pCMV-REG1A-TM8-P2A-mCherry-Neo

pCMV-REG1A-TM8-P2A-mCherry-Neo plasmid

PVT102853

pCMV-REG1A-TM8-P2A-mCherry-Neo plasmid Information

Plasmid name: pCMV-REG1A(human)-TM8-P2A-mCherry-Neo
Alias: NM_002909.5;ICRF; P19; PSP; PSPS; PSPS1; PTP; REG
Gene lenght: 498
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-REG1A-TM8-P2A-mCherry-Neo plasmid Description

pCMV-REG1A-TM8-P2A-mCherry-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-REG1A-TM8-P2A-mCherry-Neo plasmid Sequence

1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc tagcgtttaa acttaagctt ggtaccgagc tcggatccGG CCACCatggc 781 tcagaccagc tcatacttca tgctgatctc ctgcctgatg tttctgtctc agagccaagg 841 ccaagaggcc cagacagagt tgccccaggc ccggatcagc tgcccagaag gcaccaatgc 901 ctatcgctcc tactgctact actttaatga agaccgtgag acctgggttg atgcagatct 961 ctattgccag aacatgaatt cgggcaacct ggtgtctgtg ctcacccagg ccgagggtgc 1021 ctttgtggcc tcactgatta aggagagtgg cactgatgac ttcaatgtct ggattggcct 1081 ccatgacccc aaaaagaacc gccgctggca ctggagcagt gggtccctgg tctcctacaa 1141 gtcctggggc attggagccc caagcagtgt taatcctggc tactgtgtga gcctgacctc 1201 aagcacagga ttccagaaat ggaaggatgt gccttgtgaa gacaagttct cctttgtctg 1261 caagttcaaa aacgccaccA TGGGCGGAGG CTCCGGCGGA GGCTCCGGCG GAGGCTCCat 1321 ctacatctgg gcgcccttgg ccgggacttg tggggtcctt ctcctgtcac tggttatcac 1381 cctttacgaa ttcggaagcg gagctactaa cttcagcctg ctgaagcagg ctggcgacgt 1441 ggaggagaac cctggaccta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa 1501 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 1561 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 1621 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 1681 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 1741 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 1801 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 1861 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 1921 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 1981 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 2041 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 2101 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 2161 gtacaagtaa ctcgagtcta gagggcccct taagggttcg atccctaccg gttagtaatg 2221 agtttgatat ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 2281 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 2341 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 2401 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 2461 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 2521 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 2581 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 2641 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 2701 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 2761 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 2821 cccgcctgga aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc 2881 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca 2941 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 3001 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 3061 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg 3121 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 3181 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 3241 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat 3301 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 3361 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 3421 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 3481 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga 3541 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 3601 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 3661 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3721 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 3781 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 3841 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 3901 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca 3961 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 4021 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 4081 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 4141 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc 4201 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 4261 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 4321 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 4381 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 4441 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 4501 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 4561 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 4621 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 4681 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 4741 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac 4801 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 4861 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 4921 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 4981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 5041 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 5101 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 5161 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 5221 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 5281 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 5341 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5401 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5461 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 5521 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 5581 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 5641 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 5701 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 5761 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 5821 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 5881 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 5941 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6001 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6061 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6121 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6181 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6241 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6301 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6361 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6421 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6481 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 6541 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 6601 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 6661 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 6721 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 6781 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 6841 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 6901 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 6961 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7021 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7081 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 7141 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7201 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7261 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtc gactctcagt 7321 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag 7381 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat 7441 tgcatgaaga atctgcttag g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.