pAAV-Trex1-3×FLAG-P2A-EGFP pla

pAAV-Trex1-3×FLAG-P2A-EGFP plasmid

PVT103493

pAAV-Trex1-3×FLAG-P2A-EGFP plasmid Information

Plasmid name: pAAV-Trex1(mouse)-3×FLAG-P2A-EGFP
Alias: NM_011637.6
Gene lenght: 942
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PAAV-MCS-F
Reverse primer:
Copy number: Low
Induction:

pAAV-Trex1-3×FLAG-P2A-EGFP plasmid Description

pAAV-Trex1-3×FLAG-P2A-EGFP This is a plasmid suitable for protein expression in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-Trex1-3×FLAG-P2A-EGFP plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt cgcGCCACCa tgggctcaca gaccctgccc catggtcaca tgcagaccct 1381 catcttctta gacctggaag ccactggcct gccttcgtct cggcccgaag tcacagagct 1441 gtgcctgctg gctgtccaca gacgtgctct ggagaacact tccatttctc agggacatcc 1501 acctccagtg cccagaccgc cccgtgtggt ggacaagctc tctctgtgca ttgctccagg 1561 gaaagcctgt agccctgggg ccagtgagat cacaggtctg agcaaagctg agctggaagt 1621 acaggggcgt caacgcttcg atgacaacct ggccatcctg ctccgagcct tcctgcagcg 1681 ccagccacag ccttgctgcc ttgtggcaca caacggtgac cgctatgact ttcctctgct 1741 ccagacagag cttgctaggc tgagcactcc cagtccccta gatggtacct tctgtgtgga 1801 cagcatcgct gccctaaagg ccttggaaca agctagcagc ccctcaggga atggttcgag 1861 gaaaagctac agcctgggca gcatctacac ccgcctgtac tggcaagcac cgacagactc 1921 acatactgct gaaggtgatg ttctaaccct gctcagcatc tgtcagtgga agccacaggc 1981 cctactgcag tgggtggacg aacatgcccg gccctttagc accgtcaagc ccatgtacgg 2041 cactccggct accactggaa caaccaacct aaggccacat gctgccacag ctactacacc 2101 cctggccaca gccaatggaa gtcccagcaa tggcaggagc aggcgaccta agagtcctcc 2161 tccagagaag gtcccagaag ccccatcaca ggaggggctg ctggccccac tgagcctgct 2221 gaccctcctg accttggcaa tagccactct gtatggactc ttcctggcct cacctgggca 2281 ggtcgacGCC ACCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA 2341 CTACAAGGAT GACGATGACA AGGGAAGCGG AGCCACTAAC TTCTCCCTGT TGAAACAAGC 2401 AGGGGATGTC GAAGAGAATC CCGGGCCACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA 2461 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 2521 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT 2581 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA 2641 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 2701 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA 2761 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 2821 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA 2881 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA 2941 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC 3001 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC 3061 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC 3121 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA TGAGGGCCCG ATATCTCGAC 3181 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 3241 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 3301 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 3361 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 3421 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 3481 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 3541 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC 3601 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 3661 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 3721 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCa agcttgcctc 3781 gagcagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct 3841 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg 3901 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg 3961 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag 4021 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat 4081 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta 4141 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc 4201 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac 4261 cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg 4321 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag 4381 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag 4441 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc 4501 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa 4561 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 4621 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 4681 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 4741 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 4801 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 4861 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct 4921 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 4981 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 5041 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat 5101 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 5161 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg 5221 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa 5281 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 5341 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 5401 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 5461 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 5521 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 5581 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 5641 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 5701 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 5761 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 5821 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 5881 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 5941 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 6001 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 6061 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 6121 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 6181 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 6241 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 6301 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 6361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 6421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 6481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 6541 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 6601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 6661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 6721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 6781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 6841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 6901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 6961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 7021 tacggttcct ggccttttgc tggccttttg ctcacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.