pT7-TrxA-6×His-PAK1-6×His plas

pT7-TrxA-6×His-PAK1-6×His plasmid

PVT102756

pT7-TrxA-6×His-PAK1-6×His plasmid Information

Plasmid name: pT7-TrxA-6×His-PAK1(human-Toxic-opt)-6×His
Alias: NM_001376273.1;alpha-PAK; IDDMSSD; p65-PAK; PAKalpha
Gene lenght: 1635
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: TrxA-F1
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-TrxA-6×His-PAK1-6×His plasmid Description

pT7-TrxA-6×His-PAK1-6×His is a plasmid suitable for protein expression in Escherichia coli.

pT7-TrxA-6×His-PAK1-6×His plasmid Sequence

1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc 3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg 3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca 3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag 3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc 3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc 3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct 3901 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta 3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg 4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct 4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga 4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc 4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct 4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt 4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc 4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc 4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact 4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga 4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc 4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg 4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc 4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat 5101 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa 5161 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gagcgataaa 5221 attattcacc tgactgacga cagttttgac acggatgtac tcaaagcgga cggggcgatc 5281 ctcgtcgatt tctgggcaga gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat 5341 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcaaaac 5401 cctggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac 5461 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc 5521 gacgctaacc tggccggttc tggttctggc catatgcacc atcatcatca tcattcttct 5581 ggtctggtgc cacgcggttc tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag 5641 cacatggaca gcccagatct gggtaccgac gacgacgaca aggccatggc tgatatcgga 5701 tccatgtcaa ataacggcct agacattcaa gacaaacccc cagcccctcc gatgagaaat 5761 accagcacta tgattggagc cggcagcaaa gatgctggaa ccctaaacca tggttctaaa 5821 cctctgcctc caaacccaga ggagaagaaa aagaaggacc gattttaccg atccatttta 5881 cctggagata aaacaaataa aaagaaagag aaagagcggc cagagatttc tctcccttca 5941 gattttgaac acacaattca tgtcggtttt gatgctgtca caggggagtt tacgggaatg 6001 ccagagcagt gggcccgctt gcttcagaca tcaaatatca ctaagtcgga gcagaagaaa 6061 aacccgcagg ctgttctgga tgtgttggag ttttacaact cgaagaagac atccaacagc 6121 cagaaatacA TGTCATTTAC CGATAAGTCA GCAGAAGATT ATAATAGCTC AAACGCTCTG 6181 AACGTGAAGG CTGTGTCAGA GACACCAGCT GTCCCTCCTG TCAGCGAGGA CGAGGACGAC 6241 GACGACGACG ATGCAACCCC TCCCCCTGTT ATTGCGCCTC GGCCAGAGCA CACAAAGAGT 6301 GTGTACACAC GGAGCGTGAT TGAGCCGCTC CCTGTGACCC CTACAAGGGA CGTCGCTACC 6361 AGCCCAATCT CCCCTACCGA GAACAATACC ACACCTCCTG ACGCGCTCAC TCGCAACACA 6421 GAGAAGCAAA AGAAGAAACC CAAGATGTCA GATGAAGAAA TCCTGGAGAA GCTCAGAAGT 6481 ATTGTAAGCG TTGGCGATCC CAAGAAAAAA TACACCCGGT TTGAAAAGAT CGGGCAAGGC 6541 GCTTCAGGTA CTGTTTATAC TGCTATGGAT GTCGCGACTG GCCAAGAAGT GGCGATAAAA 6601 CAGATGAACC TCCAGCAGCA GCCGAAGAAA GAACTCATCA TAAACGAGAT TCTGGTGATG 6661 AGGGAAAACA AGAATCCAAA TATAGTTAAC TACCTGGACA GTTATCTTGT GGGCGATGAG 6721 TTGTGGGTCG TGATGGAGTA TTTGGCCGGA GGGTCTCTGA CTGATGTTGT TACCGAAACC 6781 TGTATGGACG AGGGCCAGAT TGCTGCTGTA TGTCGGGAAT GCCTCCAAGC TCTGGAGTTT 6841 CTGCATAGTA ACCAGGTAAT CCATCGCGAC ATTAAGTCAG ATAACATACT GTTGGGGATG 6901 GATGGCTCAG TTAAGCTGAC AGACTTCGGC TTCTGTGCAC AGATCACTCC TGAACAGAGC 6961 AAAAGGAGTA CAATGGTCGG TACCCCCTAC TGGATGGCAC CAGAGGTTGT GACACGGAAA 7021 GCCTATGGCC CCAAAGTCGA CATCTGGAGT CTCGGAATAA TGGCCATTGA GATGATCGAG 7081 GGGGAACCAC CCTACCTGAA TGAGAACCCT CTGAGAGCAC TGTATCTTAT CGCCACTAAC 7141 GGAACTCCAG AGCTCCAGAA CCCTGAAAAA CTCAGTGCCA TTTTCCGCGA CTTTCTGAAC 7201 CGCTGTCTGG AGATGGATGT GGAAAAGCGC GGGTCCGCTA AAGAGCTGCT GCAGCATCAG 7261 TTTCTGAAGA TAGCTAAGCC CCTGTCATCT CTGACGCCCT TGATCGCAGC CGCTAAGGAG 7321 GCTACAAAAA ACAATCATct cgagcaccac caccaccacc actgagatcc ggctgctaac 7381 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc 7441 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga 7501 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.