pCMV-TMEM41B-mut-EGFP-Hotag3-C

pCMV-TMEM41B-mut-EGFP-Hotag3-CD274-HOTag6-Neo plasmid

PVT103377

pCMV-TMEM41B-mut-EGFP-Hotag3-CD274-HOTag6-Neo plasmid Information

Plasmid name: pCMV-TMEM41B(human)-mut-EGFP-Hotag3-CD274(human)-HOTag6-Neo
Alias: NM_014143.4;B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1;PDCD1LG1; PDL1;
Gene lenght: 870
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-TMEM41B-mut-EGFP-Hotag3-CD274-HOTag6-Neo plasmid Description

pCMV-TMEM41B-mut-EGFP-Hotag3-CD274-HOTag6-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TMEM41B-mut-EGFP-Hotag3-CD274-HOTag6-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCATG GCGAAAGGCA GAGTCGCCGA ACGATCGCAG TTGGGCGCTC ACCACACGAC 961 CCCCGTGGGG GACGGGGCAG CGGGGACGCG GGGTCTCGCG GCGCCTGGCA GCAGAGACCA 1021 CCAGAAGGAA AAATCCTGGG TAGAAGCTGG ATCAGCAAGA ATGTCACTCC TTATATTGGT 1081 GTCCATTTTC TTATCTGCAG CTTTTGTTAT GTTTTTGGTA TATAAAAATT TTCCTCAGCT 1141 TAGTGAAGAA GAAAGAGTGA ATATGAAGGT TCCCAGAGAT ATGGATGATG CCAAGGCTCT 1201 AGGAAAAGTT TTATCCAAAT ACAAGGACAC CTTTTATGTT CAAGTACTTG TAGCTTATTT 1261 TGCTACATAT ATTTTCTTGC AAACATTTGC TATTCCAGGC TCTATATTTC TCAGTATACT 1321 CTCAGGGTTT CTTTATCCCT TTCCACTAGC CTTATTTCTT GTTTGTTTGT GTTCTGGACT 1381 TGGTGCCTCT TTCTGTTATA TGCTTTCCTA TTTAGTTGGG AGACCAGTTG TATACAAATA 1441 CCTAACAGAG AAAGCAGTAA AATGGTCACA GCAGGTTGAA CGTCATAGAG AACATCTCAT 1501 TAACTACATT ATATTTTTGA GAATAACACC ATTTCTGCCT AATTGGTTTA TTAATATCAC 1561 ATCTCCTGTG ATAAACGTGC CATTGAAAGT TTTTTTTATT GGTACTTTTC TAGGTGTCGC 1621 ACCTCCTTCT TTTGTAGCCA TTAAGGCAGG AACAACACTG TATCAACTTA CAACAGCAGG 1681 AGAAGCTGTT TCCTGGAACT CAATATTTAT TCTGATGATC TTGGCTGTTC TTTCTATTCT 1741 GCCAGCCATC TTCCAAAAAA AACTAAAGCA GAAATTTGAG ctcgagtccg gactcagatc 1801 tggcagcggt ggaggcgata tcatggtgag caagggcgag gagctgttca ccggggtggt 1861 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 1921 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 1981 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 2041 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 2101 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 2161 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 2221 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 2281 catggccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga 2341 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 2401 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 2461 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 2521 catggacgag ctgtacaagG GATCCGGCAG TGCTGGTGGT AGTGCTGGTG GTAGTGCTGG 2581 TGGTAGTGCT GGTGGCAGTG CTGGTGGTAG TGCTGGTGGT AGTGCTGGTG GCTCTAGAgg 2641 agaaatcgca aagagtctga aagaaattgc aaaatccttg aaggaaatag cgtggtcact 2701 gaaagaaatt gccaagtccc tcaaaggggg cagtggagag ggcagaggaa gtctgctaac 2761 atgcggtgac gtcgaggaga atcctggccc aATGAGGATA TTTGCTGTCT TTATATTCAT 2821 GACCTACTGG CATTTGCTGA ACGCATTTAC TGTCACGGTT CCCAAGGACC TATATGTGGT 2881 AGAGTATGGT AGCAATATGA CAATTGAATG CAAATTCCCA GTAGAAAAAC AATTAGACCT 2941 GGCTGCACTA ATTGTCTATT GGGAAATGGA GGATAAGAAC ATTATTCAAT TTGTGCATGG 3001 AGAGGAAGAC CTGAAGGTTC AGCATAGTAG CTACAGACAG AGGGCCCGGC TGTTGAAGGA 3061 CCAGCTCTCC CTGGGAAATG CTGCACTTCA GATCACAGAT GTGAAATTGC AGGATGCAGG 3121 GGTGTACCGC TGCATGATCA GCTATGGTGG TGCCGACTAC AAGCGAATTA CTGTGAAAGT 3181 CAATGCCCCA TACAACAAAA TCAACCAAAG AATTTTGGTT GTGGATCCAG TCACCTCTGA 3241 ACATGAACTG ACATGTCAGG CTGAGGGCTA CCCCAAGGCC GAAGTCATCT GGACAAGCAG 3301 TGACCATCAA GTCCTGAGTG GTAAGACCAC CACCACCAAT TCCAAGAGAG AGGAGAAGCT 3361 TTTCAATGTG ACCAGCACAC TGAGAATCAA CACAACAACT AATGAGATTT TCTACTGCAC 3421 TTTTAGGAGA TTAGATCCTG AGGAAAACCA TACAGCTGAA TTGGTCATCC CAGAACTACC 3481 TCTGGCACAT CCTCCAAATG AAAGGACTCA CTTGGTAATT CTGGGAGCCA TCTTATTATG 3541 CCTTGGTGTA GCACTGACAT TCATCTTCCG TTTAAGAAAA GGGAGAATGA TGGATGTGAA 3601 AAAATGTGGC ATCCAAGATA CAAACTCAAA GAAGCAAAGT GATACACATT TGGAGGAGAC 3661 Gggatccggc agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg ctggtggcag 3721 tgctggtggt agtgctggtg gtagtgctgg tggctctaga ACCTTGAGAG AAATCGAGGA 3781 ATTGTTGAGG AAAATCATTG AAGACAGCGT GCGCAGTGTG GCCGAGCTGG AGGACATTGA 3841 GAAGTGGCTG AAGAAAATCt agCTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC 3901 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 3961 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 4021 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 4081 GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC 4141 GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG 4201 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 4261 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 4321 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 4381 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG 4441 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC 4501 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 4561 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG 4621 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 4681 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 4741 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 4801 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 4861 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 4921 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 4981 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 5041 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 5101 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 5161 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 5221 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 5281 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 5341 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 5401 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 5461 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 5521 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 5581 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 5641 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 5701 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 5761 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 5821 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 5881 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 5941 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 6001 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 6061 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 6121 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 6181 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 6241 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 6301 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 6361 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 6421 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 6481 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 6541 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 6601 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 6661 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 6721 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 6781 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 6841 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 6901 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 6961 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 7021 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 7081 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 7141 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 7201 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 7261 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 7321 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 7381 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 7441 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 7501 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 7561 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 7621 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 7681 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 7741 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 7801 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 7861 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 7921 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 7981 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 8041 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 8101 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 8161 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 8221 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 8281 TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.