pCMV-3×Myc-NEDD8-Neo plasmid

pCMV-3×Myc-NEDD8-Neo plasmid

PVT102371

pCMV-3×Myc-NEDD8-Neo plasmid Information

Plasmid name: pCMV-3×Myc-NEDD8(human)-Neo
Alias: NM_006156.3;NEDD-8;
Gene lenght: 243
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×Myc-NEDD8-Neo plasmid Description

pCMV-3×Myc-NEDD8-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×Myc-NEDD8-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGGAA CAAAAACTAA TATCAGAGGA AGATTTGGAA 961 CAGAAACTAA TCAGTGAAGA AGATTTAGAG CAAAAGTTAA TTTCCGAGGA GGACTTATCA 1021 GCGGAAAAGC TTatgctaat taaagtgaag acgctgaccg gaaaggagat tgagattgac 1081 attgaaccta cagacaaggt ggagcgaatc aaggagcgtg tggaggagaa agagggaatc 1141 cccccacaac agcagaggct catctacagt ggcaagcaga tgaatgatga gaagacagca 1201 gctgattaca agattttagg tggttcagtc cttcacctgg tgttggctct gagaggagga 1261 ggtggtctta ggcagtagaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta 1321 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 1381 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 1441 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 1501 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 1561 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc 1621 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 1681 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 1741 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 1801 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 1861 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 1921 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 1981 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 2041 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 2101 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 2161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 2221 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 2281 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 2341 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 2401 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 2461 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 2521 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 2581 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 2641 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 2701 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 2761 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 2821 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 2881 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 2941 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 3001 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 3061 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 3121 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 3181 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 3241 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 3301 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 3361 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 3421 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 3481 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 3541 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 3601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 3661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 3721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 3781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 3841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 3901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 3961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 4021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 4081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 4141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 4201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 4261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 4321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 4381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 4441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 4501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag 4561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 4741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 5041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 5101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 5161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 5221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 5281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 5341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 5401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 5461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 5521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 5581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 5641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 5701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.