pLV3-CMV-OASL-3×FLAG-CopGFP-Pu

pLV3-CMV-OASL-3×FLAG-CopGFP-Puro plasmid

PVT104831

pLV3-CMV-OASL-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-OASL(human)-3×FLAG-CopGFP-Puro
Alias: NM_003733.4;OASL1; OASLd; p59 OASL; p59-OASL; p59OASL;TRIP-14; TRIP14
Gene lenght: 1542
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-OASL-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-OASL-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-OASL-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cactgatgca ggaactgtat 2341 agcacaccag cctccaggct ggactccttc gtggctcagt ggctgcagcc ccaccgggag 2401 tggaaggaag aggtgctaga cgctgtgcgg accgtggagg agtttctgag gcaggagcat 2461 ttccagggga agcgtgggct ggaccaggat gtgcgggtgc tgaaggtagt caaggtgggc 2521 tccttcggga atggcacggt tctcaggagc accagagagg tggagctggt ggcgtttctg 2581 agctgtttcc acagcttcca ggaggcagcc aagcatcaca aagatgttct gaggctgata 2641 tggaaaacca tgtggcaaag ccaggacctg ctggacctcg ggctcgagga cctgaggatg 2701 gagcagagag tccccgatgc tctcgtcttc accatccaga ccagggggac tgcggagccc 2761 atcacggtca ccattgtgcc tgcctacaga gccctggggc cttctcttcc caactcccag 2821 ccaccccctg aggtctatgt gagcctgatc aaggcctgcg gtggtcctgg aaatttctgc 2881 ccatccttca gcgagctgca gagaaatttc gtgaaacatc ggccaactaa gctgaagagc 2941 ctcctgcgcc tggtgaaaca ctggtaccag cagtatgtga aagccaggtc ccccagagcc 3001 aatctgcccc ctctctatgc tcttgaactt ctaaccatct atgcctggga aatgggtact 3061 gaagaagacg agaatttcat gttggacgaa ggcttcacca ctgtgatgga cctgctcctg 3121 gagtatgaag tcatctgtat ctactggacc aagtactaca cactccacaa tgcaatcatt 3181 gaggattgtg tcagaaaaca gctcaaaaaa gagaggccca tcatcctgga tccggccgac 3241 cccaccctca acgtggcaga agggtacaga tgggacatcg ttgctcagag ggcctcccag 3301 tgcctgaaac aggactgttg ctatgacaac agggagaacc ccatctccag ctggaacgtg 3361 aagagggcac gagacatcca cttgacagtg gagcagaggg gttacccaga tttcaacctc 3421 atcgtgaacc cttatgagcc cataaggaag gttaaagaga aaatccggag gaccaggggc 3481 tactctggcc tgcagcgtct gtccttccag gttcctggca gtgagaggca gcttctcagc 3541 agcaggtgct ccttagccaa atatgggatc ttctcccaca ctcacatcta tctgctggag 3601 accatcccct ccgagatcca ggtcttcgtg aagaatcctg atggtgggag ctacgcctat 3661 gccatcaacc ccaacagctt catcctgggt ctgaagcagc agattgaaga ccagcagggg 3721 cttcctaaaa agcagcagca gctggaattc caaggccaag tcctgcagga ctggttgggt 3781 ctggggatct atggcatcca agacagtgac actctcatcc tctcgaagaa gaaaggagag 3841 gctctgtttc cagccagtgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3901 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 3961 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 4021 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 4081 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4141 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4201 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 4261 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4321 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4381 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4441 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 4501 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 4561 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 4621 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 4681 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 4741 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 4801 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4861 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4921 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4981 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 5041 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 5101 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 5161 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 5221 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 5281 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 5341 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5401 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5461 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5521 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5581 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5641 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5701 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5761 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5821 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5881 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 5941 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 6001 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 6061 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6121 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6181 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6241 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6301 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 6361 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6421 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 6481 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 6541 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 6601 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6781 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6841 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 7021 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 7081 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7141 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 7201 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7261 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 7321 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7381 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 7561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 8041 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8281 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8341 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8401 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8461 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 8521 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 8581 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8641 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8701 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8761 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8821 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8881 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8941 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 9001 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 9061 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9121 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9181 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9241 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9301 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9361 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9421 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 9481 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 9541 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 9601 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 9661 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 9721 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 9781 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.