pCMV-Tmem94-3×FLAG-Neo plasmid

pCMV-Tmem94-3×FLAG-Neo plasmid

PVT105108

pCMV-Tmem94-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Tmem94(mouse)-3×FLAG-Neo
Alias: NM_028014.3;2310067B10Rik; ERMA; Kiaa0195; mKIAA0195
Gene lenght: 4080
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Tmem94-3×FLAG-Neo plasmid Description

pCMV-Tmem94-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Tmem94-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatgga cctgagggag aagcacctgg 781 gagagccccc cttggccctg ggcctgtcta cccggaaggc cctcagtgtc ctgaaggagc 841 agctggaggc tgtattggag aaacacctca aagaacggaa gaaaagtctt acatggaagg 901 aggcatggag aagcagcttc ctgcacctca gtaaccgctg ctcctgtttc cactggcccg 961 gggcctccct gatgctgctg gcggtgctgc tgctgttgtg ttgctgtggg ggccagccag 1021 ctgggagcca gggcgtggag ctggtgaacg cctctgctct gttcctgttg ctgctcctca 1081 acctcgtcct catcgggcgg caagaccggc tgaagcgccg ggaggtggag cgcaggctcc 1141 gggggatcat cgaccaaatc caagatgctc tcagggatgg aaaagagatc aagtggccga 1201 actccatgta tccagacctc catatgccct ttgcaccttc ctggtccctg cactgggctt 1261 acagagatgg acacctggtg aacctgccag ttagcctgtt ggtagaagga gacatcatag 1321 ccctgaggcc tggccaggag tcctttgcct ccctgagggg catcaaggac gatgagcaca 1381 ttgtcttgga gccgggagac ctgtttcccc ccttctctcc accaccatcg ccccggggag 1441 aagtaaagag agggccacag aacccccagc aacaccggct cttccgagtc ctggagacgc 1501 ctgtgattga caacatcaga tggtgcctgg acacagccct gtcccgcccc gtcacggctc 1561 tggacaatga aaggttcaca gtacagtcgg tgatgcttca ctatgccgtg ccggtggtcc 1621 tggctggctt tctcatcacc aatgccctgc gcttcatgtt caaggcacct ggggtcacgt 1681 cctggcagta cacccttctc cagctccagg tgaacggcat gttgcccatc ctccccctgc 1741 tcttcccggt cctctgggtg ctggcgaccg cctgtggaga agctcgtgtc ctggcccaga 1801 tgagcaaggc ttcccccagc tccctgctgg ccaagttctc agaggacact ctcagcagct 1861 acactgaagc cgtctcctct caggagatgc tgcgctgcat ttggggtcac tttctgaggg 1921 tgatccaggg cacatcgcca actctgagcc acagtgccag tctgctgcac agcttgggct 1981 ccgttacggt actgtgctgt gtagacaaac aggggatcct atcatggcca aaccccagcc 2041 cagagaccgt gctcttcttc agtgggaagg tggagccccc tcacagcagc cacgaggacc 2101 tcacagacga cctgtccacc cgttccttct gccatcccga ggtggaggag gagccccatg 2161 aacacgatgc cctccttgct ggctccctga acaataccct gcacctttcc aacgagcagg 2221 agcgcagtga ctggcttgcc gacggcccca agccctctga gccctaccct catcataaag 2281 gacatggccg cagcaaacac ccgtctggct ccaacgtgag tttcagcagg gacactgaag 2341 gcggagagga agagcccagc aaggctcagc ctgggacaga gggtgatccc tacgaggccg 2401 aggacttcgt gtgtgactac cacttggaga tgctgagcct gtcccaggac caacagaacc 2461 cctcgtgcat acagttcgat gattccaact ggcagtcaca cctgacctcc ctcaagccgc 2521 tgggcctcaa cgtgctgctg aacctgtgta acgccagtgt cactgagcgc ctgtgccgct 2581 tttccgacca cctgtgcaac atcgccctgc aggagagcca cagcgctgtg ctgcccgtgc 2641 acgtgccctg gggcctctgc gagctggccc ggctcatagg tttcactcca ggggccaagg 2701 agctcttcaa gcaggagaac cacctggcgc tctaccgcct ccccagtgca gagaccctga 2761 aggagacgtc attggggagg ccctcctgtg tcaccaagcg gcgccccccg ctcagccaca 2821 tgatcagcct cttcatcaaa gacactgcca ccagcacaga gcagatgctg tcccatggca 2881 gtgccgacgt ggtcgtggag gcctgcacag acttctggga tggcgctgac atctaccctc 2941 tgtcgggttc agacagaaag aaagtgctgg acttctacca gcgagcctgc ctatccggct 3001 attgctctgc ctttgcctac aagcccatga actgcacgct gtcctctcag ctcaacggca 3061 agtgcatcga gctggtgcag gtccccggcc agaacagcat attcaccatg tgcgagctgc 3121 ccagcaccat ccccatcaag ccaaacaacc gccgcagcag ctggagctcc gatgaaggga 3181 tcggggaggt gctggagaaa gaagactgca tgcaggccct gagcggtcag atcttcatgg 3241 gcatggtgtc ctcccagtac caggcccggc tggacatcgt gcgcctcatc gatgggctgg 3301 tcaacgcctg catccgcttt gtgtacttct ctttggaaga tgagctcagg agcaaggtgt 3361 ttgcagagaa gatgggcctg gagacaggct ggaactgcca catctccctc acgcccaacg 3421 gtgacatgcc tggctctgag atccctccct ccagccccag ccatgctggc tctctccatg 3481 acgacctgaa tcaggtgtcc cgagatgatg cagaagggct cctcctctta gaggaggagg 3541 gtcactcaga cctcatcagc ttccagccca ccgacagcga tatccccagc ttcctggaag 3601 actgcaaccg ggccaagctg ccccggggca tccaccaggt gcggccccac ctacagaaca 3661 tcgacaatgt accgttgctg gtgcccctct tcaccgactg cacccctgac accatgtgtg 3721 agatgataaa gatcatgcag gagtatgggg aggtgacttg ctgcctgggg agctcggcca 3781 acctgcgcaa cagctgcctc ttcctccaga gtgatgtcag cattgccctg gatcccttat 3841 acccatcccg ctgctcctgg gagacctttg gctatgccac cagcaccacc atggcccagg 3901 cctcggatgg cctttctccc ctgcagctat cagggcagct caacagcctg ccttgctccc 3961 tgaccttccg acaagaagaa agcatcagca tcatccggct cattgagcag gctcggcacg 4021 ctacctacgg catccgaaag tgcttcctct tcctgttgca gtgccagctg accctcgtgg 4081 tcattcagtt cctgtcttgt ctggtgcagc tgccaccgct cctgagtacc acagacattt 4141 tatggctatc ctgcttttgt taccctctgc tcagcatctc tctattggga aaaccaccac 4201 atagctccat catgtctatg gcaacgggca aaaaccttca gtccattcct aagaagaccc 4261 agcactactt cctgctctgc ttcctgctca agttcagcct caccatcagc tcctgcctcg 4321 tctgcttcgg cttcacgctg cagagctttt gtgacagcgc ccgggcccgc aacctcacca 4381 actgctcctc agtcatgctt tgcagcaatg atgacagagc gccggcctgg ttcgaggatt 4441 ttgccaatgg gttgctatcc gctcagaagc tcacagccgc cctgattgtt ttgcacactg 4501 tctttatctc catcactcat gtacaccgca ccaagcctct gtggagaaag agccctttga 4561 caaacctctg gtgggccgtg actgtgcctg tggtgctgct cggtcaggtg gtccagacag 4621 tggtggacct gcagctgtgg acacacaggg actcacgtgt ccactttggc ctagaagatg 4681 tgcccctgct cacatggctc ctgggctgcc tgtccctggt cctagtggta gtcaccaacg 4741 agattgtaaa gttacacgag attcgggtcc gtgtccgcta ccagaagcgc cagaagctgc 4801 agtttgagac caagctgggc atgaactcgc ccttcGGATC CGACTACAAA GACCATGACG 4861 GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG TGAGGGCCCG 4921 ATATCTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA 4981 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT 5041 TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA 5101 TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC 5161 AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT 5221 CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG 5281 GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC 5341 ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC 5401 TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT 5461 TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC 5521 TGGAAACGGG GGAGGCTAAC TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT 5581 ATGACGGCAA TAAAAAGACA GAATAAAACG CACGGGTGTT GGGTCGTTTG TTCATAAACG 5641 CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA 5701 ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG 5761 GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCA GATCTGCGCA GCTGGGGCTC 5821 TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 5881 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 5941 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT 6001 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 6061 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 6121 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 6181 TTCTTTTGAT TTATAAGGGA TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 6241 TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 6301 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 6361 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 6421 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 6481 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 6541 GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 6601 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 6661 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 6721 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 6781 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 6841 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 6901 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 6961 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 7021 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 7081 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 7141 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 7201 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 7261 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 7321 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 7381 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 7441 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGCGAAA TGACCGACCA 7501 AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 7561 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 7621 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 7681 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 7741 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT 7801 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 7861 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 7921 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 7981 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 8041 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 8101 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 8161 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 8221 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 8281 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 8341 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 8401 GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 8461 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 8521 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 8581 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 8641 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 8701 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 8761 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 8821 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 8881 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 8941 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 9001 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 9061 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 9121 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 9181 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 9241 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 9301 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 9361 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 9421 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 9481 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 9541 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 9601 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 9661 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 9721 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 9781 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 9841 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 9901 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 9961 ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC 10021 TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT 10081 GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG 10141 AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.