pLV3-ISRE-minP-Fluc-ISRE-mCher

pLV3-ISRE-minP-Fluc-ISRE-mCherry-Neo plasmid

PVT105632

pLV3-ISRE-minP-Fluc-ISRE-mCherry-Neo plasmid Information

Plasmid name: pLV3-ISRE-minP-Fluc-ISRE-mCherry-Neo
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red,Fluc
Promoter: minP
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: luc-F2
Reverse primer:
Copy number: High
Induction:

pLV3-ISRE-minP-Fluc-ISRE-mCherry-Neo plasmid Description

pLV3-ISRE-minP-Fluc-ISRE-mCherry-Neo It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV3-ISRE-minP-Fluc-ISRE-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtaaggatct gcgatcgctc cggtgcccgt cagtGAAACT GAAACTGAAA 1981 CTGAAACTGA AACTGAAACT GAAACTGAAA CTGAAACTGA AACTtcgagg aTAGAGGGTA 2041 TATAATGGAA GCTCGAATTC CAGccaagca ttcaagctgt gaccggcgcc tactctagag 2101 ctagcgaatt catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac 2161 tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc 2221 ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact 2281 tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc 2341 ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt 2401 tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca 2461 gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc 2521 tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg 2581 actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca 2641 acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga 2701 acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg 2761 tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta 2821 tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga 2881 tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct 2941 tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta 3001 agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg 3061 cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc 3121 gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg 3181 acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg 3241 acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga 3301 tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct 3361 ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc 3421 ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca 3481 tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg 3541 ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg 3601 agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg 3661 tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg 3721 agattctcat taaggccaag aagggcggca agatcgccgt gTGAttagcg gccgcGaagg 3781 atctgcgatc gctccggtgc ccgtcagtGA AACTGAAACT GAAACTGAAA CTGAAACTGA 3841 AACTGAAACT GAAACTGAAA CTGAAACTct gaagcttcga ggggctcgca tctctccttc 3901 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc 3961 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc 4021 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac 4081 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg 4141 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga gcaagggcga 4201 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 4261 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 4321 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 4381 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 4441 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 4501 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 4561 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 4621 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 4681 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 4741 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 4801 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 4861 ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag gaagtcttct 4921 aacatgcggt gacgtggagg agaatcccgg ccctATTGAA CAAGATGGAT TGCACGCAGG 4981 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 5041 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 5101 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 5161 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 5221 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 5281 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 5341 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 5401 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 5461 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 5521 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 5581 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 5641 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA 5701 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAgtcg acaatcaacc 5761 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5821 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5881 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 5941 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 6001 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 6061 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 6121 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 6181 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 6241 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 6301 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa 6361 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 6421 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt 6481 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 6541 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 6601 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 6661 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 6721 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6781 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6841 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc 6901 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 6961 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa 7021 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 7081 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 7141 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 7201 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7321 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7381 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7441 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7621 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7861 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 8041 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 8101 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8161 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8221 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8281 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8341 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8401 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8461 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8521 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8581 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8641 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8701 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 8761 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 8821 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 8881 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8941 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 9001 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 9061 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9181 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9241 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9301 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9361 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9421 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9481 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9541 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9601 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.