pCMV-Usp29-3×Myc-Neo plasmid

pCMV-Usp29-3×Myc-Neo plasmid

PVT102831

pCMV-Usp29-3×Myc-Neo plasmid Information

Plasmid name: pCMV-Usp29(mouse)-3×Myc-Neo
Alias: NM_001290994.1;Ocat
Gene lenght: 2607
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Usp29-3×Myc-Neo plasmid Description

pCMV-Usp29-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Usp29-3×Myc-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atggctcacc taaagataaa tggtttggtc 961 cagatccgca gcacaaacag gagcaagcac accagggcat ctcagtggaa ggaagcagtc 1021 atcgaaatag tagaaagaaa acagaaagtt aatctggtgg tttctttcaa gttggaagaa 1081 aggagaaggg tgtttcagct gggtgataat gttacaggcg tagtggttag tggtgagctg 1141 ggactgtatc atctggattt aactttgaga gacgacacat ccttactcat cgacaagtta 1201 tcctctgcag atgttgaaca tttgaagtca ttcctggatt cgtctactcc atgtgagtcc 1261 cagcaaccca tggagcccat gagtagtcag gatgaccttg aaagcagtga tccattttgc 1321 ggggagcacc aggaggcagc ctgtggatcc ctgaatacca ctccagaaag tgggacacca 1381 ctgtcccgca agatgccact ttctatgtca aacaccacgg gcggccaaaa gagaggagaa 1441 aaacaaggca gaaagaggaa gacagaacca tcctcctcca gcgcagaagt gaacaaggac 1501 attcccaaag aaaatactcc tgaccaaaaa aagaaatcca ggcgatatta ttccaggaac 1561 agaggcggca aagcagagaa agctgtgacc ttaagagaac aagaaaagcg tagcaattgg 1621 aaacttgaac ctgctttcaa ttccaaatcc tatggaaggg ctaacctgga tggcactatc 1681 cttccaatcg cgacatgctc tgatgacaga gatgtatcaa tatttggact ggagatcata 1741 acacataatg gagtccaatc tcttcctgac ccttacctaa atcaactaaa acgtgaaggc 1801 ttcccaaact tgggaaatac ttgttacatg aattccattt tgcaatctgt ctttgggatt 1861 ccaacctttg caaaggactt actcactcaa ggcatcccat gggagaaagt ctcctacgat 1921 gaccttatca tgcccttaag ccagctcctt gtcttgaaag acattcggga tgtagagatc 1981 aagggggagt tactcaccag tgtaaagaag tccatttcca cagttgcaga cacattctcg 2041 ggcaatgaac agaacgatgc ccatgagttt ttaagtctgt gtttagacca gctgaaactg 2101 aacatggaaa aagtaaatgc catgtgggat actgagagga gaaacacatg tgctgggtct 2161 gctggcacca aaaggtttgt ttgtcccgtt ggagctaatt ttgaatttga gctgcatagc 2221 tccatcatct gtgaaggctg tggtgaggct actatcaaaa ccgaagtgag taattacctc 2281 tccattgacc tgcaccatgg gacaaaaaca caccctctat caattcaaaa gtcttttgat 2341 cttttcttta caccagaaaa aattgagcat aactgtgaga aatgtaagaa caagaattct 2401 gttctcaagt acacattgag gagactcccc agggtcctca ttgttcatct gaaacgctac 2461 caagttacca ctgacttgtt gccagtgaag agtgagcagc cagttgagat ttctaagtat 2521 ttaaatatat cttcccactg ccatgaaaac aggaagctac catttccctt ggctaataca 2581 tccccagatg tctctcaagg gatgatgcct gggatcttca accagtcaat gttatctaag 2641 aaggtgattt cagaatcctg tgaccccatg gttctacagg ttggatccag tgtggatgct 2701 gaaatacaaa gcttccagat aatgtatgag gatgaagatg caagtgagga gcagcagcag 2761 agaggcctgg aaagtggctc catgctggag ccagaactag tgaagacaga aaaccggata 2821 ctcagacaaa agacatcact agcaactgac tcaatgatgg gtgacggata cagcttcctt 2881 cctatgctct gtgagcctct gagcatccaa gatccaggtc ttgcagagat gggtcttcaa 2941 gaggttccag agaatccaga atttaagaac tatgagaaaa ttaacatata tggaaagtca 3001 gatggccgca ctaacactga gttgtcaaaa ctctaccaga atcatggaag tcgaatcaag 3061 ggtttgttcc taccggcatc ccttgcaagt gttagttctc aggaagaccc agagaaggac 3121 ctgagtaggt ctccagaatt gcaggaagat gacccacatt cctttgcatt tggttctgat 3181 gattccaaag atggggagat gggagatgat cttcagaatt acaggctcgt cagtgtggtc 3241 agccactttg ggagctcccc aaattcaggc cattacgtaa gtgatgtgta tgactttcaa 3301 aagcaagcct ggctccttta cagtgatgtc caagtgttcg agagttcaga tccctccatt 3361 caggagaaca ggctaaacag cggatacatc ttcttttaca tgcataatga gatttttgag 3421 gaactgttga aaaaggcatc tgagtgcaag gtccttagca catctaagga ggaaaagagg 3481 gacatagatt acttctcaac attgcttaac ggccttacat acatcctgga agaattcCTC 3541 GAGGAACAAA AACTAATATC AGAGGAAGAT TTGGAACAGA AACTAATCAG TGAAGAAGAT 3601 TTAGAGCAAA AGTTAATTTC CGAGGAGGAC TTAtgaGGGC CCGTTTAAAC CCGCTGATCA 3661 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC 3721 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG 3781 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG 3841 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG 3901 GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA 3961 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 4021 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 4081 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 4141 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 4201 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 4261 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 4321 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG 4381 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 4441 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 4501 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 4561 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT 4621 TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 4681 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 4741 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 4801 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 4861 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 4921 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 4981 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 5041 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 5101 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 5161 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 5221 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 5281 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 5341 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 5401 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 5461 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 5521 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 5581 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 5641 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 5701 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 5761 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 5821 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 5881 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 5941 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 6001 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 6061 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 6121 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 6181 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 6241 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 6301 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 6361 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 6421 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 6481 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 6541 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 6601 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 6661 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 6721 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 6781 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 6841 CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 6901 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 6961 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 7021 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 7081 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 7141 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 7201 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 7261 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 7321 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 7381 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 7441 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 7501 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 7561 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 7621 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 7681 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 7741 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 7801 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 7861 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 7921 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 7981 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 8041 ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG 8101 T

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.