pCMV-3×FLAG-GPR39-Neo plasmid

pCMV-3×FLAG-GPR39-Neo plasmid

PVT103835

pCMV-3×FLAG-GPR39-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-GPR39(human)-Neo
Alias: NM_001508.3;ZnR;
Gene lenght: 1362
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-GPR39-Neo plasmid Description

pCMV-3×FLAG-GPR39-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-GPR39-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atggcttcac ccagcctccc gggcagtgac 1021 tgctcccaaa tcattgatca cagtcatgtc cccgagtttg aggtggccac ctggatcaaa 1081 atcaccctta ttctggtgta cctgatcatc ttcgtgatgg gccttctggg gaacagcgcc 1141 accattcggg tcacccaggt gctgcagaag aaaggatact tgcagaagga ggtgacagac 1201 cacatggtga gtttggcttg ctcggacatc ttggtgttcc tcatcggcat gcccatggag 1261 ttctacagca tcatctggaa tcccctgacc acgtccagct acaccctgtc ctgcaagctg 1321 cacactttcc tcttcgaggc ctgcagctac gctacgctgc tgcacgtgct gacactcagc 1381 tttgagcgct acatcgccat ctgtcacccc ttcaggtaca aggctgtgtc gggaccttgc 1441 caggtgaagc tgctgattgg cttcgtctgg gtcacctccg ccctggtggc actgcccttg 1501 ctgtttgcca tgggtactga gtaccccctg gtgaacgtgc ccagccaccg gggtctcact 1561 tgcaaccgct ccagcacccg ccaccacgag cagcccgaga cctccaatat gtccatctgt 1621 accaacctct ccagccgctg gaccgtgttc cagtccagca tcttcggcgc cttcgtggtc 1681 tacctcgtgg tcctgctctc cgtagccttc atgtgctgga acatgatgca ggtgctcatg 1741 aaaagccaga agggctcgct ggccgggggc acgcggcctc cgcagctgag gaagtccgag 1801 agcgaagaga gcaggaccgc caggaggcag accatcatct tcctgaggct gattgttgtg 1861 acattggccg tatgctggat gcccaaccag attcggagga tcatggctgc ggccaaaccc 1921 aagcacgact ggacgaggtc ctacttccgg gcgtacatga tcctcctccc cttctcggag 1981 acgtttttct acctcagctc ggtcatcaac ccgctcctgt acacggtgtc ctcgcagcag 2041 tttcggcggg tgttcgtgca ggtgctgtgc tgccgcctgt cgctgcagca cgccaaccac 2101 gagaagcgcc tgcgcgtaca tgcgcactcc accaccgaca gcgcccgctt tgtgcagcgc 2161 ccgttgctct tcgcgtcccg gcgccagtcc tctgcaagga gaactgagaa gattttctta 2221 agcacttttc agagcgaggc cgagccccag tctaagtccc agtcattgag tctcgagtca 2281 ctagagccca actcaggcgc gaaaccagcc aattctgctg cagagaatgg ttttcaggag 2341 catgaagttt gagaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg 2401 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 2461 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 2521 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 2581 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 2641 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 2701 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 2761 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 2821 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 2881 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 2941 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 3001 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 3061 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 3121 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 3181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 3421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 3481 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 3541 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 3601 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 3661 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 3721 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 3781 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 3841 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 3901 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 3961 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 4021 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 4081 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 4141 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 4201 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 4261 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 4321 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 4381 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 4441 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 4501 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 4561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 4621 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 4681 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 4741 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 4801 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 4861 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 4921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 4981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 5041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 5101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 5161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 5221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 5281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 5341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 5401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 5461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 5521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 5581 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac 5641 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 5701 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 5761 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 5821 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 5881 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 5941 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 6001 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 6061 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 6121 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 6181 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 6241 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 6301 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 6361 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 6421 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 6481 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 6541 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 6601 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 6661 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 6721 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 6781 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.