pLV3-CMV-SIM2-3×FLAG-CopGFP-Pu

pLV3-CMV-SIM2-3×FLAG-CopGFP-Puro plasmid

PVT105840

pLV3-CMV-SIM2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SIM2(human)-3×FLAG-CopGFP-Puro
Alias: NM_005069.6;bHLHe15; HMC13F06; HMC29C01; SIM
Gene lenght: 2001
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SIM2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SIM2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-SIM2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga aggagaagtc caagaatgcg 2341 gccaagacca ggagggagaa ggaaaatggc gagttttacg agcttgccaa gctgctcccg 2401 ctgccgtcgg ccatcacttc gcagctggac aaagcgtcca tcatccgcct caccacgagc 2461 tacctgaaga tgcgcgccgt cttccccgaa ggtttaggag acgcgtgggg acagccgagc 2521 cgcgccgggc ccctggacgg cgtcgccaag gagctgggat cgcacttgct gcagactttg 2581 gatggatttg tttttgtggt agcatctgat ggcaaaatca tgtatatatc cgagaccgct 2641 tctgtccatt taggcttatc ccaggtggag ctcacgggca acagtattta tgaatacatc 2701 catccttctg accacgatga gatgaccgct gtcctcacgg cccaccagcc gctgcaccac 2761 cacctgctcc aagagtatga gatagagagg tcgttctttc ttcgaatgaa atgtgtcttg 2821 gcgaaaagga acgcgggcct gacctgcagc ggatacaagg tcatccactg cagtggctac 2881 ttgaagatca ggcagtatat gctggacatg tccctgtacg actcctgcta ccagattgtg 2941 gggctggtgg ccgtgggcca gtcgctgcca cccagtgcca tcaccgagat caagctgtac 3001 agtaacatgt tcatgttcag ggccagcctt gacctgaagc tgatattcct ggattccagg 3061 gtgaccgagg tgacggggta cgagccgcag gacctgatcg agaagaccct ataccatcac 3121 gtgcacggct gcgacgtgtt ccacctccgc tacgcacacc acctcctgtt ggtgaagggc 3181 caggtcacca ccaagtacta ccggctgctg tccaagcggg gcggctgggt gtgggtgcag 3241 agctacgcca ccgtggtgca caacagccgc tcgtcccggc cccactgcat cgtgagtgtc 3301 aattatgtac tcacggagat tgaatacaag gaacttcagc tgtccctgga gcaggtgtcc 3361 actgccaagt cccaggactc ctggaggacc gccttgtcta cctcacaaga aactaggaaa 3421 ttagtgaaac ccaaaaatac caagatgaag acaaagctga gaacaaaccc ttacccccca 3481 cagcaataca gctcgttcca aatggacaaa ctggaatgcg gccagctcgg aaactggaga 3541 gccagtcccc ctgcaagcgc tgctgctcct ccagaactgc agccccactc agaaagcagt 3601 gaccttctgt acacgccatc ctacagcctg cccttctcct accattacgg acacttccct 3661 ctggactctc acgtcttcag cagcaaaaag ccaatgttgc cggccaagtt cgggcagccc 3721 caaggatccc cttgtgaggt ggcacgcttt ttcctgagca cactgccagc cagcggtgaa 3781 tgccagtggc attatgccaa ccccctagtg cctagcagct cgtctccagc taaaaatcct 3841 ccagagccac cggcgaacac tgctaggcac agcctggtgc caagctacga agcgcccgcc 3901 gccgccgtgc gcaggttcgg cgaggacacc gcgcccccga gcttcccgag ctgcggccac 3961 taccgcgagg agcccgcgct gggcccggcc aaagccgccc gccaggccgc ccgggacggg 4021 gcgcggctgg cgctggcccg cgcggcaccc gagtgctgcg cgcccccgac ccccgaggcc 4081 ccgggcgcgc cggcgcagct gcccttcgtg ctgctcaact accaccgcgt gctggcccgg 4141 cgcggaccgc tggggggcgc cgcacccgcc gcctccggcc tggcctgcgc tcccggcggc 4201 cccgaggcgg cgaccggcgc gctgcggctc cggcacccga gccccgccgc cacctccccg 4261 cccggcgcgc ccctgccgca ctacctgggc gcctcggtca tcatcaccaa cgggagggga 4321 tccGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC 4381 GATGACAAGt agcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 4441 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 4501 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 4561 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 4621 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 4681 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 4741 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 4801 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 4861 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 4921 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc 4981 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag 5041 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc 5101 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc 5161 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac 5221 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc 5281 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc 5341 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag 5401 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg 5461 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc 5521 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg 5581 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc 5641 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc 5701 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 5761 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 5821 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 5881 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 5941 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 6001 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 6061 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 6121 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 6181 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 6241 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 6301 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 6361 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 6421 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 6481 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 6541 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 6601 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 6661 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 6721 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaATCATC GTCCTTTCCT 6781 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 6841 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 6901 ccgcgtctTc gccttcgccc tcagacgagt cggatctccc tttggGccgc ctccccgcct 6961 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 7021 aaagggggga ctggaagggc taattcactc ccaacgaaGa taagatctgc tttttgcttg 7081 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 7141 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 7201 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 7261 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 7321 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 7381 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 7441 actcatcaat gtatcttatc atgtctggct ctagctaTCC CGCCCCTAAC TCCGCCCAtc 7501 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 7561 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 7621 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc 7681 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 7741 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 7801 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 7861 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 7921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 7981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 8041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 8101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 8161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 8221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 8281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 8341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 8401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 8461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 8521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 8581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 8641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 8701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 8761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 8821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 8881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 8941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 9001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 9061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 9121 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 9181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 9241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 9301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 9361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 9421 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 9481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 9541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 9601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 9661 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 9721 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 9781 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 9841 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 9901 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 9961 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 10021 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 10081 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 10141 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 10201 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 10261 acgttgtaaa acgacggcca gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.