pLV3-CMV-GAN-3×FLAG-P2A-EGFP p

pLV3-CMV-GAN-3×FLAG-P2A-EGFP plasmid

PVT102213

pLV3-CMV-GAN-3×FLAG-P2A-EGFP plasmid Information

Plasmid name: pLV3-CMV-GAN(human)-3×FLAG-P2A-EGFP
Alias: NM_022041.4;GAN1; GIG; KLHL16
Gene lenght: 1791
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GAN-3×FLAG-P2A-EGFP plasmid Description

pLV3-CMV-GAN-3×FLAG-P2A-EGFP It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-GAN-3×FLAG-P2A-EGFP plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1981 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 2041 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2101 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2161 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2221 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2281 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2341 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2401 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2461 ccgctagcgc caccatggct gagggcagtg ccgtgtctga ccctcagcac gccgcgcgtc 2521 tgctgcgagc gctcagctct ttccgcgagg agtctcgctt ctgcgacgcg cacctggtcc 2581 tcgacgggga ggagatcccg gtgcagaaga acatcctggc ggcggccagc ccgtacatca 2641 ggacaaagtt aaactataat cctccaaaag atgatggatc aacttataag attgaacttg 2701 aagggatatc ggtaatggtt atgagagaga tcctggatta catcttcagt gggcagatca 2761 ggctaaatga agatacaatc caagatgttg ttcaggcagc tgacctgctg ctactgacgg 2821 accttaaaac cctgtgctgt gagtttttgg aaggctgcat tgctgctgag aactgtattg 2881 gtatccgtga ctttgcacta cattactgcc tccatcacgt tcattacctt gccacagaat 2941 acctggagac tcatttccga gacgtcagca gcacggaaga attcttagag ctgagtcctc 3001 aaaagcttaa agaagtgatt tctcttgaga agttaaacgt tggcaatgaa agatatgtct 3061 ttgaagcagt aattcgatgg atagcacatg atacagaaat aagaaaggtc cacatgaagg 3121 atgttatgtc agctctgtgg gtttcagggt tggactccag ttatttacgg gaacagatgc 3181 tgaatgaacc attagtacga gaaattgtca aagagtgtag caatataccg ctcagccagc 3241 cgcagcaagg ggaggcgatg ctggccaact tcaaaccccg gggctactct gagtgcatcg 3301 tgactgttgg tggagaagag agagtttcac ggaaacccac agcagcgatg cgatgcatgt 3361 gccctctcta tgaccctaac aggcagcttt ggatcgaact ggccccttta agcatgccga 3421 gaattaacca tggagttctc tcagcagaag gatttttgtt tgtattcggg ggccaagatg 3481 aaaataagca gactcttagc tcaggagaaa agtatgatcc agatgcaaat acatggacag 3541 cattgccacc tatgaacgag gcaagacata acttcggaat tgtggagata gatgggatgc 3601 tgtacatttt gggaggagag gatggtgaaa aggagctgat ttccatggag tgttacgata 3661 tttattctaa aacctggaca aagcaacctg atttgaccat ggtcagaaag atcggctgct 3721 atgcagctat gaaaaagaaa atctacgcca tgggtggagg ctcctacgga aagctttttg 3781 agtctgtaga gtgttatgat cccaggaccc agcagtggac tgccatatgt ccactaaaag 3841 agaggaggtt tggagcggtg gcctgtggag ttgctatgga gctgtatgtg tttgggggag 3901 tccgaagtcg tgaggacgcc cagggtagcg agatggtaac ttgcaagtcc gagttctacc 3961 atgatgagtt taaaaggtgg atctatctta acgaccagaa tttatgcatc cccgccagtt 4021 cctcttttgt ttatggagct gtacctatag gagccagtat ttatgttatt ggagatcttg 4081 atacaggtac caattacgac tacgtgcgtg agtttaaaag aagcacagga acctggcacc 4141 acactaaacc actccttcca tccgaccttc gccgtacagg atgtgcagcc ttacgcattg 4201 cgaattgcaa gcttttccgc ctgcagcttc agcaaggctt attccgtatt cgtgttcatt 4261 cccctgtcga cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA 4321 AGGATGACGA TGACAAGGGA AGCGGAGCCA CTAACTTCTC CCTGTTGAAA CAAGCAGGGG 4381 ATGTCGAAGA GAATCCCGGG CCAgaattcG CCACCatggt gagcaagggc gaggagctgt 4441 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 4501 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 4561 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 4621 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 4681 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 4741 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 4801 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 4861 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 4921 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 4981 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 5041 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 5101 ggatcactct cggcatggac gagctgtaca agtaaGGATC Caatcaacct ctggattaca 5161 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 5221 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 5281 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 5341 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 5401 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 5461 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 5521 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 5581 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 5641 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga 5701 gtcggatctc cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg 5761 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 5821 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 5881 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 5941 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 6001 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 6061 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 6121 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 6181 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 6241 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 6301 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 6361 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 6421 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 6481 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 6541 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 6601 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 6661 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 6721 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 6781 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 6841 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 6901 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 6961 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 7021 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 7081 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 7141 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 7201 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7261 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7321 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 7381 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 7441 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 7501 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 7561 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 7621 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 7681 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 7741 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 7801 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 7861 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 7921 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7981 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 8041 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 8101 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 8161 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8221 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8281 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 8341 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 8401 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 8461 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 8521 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 8581 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 8641 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 8701 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 8761 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 8821 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 8881 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 8941 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 9001 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.