pAAV-EF1a-M-HBsAg plasmid

pAAV-EF1a-M-HBsAg plasmid

PVT102915

pAAV-EF1a-M-HBsAg plasmid Information

Plasmid name: pAAV-EF1a-M-HBsAg
Alias: -
Gene lenght: 846
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: Hepatitis B
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: EF-1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: PAAV-MCS-F
Reverse primer:
Copy number: Low
Induction:

pAAV-EF1a-M-HBsAg plasmid Description

pAAV-EF1a-M-HBsAg This is a plasmid suitable for protein expression in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-EF1a-M-HBsAg plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt cGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG 1381 AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA 1441 ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT 1501 ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC 1561 AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT GGCCCTTGCG 1621 TGCCTTGAAT TACTTCCACC TGGCTGCAGT ACGTGATTCT TGATCCCGAG CTTCGGGTTG 1681 GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG CTTAAGGAGC CCCTTCGCCT CGTGCTTGAG 1741 TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC GCGTGCGAAT CTGGTGGCAC CTTCGCGCCT 1801 GTCTCGCTGC TTTCGATAAG TCTCTAGCCA TTTAAAATTT TTGATGACCT GCTGCGACGC 1861 TTTTTTTCTG GCAAGATAGT CTTGTAAATG CGGGCCAAGA TCTGCACACT GGTATTTCGG 1921 TTTTTGGGGC CGCGGGCGGC GACGGGGCCC GTGCGTCCCA GCGCACATGT TCGGCGAGGC 1981 GGGGCCTGCG AGCGCGGCCA CCGAGAATCG GACGGGGGTA GTCTCAAGCT GGCCGGCCTG 2041 CTCTGGTGCC TGGCCTCGCG CCGCCGTGTA TCGCCCCGCC CTGGGCGGCA AGGCTGGCCC 2101 GGTCGGCACC AGTTGCGTGA GCGGAAAGAT GGCCGCTTCC CGGCCCTGCT GCAGGGAGCT 2161 CAAAATGGAG GACGCGGCGC TCGGGAGAGC GGGCGGGTGA GTCACCCACA CAAAGGAAAA 2221 GGGCCTTTCC GTCCTCAGCC GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA 2281 GGCACCTCGA TTAGTTCTCG AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT 2341 TTTATGCGAT GGAGTTTCCC CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC 2401 ACTTGATGTA ATTCTCCTTG GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA 2461 AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ggtaccgagc 2521 tcggatccac tagtaacggc cgccagtgtg ctggaattct gcagatatcc catgcagtgg 2581 aattccacaa ccttccacca aactctgcaa gatcccagag tgagaggcct gtatttccct 2641 gctggtggct ccagttcagg aacagtaaac cctgttctga ctactgcctc tcccttatcg 2701 tcaatcttct cgaggattgg ggaccctgcg ctgaacatgg agaacatcac atcaggattc 2761 ctaggacccc ttctcgtgtt acaggcgggg tttttcttgt tgacaagaat cctcacaata 2821 ccgcagagtc tagactcgtg gtggacttct ctcaattttc tagggggaac taccgtgtgt 2881 cttggccaaa attcgcagtc cccaacctcc aatcactcac caacctcttg tcctccaact 2941 tgtcctggtt atcgctggat gtgtctgcgg cgttttatca tcttcctctt catcctgctg 3001 ctatgcctca tcttcttgtt ggttcttctg gactatcaag gtatgttgcc cgtttgtcct 3061 ctaattccag gatcctcaac aaccagcacg ggaccatgcc ggacctgcat gactactgct 3121 caaggaacct ctatgtatcc ctcctgttgc tgtaccaaac cttcggacgg aaattgcacc 3181 tgtattccca tcccatcatc ctgggctttc ggaaaattcc tatgggagtg ggcctcagcc 3241 cgtttctcct ggctcagttt actagtgcca tttgttcagt ggttcgtagg gctttccccc 3301 actgtttggc tttcagttat atggatgatg tggtattggg ggccaagtct gtacagcatc 3361 ttgagtccct ttttaccgct gttaccaatt ttcttttgtc tttgggtata catttaagcg 3421 gccgcggatc ctctagagtc gacctgcaga agcttgcctc gagcagcgct gctcgagaga 3481 tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca 3541 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg 3601 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga 3661 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat 3721 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg 3781 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga 3841 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc 3901 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc 3961 ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga 4021 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc 4081 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca 4141 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg 4201 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 4261 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 4321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 4381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat 4441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 4501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc 4561 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 4621 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc 4681 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca 4741 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 4801 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga 4861 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 4921 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 4981 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 5041 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 5101 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 5161 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 5221 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 5281 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 5341 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 5401 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 5461 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 5521 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 5581 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 5641 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 5701 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 5761 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 5821 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 5881 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 5941 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 6001 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 6061 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 6121 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 6181 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 6241 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 6301 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 6361 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 6421 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 6481 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 6541 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 6601 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 6661 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 6721 tggccttttg ctcacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.