pCMV-NBR1-3×HA-Neo plasmid

pCMV-NBR1-3×HA-Neo plasmid

PVT102639

pCMV-NBR1-3×HA-Neo plasmid Information

Plasmid name: pCMV-NBR1(human)-3×HA-Neo
Alias: NM_005899.5;1A1-3B; IAI3B; M17S2; MIG19
Gene lenght: 2898
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-NBR1-3×HA-Neo plasmid Description

pCMV-NBR1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-NBR1-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atggaaccac aggttactct aaatgtgact 961 tttaaaaatg aaattcaaag ctttctggtt tctgatccag aaaatacaac ttgggctgat 1021 atcgaagcta tggtaaaagt ttcatttgat ctgaatacta ttcaaataaa atacctggat 1081 gaggaaaatg aagaggtatc catcaacagt caaggagaat atgaagaagc gcttaagatg 1141 gcagttaaac agggaaacca actgcagatg caagtccacg aagggcacca tgtcgttgat 1201 gaagccccac ccccagttgt aggagcaaaa cgactagctg ccagggcagg gaagaagcca 1261 cttgcacatt actcttcact ggtgagagtc ttgggatcag acatgaagac cccagaggat 1321 cctgcagtgc agtcgtttcc acttgttcca tgtgacacag accagcctca agacaagccc 1381 ccagactggt tcacaagcta cctggagacg ttcagagaac aagtggttaa cgaaacggtt 1441 gagaagcttg aacagaaatt acatgaaaag cttgtcctcc agaacccatc cttgggttct 1501 tgtccctcag aagtctcaat gcctacttca gaagaaacat tgtttttgcc agaaaaccag 1561 ttcagctggc atattgcttg caacaactgc caaagaagga ttgttggtgt ccgctaccag 1621 tgtagcctat gcccatccta caatatctgt gaagattgtg aagcagggcc atatggccat 1681 gacactaacc acgtcctgct gaagttgcgg agacctgttg tgggctcctc tgaaccgttc 1741 tgtcactcaa agtactctac tcctcgtctt cctgctgctc tggaacaagt caggctccag 1801 aaacaggttg ataagaactt tcttaaagca gaaaagcaaa ggttgcgagc tgagaagaaa 1861 caacgtaaag cagaggtcaa ggaacttaaa aagcagctta aactccatag gaaaattcac 1921 ctgtggaatt caatccatgg actccagagc cccaagtctc ctttaggccg acctgagagc 1981 ttgctccagt ctaataccct gatgctccct ttgcagccct gtacctccgt tatgccaatg 2041 ctcagtgcag catttgtgga tgagaatttg cctgatggga ctcaccttca gccaggaacc 2101 aagtttatca aacactggag gatgaaaaat acaggaaatg taaagtggag tgcagacaca 2161 aagctcaagt tcatgtgggg aaacctgact ttggcttcca cagaaaagaa ggatgttttg 2221 gttccctgcc tcaaggccgg ccatgtggga gttgtatctg tggagttcat tgccccagcc 2281 ttggagggaa cgtatacttc ccattggcgt ctttctcaca aaggccagca atttgggcct 2341 cgggtctggt gcagtatcat agtagatcct ttcccctccg aagagagccc tgataacatt 2401 gaaaagggca tgatcagctc aagcaaaact gatgatctca cctgccagca agaggaaact 2461 tttcttctgg ctaaagaaga aagacagctt ggtgaagtga ctgagcagac agaagggaca 2521 gcagcctgca tcccacagaa ggcaaaaaat gttgccagtg agagggagct ctacatccca 2581 tctgtggatc ttctgactgc ccaggacctg ctgtcctttg agctgttgga tataaacatt 2641 gttcaagagt tggagagagt gccccacaac acccctgtgg atgtgactcc ctgcatgtct 2701 cctctgccac atgacagtcc tttaatagag aagccaggct tggggcagat agaggaagag 2761 aatgaagggg caggatttaa agcacttcct gattctatgg tgtcagtaaa gaggaaggct 2821 gagaacattg cttctgtgga ggaagcagaa gaagacctga gtgggaccca gtttgtgtgt 2881 gagacagtaa tccgatccct taccttggat gctgccccag accacaaccc tccttgcaga 2941 cagaagtcct tgcagatgac atttgccttg cctgaaggac cacttggaaa tgagaaggag 3001 gagattatcc atatcgctga ggaagaagct gtcatggagg aggaggagga tgaggaggat 3061 gaggaggagg aggatgagct caaagatgaa gttcaaagtc agtcctctgc ttcctcagag 3121 gattacatca tcatcctgcc tgagtgcttt gataccagcc gccccctggg ggattctatg 3181 tacagctctg cgctctcaca gccaggcctg gagcgaggtg ctgaaggcaa gcctggggtt 3241 gaggctgggc aggaaccagc tgaggctggg gaaagactcc ctggagggga gaaccagcca 3301 caggagcaca gcataagtga catcctcacg acctcacaga ctctggaaac agtgccccta 3361 atcccagagg tagtggagct tccaccgtca ctgcccagga gctctccttg tgtacatcat 3421 catggttccc caggagtgga tttaccagtt accataccag aagtttcttc agtccctgat 3481 cagatcagag gagagcccag aggctcatca ggacttgtaa acagcagaca gaagagctat 3541 gaccactcaa ggcaccatca tgggagcagc attgctggag gactggtgaa gggggctttg 3601 tctgttgctg cctctgcata caaggccctg tttgctgggc caccagtcac tgcacagcca 3661 ataatttctg aagatcagac agcagccctg atggcccatc tctttgaaat gggattctgt 3721 gacaggcagc tgaacctacg gctgctgaag aaacacaatt acaatatcct gcaggttgtg 3781 acagaacttc ttcagttaaa caacaacgac tggtacagcc aacgctatCT CGAGTACCCA 3841 TACGACGTAC CTGATTATGC AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT 3901 TATGATGTCC CAGACTACGC AtgaGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG 3961 CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA 4021 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT 4081 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA 4141 GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC 4201 AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 4261 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 4321 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 4381 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 4441 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 4501 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 4561 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 4621 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG 4681 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 4741 AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 4801 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA 4861 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG 4921 AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG 4981 GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG 5041 AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG 5101 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG 5161 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC 5221 TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA 5281 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC 5341 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG 5401 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT 5461 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG 5521 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA 5581 GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT 5641 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG 5701 GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG 5761 GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC 5821 GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT 5881 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA 5941 TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG 6001 GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA 6061 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 6121 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG 6181 CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 6241 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 6301 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 6361 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 6421 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 6481 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 6541 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 6601 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 6661 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 6721 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 6781 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 6841 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 6901 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 6961 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 7021 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 7081 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 7141 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 7201 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 7261 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 7321 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 7381 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 7441 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 7501 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 7561 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 7621 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 7681 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 7741 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 7801 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 7861 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 7921 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 7981 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 8041 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 8101 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 8161 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 8221 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 8281 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 8341 AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.