pLV3-CMV-Serpine1-6×His-EF1a-m

pLV3-CMV-Serpine1-6×His-EF1a-mCherry-Neo plasmid

PVT102627

pLV3-CMV-Serpine1-6×His-EF1a-mCherry-Neo plasmid Information

Plasmid name: pLV3-CMV-Serpine1(mouse)-6×His-EF1a-mCherry-Neo
Alias: NM_008871.2;PAI-1; PAI1; Planh1
Gene lenght: 1206
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Serpine1-6×His-EF1a-mCherry-Neo plasmid Description

pLV3-CMV-Serpine1-6×His-EF1a-mCherry-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Serpine1-6×His-EF1a-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgcagat gtcttcagcc cttgcttgcc tcatcctggg 2341 cctggttctg gtctctggga aagggttcac tttacccctc cgagaatccc acacagccca 2401 tcaggccacc gacttcggag taaaagtgtt tcagcaggtg gtccaggcct ccaaagaccg 2461 gaatgtggtc ttctctccct atggcgtgtc ctcggtgctg gctatgctgc agatgaccac 2521 agcggggaaa acccggcggc agatccaaga tgctatggga ttcaaagtca atgagaaggg 2581 cacagctcat gccctccgcc agctctccaa ggagctcatg gggccgtgga acaagaatga 2641 gatcagtact gcggatgcca tctttgtcca gcgggaccta gagctggtcc agggcttcat 2701 gccccacttc ttcaagctct tccagactat ggtgaaacag gtggacttct cagaagtgga 2761 aagagccaga tttatcatca atgactgggt ggaaaggcat accaaaggta tgatcagtga 2821 cttactggcc aagggggctg tagacgagct gacacgcctg gtgctggtga atgccctcta 2881 cttcagtggc caatggaaga ctcctttctt agaggccagc acccaccagc gcctcttcca 2941 caagtctgat ggcagcaccg tctctgtgcc catgatggct cagagcaaca agttcaacta 3001 cactgagttc accacccccg atgggctcga gtatgacgtt gtggaactgc cctaccaggg 3061 cgacaccctc agcatgttca tcgctgcacc ctttgagaaa gatgtgcacc tctccgccct 3121 caccaacatc ttggatgctg aactcatcag acaatggaag ggcaacatga ccaggctgcc 3181 ccgcctcctc atcctgccta agttctctct ggagactgaa gtggacctca gagggcccct 3241 ggagaagttg ggcatgcctg acatgtttag tgcaaccctg gccgacttca caagtctttc 3301 cgaccaagag cagctctctg tagcacaggc actgcaaaag gtcaggatcg aggtaaacga 3361 gagcggcaca gtggcgtctt cctccacagc ctttgtcatc tcagcccgca tggcccccac 3421 ggagatggtt atagaccgat cctttctctt tgtggttcgg cacaacccga cagagacaat 3481 cctcttcatg ggccaagtga tggagcctgg atccatgcat catcaccatc accatTAgcg 3541 gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 3601 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg 3661 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 3721 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 3781 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac 3841 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc 3901 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc 3961 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct 4021 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg 4081 accggcgcct acgctagacg ccaccatggt gagcaagggc gaggaggata acatggccat 4141 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 4201 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 4261 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 4321 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 4381 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 4441 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 4501 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc 4561 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct 4621 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt 4681 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 4741 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 4801 cgagctgtac aagggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga 4861 ggagaatccc ggccctGCCA CCatggctga acaagatgga ttgcacgcag gttctccggc 4921 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 4981 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 5041 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 5101 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 5161 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 5221 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 5281 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 5341 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 5401 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 5461 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 5521 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 5581 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 5641 catcgccttc tatcgccttc ttgacgagtt cttctgaGTC GACAATCAAC CTCTGGATTA 5701 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 5761 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 5821 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 5881 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 5941 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 6001 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 6061 CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG 6121 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 6181 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 6241 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCggtacctt taagaccaat gacttacaag 6301 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 6361 tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 6421 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 6481 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 6541 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 6601 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 6661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 6721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 6781 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 6841 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 6901 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat 6961 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 7021 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 7081 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7141 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7201 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7261 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 7441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 7501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 7561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 7621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 7681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 7741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 7801 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 7861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 7921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 7981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 8041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 8101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 8161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 8221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 8281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 8341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 8401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 8461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 8521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 8581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 8641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 8701 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 8761 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 8821 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 8881 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 8941 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 9001 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 9061 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 9121 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 9181 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 9241 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 9301 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 9361 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 9421 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 9481 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 9541 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.