pLV3-U6-Ngfr-shRNA1-CopGFP-Pur

pLV3-U6-Ngfr-shRNA1-CopGFP-Puro plasmid

PVT102133

pLV3-U6-Ngfr-shRNA1-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-Ngfr(mouse)-shRNA1-CopGFP-Puro
Alias: GTGGGCCTTGTGGCCTATATT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-Ngfr-shRNA1-CopGFP-Puro plasmid Description

pLV3-U6-Ngfr-shRNA1-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-Ngfr-shRNA1-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagagGAGc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtATCG 1801 GTtaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat 1921 tttatcgata ctagtGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC 1981 AAGGCTGTTA GAGAGATAAT TAGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA 2041 AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT 2101 AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2161 ATCTTGTGGA AAGGACTCAT Cgctagcgaa ttcgGTGGGC CTTGTGGCCT ATATTctcga 2221 gAATATAGGC CACAAGGCCC ACttttttgg atccgcggcc gcGaaggatc tgcgatcgct 2281 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2341 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2401 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2461 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag 2521 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt 2581 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg 2641 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta 2701 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt 2761 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca 2821 ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca 2881 ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc 2941 gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga 3001 gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc 3061 ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg 3121 acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg 3181 acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca 3241 tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca 3301 gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc 3361 acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg 3421 ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc 3481 acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg 3541 tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa 3601 catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc 3661 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 3721 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 3781 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 3841 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 3901 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 3961 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 4021 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 4081 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 4141 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 4201 cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg 4261 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 4321 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 4381 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 4441 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 4501 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 4561 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 4621 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg 4681 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 4741 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct 4801 ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta 4861 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga 4921 agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 4981 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 5041 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 5101 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 5161 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 5221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 5281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta 5341 tcccgcccct aactccgCCC ATCCCGCCCC TAACTCCGcc cagttccgcc cattctccgc 5401 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 5461 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gaccaaattc 5521 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 5581 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 5641 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 5701 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 5761 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 5821 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 5881 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 5941 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 6001 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 6061 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 6121 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 6181 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 6241 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 6301 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 6361 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 6421 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 6481 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 6541 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 6601 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 6661 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 6721 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 6781 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 6841 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 6901 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 6961 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 7021 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 7081 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 7141 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 7201 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 7261 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 7321 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 7381 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 7441 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 7501 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 7561 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 7621 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 7681 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 7741 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 7801 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 7861 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 7921 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 7981 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 8041 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 8101 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.