pLV3-CMV-HOXA1-3×Myc-mCherry-P

pLV3-CMV-HOXA1-3×Myc-mCherry-Puro plasmid

PVT104991

pLV3-CMV-HOXA1-3×Myc-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-HOXA1(human)-3×Myc-mCherry-Puro
Alias: NM_005522.5;BSAS; HOX1; HOX1F
Gene lenght: 1005
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HOXA1-3×Myc-mCherry-Puro plasmid Description

pLV3-CMV-HOXA1-3×Myc-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HOXA1-3×Myc-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggacaa tgcaagaatg aactccttcc tggaataccc 2341 catacttagc agtggcgact cggggacctg ctcagcccga gcctacccct cggaccatag 2401 gattacaact ttccagtcgt gcgcggtcag cgccaacagt tgcggcggcg acgaccgctt 2461 cctagtgggc aggggggtgc agatcggttc gccccaccac caccaccacc accaccatcg 2521 ccacccccag ccggctacct accagacttc cgggaacctg ggggtgtcct actcccactc 2581 aagttgtggt ccaagctatg gctcacagaa cttcagtgcg ccttacagcc cctacgcgtt 2641 aaatcaggaa gcagacgtaa gtggtgggta cccccagtgc gctcccgctg tttactctgg 2701 aaatctctca tctcccatgg tccagcatca ccaccaccac cagggttatg ctgggggcgc 2761 ggtgggctcg cctcaataca ttcaccactc atatggacag gagcaccaga gcctggccct 2821 ggctacgtat aataactcct tgtcccctct ccacgccagc caccaagaag cctgtcgctc 2881 ccccgcatcg gagacatctt ctccagcgca gacttttgac tggatgaaag tcaaaagaaa 2941 ccctcccaaa acagggaaag ttggagagta cggctacctg ggtcaaccca acgcggtgcg 3001 caccaacttc actaccaagc agctcacgga actggagaag gagttccact tcaacaagta 3061 cctgacgcgc gcccgcaggg tggagatcgc tgcatccctg cagctcaacg agacccaagt 3121 gaagatctgg ttccagaacc gccgaatgaa gcaaaagaaa cgtgagaagg agggtctctt 3181 gcccatctct ccggccaccc cgccaggaaa cgacgagaag gccgaggaat cctcagagaa 3241 gtccagctct tcgccctgcg ttccttcccc ggggtcttct acctcagaca ctctgactac 3301 ctcccacctc gagGAACAAA AACTAATATC AGAGGAAGAT TTGGAACAGA AACTAATCAG 3361 TGAAGAAGAT TTAGAGCAAA AGTTAATTTC CGAGGAGGAC TTAtaggatc cgcggccgcG 3421 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc 3481 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt 3541 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc 3601 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac 3661 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg 3721 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac 3781 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct 3841 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca 3901 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc 3961 gcctacgcta gacgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa 4021 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 4081 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 4141 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 4201 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 4261 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 4321 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 4381 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 4441 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 4501 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 4561 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 4621 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 4681 gtacaaggga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa 4741 tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 4801 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 4861 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 4921 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 4981 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 5041 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 5101 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 5161 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 5221 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 5281 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 5341 cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 5401 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 5461 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 5521 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 5581 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 5641 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 5701 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc 5761 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 5821 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 5881 tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct 5941 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 6001 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg 6061 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 6121 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 6181 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 6241 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 6301 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 6361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 6421 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt 6481 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 6541 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 6601 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 6661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 6721 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 6781 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 6841 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 6901 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 6961 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 7021 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 7081 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 7141 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 7201 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 7261 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 7321 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 7381 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 7441 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 7501 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 7561 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 7621 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 7681 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 7741 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 7801 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 7861 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 7921 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 7981 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 8041 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 8101 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 8161 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 8221 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 8281 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 8341 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 8401 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 8461 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 8521 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 8581 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 8641 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 8701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 8761 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 8821 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac 8881 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 8941 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat 9001 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 9061 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg 9121 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 9181 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 9241 gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.