pLV3-CMV-EPAS1-3×Myc-EF1a-CopG

pLV3-CMV-EPAS1-3×Myc-EF1a-CopGFP-Puro plasmid

PVT105816

pLV3-CMV-EPAS1-3×Myc-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-EPAS1(human)-3×Myc-EF1a-CopGFP-Puro
Alias: NM_001430.5;bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2
Gene lenght: 2610
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EPAS1-3×Myc-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-EPAS1-3×Myc-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-CMV-EPAS1-3×Myc-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatga cagctgacaa ggagaagaaa aggagtagct cggagaggag 2341 gaaggagaag tcccgggatg ctgcgcggtg ccggcggagc aaggagacgg aggtgttcta 2401 tgagctggcc catgagctgc ctctgcccca cagtgtgagc tcccatctgg acaaggcctc 2461 catcatgcga ctggcaatca gcttcctgcg aacacacaag ctcctctcct cagtttgctc 2521 tgaaaacgag tccgaagccg aagctgacca gcagatggac aacttgtacc tgaaagcctt 2581 ggagggtttc attgccgtgg tgacccaaga tggcgacatg atctttctgt cagaaaacat 2641 cagcaagttc atgggactta cacaggtgga gctaacagga catagtatct ttgacttcac 2701 tcatccctgc gaccatgagg agattcgtga gaacctgagt ctcaaaaatg gctctggttt 2761 tgggaaaaaa agcaaagaca tgtccacaga gcgggacttc ttcatgagga tgaagtgcac 2821 ggtcaccaac agaggccgta ctgtcaacct caagtcagcc acctggaagg tcttgcactg 2881 cacgggccag gtgaaagtct acaacaactg ccctcctcac aatagtctgt gtggctacaa 2941 ggagcccctg ctgtcctgcc tcatcatcat gtgtgaacca atccagcacc catcccacat 3001 ggacatcccc ctggatagca agaccttcct gagccgccac agcatggaca tgaagttcac 3061 ctactgtgat gacagaatca cagaactgat tggttaccac cctgaggagc tgcttggccg 3121 ctcagcctat gaattctacc atgcgctaga ctccgagaac atgaccaaga gtcaccagaa 3181 cttgtgcacc aagggtcagg tagtaagtgg ccagtaccgg atgctcgcaa agcatggggg 3241 ctacgtgtgg ctggagaccc aggggacggt catctacaac cctcgcaacc tgcagcccca 3301 gtgcatcatg tgtgtcaact acgtcctgag tgagattgag aagaatgacg tggtgttctc 3361 catggaccag actgaatccc tgttcaagcc ccacctgatg gccatgaaca gcatctttga 3421 tagcagtggc aagggggctg tgtctgagaa gagtaacttc ctattcacca agctaaagga 3481 ggagcccgag gagctggccc agctggctcc caccccagga gacgccatca tctctctgga 3541 tttcgggaat cagaacttcg aggagtcctc agcctatggc aaggccatcc tgcccccgag 3601 ccagccatgg gccacggagt tgaggagcca cagcacccag agcgaggctg ggagcctgcc 3661 tgccttcacc gtgccccagg cagctgcccc gggcagcacc acccccagtg ccaccagcag 3721 cagcagcagc tgctccacgc ccaatagccc tgaagactat tacacatctt tggataacga 3781 cctgaagatt gaagtgattg agaagctctt cgccatggac acagaggcca aggaccaatg 3841 cagtacccag acggatttca atgagctgga cttggagaca ctggcaccct atatccccat 3901 ggacggggaa gacttccagc taagccccat ctgccccgag gagcggctct tggcggagaa 3961 cccacagtcc accccccagc actgcttcag tgccatgaca aacatcttcc agccactggc 4021 ccctgtagcc ccgcacagtc ccttcctcct ggacaagttt cagcagcagc tggagagcaa 4081 gaagacagag cccgagcacc ggcccatgtc ctccatcttc tttgatgccg gaagcaaagc 4141 atccctgcca ccgtgctgtg gccaggccag cacccctctc tcttccatgg ggggcagatc 4201 caatacccag tggcccccag atccaccatt acattttggg cccacaaagt gggccgtcgg 4261 ggatcagcgc acagagttct tgggagcagc gccgttgggg ccccctgtct ctccacccca 4321 tgtctccacc ttcaagacaa ggtctgcaaa gggttttggg gctcgaggcc cagacgtgct 4381 gagtccggcc atggtagccc tctccaacaa gctgaagctg aagcgacagc tggagtatga 4441 agagcaagcc ttccaggacc tgagcggggg ggacccacct ggtggcagca cctcacattt 4501 gatgtggaaa cggatgaaga acctcagggg tgggagctgc cctttgatgc cggacaagcc 4561 actgagcgca aatgtaccca atgataagtt cacccaaaac cccatgaggg gcctgggcca 4621 tcccctgaga catctgccgc tgccacagcc tccatctgcc atcagtcccg gggagaacag 4681 caagagcagg ttccccccac agtgctacgc cacccagtac caggactaca gcctgtcgtc 4741 agcccacaag gtgtcaggca tggcaagccg gctgctcggg ccctcatttg agtcctacct 4801 gctgcccgaa ctgaccagat atgactgtga ggtgaacgtg cccgtgctgg gaagctccac 4861 gctcctgcaa ggaggggacc tcctcagagc cctggaccag gccaccggat ccGAACAAAA 4921 ACTAATATCA GAGGAAGATT TGGAACAGAA ACTAATCAGT GAAGAAGATT TAGAGCAAAA 4981 GTTAATTTCC GAGGAGGACT TATAgcggcc gcGaaggatc tgcgatcgct ccggtgcccg 5041 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa 5101 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg 5161 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa 5221 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat 5281 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt 5341 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa 5401 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg 5461 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt 5521 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag 5581 cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg 5641 cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa 5701 caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat 5761 gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca 5821 cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt 5881 gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt 5941 ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa 6001 cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg 6061 caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt 6121 caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg 6181 cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa 6241 gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac 6301 cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga 6361 cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg 6421 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac 6481 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt 6541 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt 6601 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc 6661 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct 6721 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga 6781 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg 6841 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg 6901 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat 6961 gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac 7021 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 7081 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 7141 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 7201 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 7261 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 7321 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 7381 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 7441 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 7501 tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc 7561 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 7621 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct 7681 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 7741 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 7801 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 7861 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 7921 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 7981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 8041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 8101 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 8161 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 8221 ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg 8281 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 8341 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 8401 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 8461 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 8521 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 8581 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 8641 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 8701 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 8761 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 8821 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 8881 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 8941 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 9001 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 9061 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 9121 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 9181 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 9241 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 9301 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 9361 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 9421 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 9481 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 9541 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 9601 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 9661 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 9721 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 9781 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 9841 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 9901 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 9961 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 10021 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 10081 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 10141 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 10201 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 10261 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 10321 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 10381 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 10441 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 10501 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 10561 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 10621 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 10681 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg 10741 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 10801 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 10861 tgtaaaacga cggccagtgc caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.