccgttc cacttcagtg acattatgat 2641 ccagaagcat aaggctgaca tgggtgtgaa tgttggtata gaagtgagtg tgtcagggga 2701 ggcctctgtg gaccatggat gctccctcga gtttcaaatt gttaccatcc catcaccaaa 2761 cctggaagac tttcaaaaaa ggaaactgtt ggatccagag ccatcatttc tgaaggagtg 2821 ccggaggaga ggggacaacc tgtacgtggt gacagaggct gttgaactga tcaacaatac 2881 tgtgctgtac gatagcagta gtgtgaatat tttagggaaa attgctcttt ggattaccta 2941 tggcaagggt caaggccaag gagagagtct cagagtgaag aagaaggcgc tgactcttca 3001 gaaaggcatg gtgatggctt ataagagaaa gcagctggtt atcaaggaga aagccattct 3061 catctcagat gatgatgaac agagaacctt tcaagatgag tacgaaattt ccgaaatggt 3121 aggctactgt gctgcgagga gtgaggggtt gctaccatca tttcatacca tctctccaac 3181 cctcttcaat gcctcatcca atgatatgaa gttaaaacca gagctatttc tgacacagca 3241 atttttgagc gggcatttgc caaaatacga acaagttcac atcctcccag taggaagaat 3301 agaggaaccc ttctggcaaa atttcaagca tctacaagag gaggttttcc agaaaataaa 3361 gacactggct cagctctcaa aggatgttca ggatgtcatg ttctacagta tcctggccat 3421 gctcagagac agaggggctc tacaggacct gatgaacatg ctggaattgg acagctcagg 3481 tcatttggat ggccctggtg gtgccatcct aaagaaactt caacaggatt caaaccatgc 3541 atggtttaac ccaaaggacc ccattcttta tctccttgaa gccataatgg tgctgagtga 3601 cttccaacac gatttgctgg cctgttccat ggagaagagg atcctgcttc agcaacagga 3661 gctggtaagg agcatcctgg agccaaactt cagatacccc tggagcattc ccttcaccct 3721 caaacctgag ctcctcgccc cactccagag tgagggtttg gccatcacct atggcctgct 3781 ggaggagtgt ggccttagga tggagctgga taaccccagg tcaacctggg atgtagaagc 3841 aaagatgccc ctgtctgccc tctatgggac tctctcgttg ctgcagcagc tggctgaggc 3901 ctaggatccg cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg 3961 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta 4021 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc 4081 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa 4141 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc 4201 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc 4261 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc 4321 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg 4381 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag 4441 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gtgtctaagg gcgaagagct 4501 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt 4561 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa 4621 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta 4681 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt 4741 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc 4801 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt 4861 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac 4921 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct 4981 cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca agaaacccgc 5041 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga 5101 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct 5161 ccctagcaaa ctggggcaca agcttaatgg atctcgcgag ggcagaggaa gtcttctaac 5221 atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct 5281 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 5341 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 5401 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 5461 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 5521 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 5581 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 5641 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 5701 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 5761 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 5821 ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga 5881 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 5941 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 6001 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 6061 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 6121 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 6181 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 6241 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 6301 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 6361 gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc 6421 cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 6481 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa 6541 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6721 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6781 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 6961 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 7021 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 7081 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg 7141 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 7201 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 7261 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 7321 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 7381 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 7441 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 7501 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7561 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7621 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7681 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 7741 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7801 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 7861 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 7921 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 7981 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 8041 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 8101 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 8161 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 8221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 8281 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 8341 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 8401 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 8461 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 8521 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 8581 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 8641 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 8701 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 8761 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 8821 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 8881 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 8941 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 9001 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 9061 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 9121 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 9181 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 9241 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 9301 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt 9361 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 9421 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 9481 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 9541 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca 9601 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 9661 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag 9721 tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.