pLV3-CMV-KLHL1-3×FLAG-P2A-EGFP

pLV3-CMV-KLHL1-3×FLAG-P2A-EGFP plasmid

PVT102125

pLV3-CMV-KLHL1-3×FLAG-P2A-EGFP plasmid Information

Plasmid name: pLV3-CMV-KLHL1(human)-3×FLAG-P2A-EGFP
Alias: NM_020866.3;MRP2
Gene lenght: 2244
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KLHL1-3×FLAG-P2A-EGFP plasmid Description

pLV3-CMV-KLHL1-3×FLAG-P2A-EGFP It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-KLHL1-3×FLAG-P2A-EGFP plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1981 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 2041 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2101 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2161 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2221 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2281 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2341 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2401 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2461 ccgctagcgc caccatgtca ggctctgggc gaaaagactt cgatgtgaag cacattctgc 2521 gactccgctg gaaactcttc agccacccgt ctccttccac cggcggcccg gcggggggag 2581 gctgcctgca acaggacggc agtggcagct ttgagcactg gggacccagc cagagtcgcc 2641 tgctcaaaag ccaagagaga agcggtgtga gcactttctg gaagaagcct tcctcctctt 2701 cctcttcatc gtcctccccg tcctcttcct cctcttcctt caatccgctg aatggcaccc 2761 tgcttccagt tgccacgagg ctgcagcaag gggctcctgg gcagggcact cagcagccag 2821 ccaggactct cttctacgtg gagtcactag aggaggaggt ggtgccaggc atggactttc 2881 ctggaccaca cgaaaaaggg ctggttctgc aagagctcaa agtggagcca gacaactcta 2941 gccaggcaac aggtgaagga tgtggacaca ggctgtcatc aaccggccat tcaatgacac 3001 ctcaaagtga tttggactcc agtagctctg aagaattcta tcaagctgtt catcatgctg 3061 agcaaacctt cagaaagatg gaaagttatt tgaagcagca gcaactttgt gatgttatcc 3121 tgattgttgg gaaccgaaag atacctgcac ataggcttgt tctgagttca gtctccgact 3181 attttgcggc catgtttaca agtgatgttt gtgaagccaa gcaagaggag atcaaaatgg 3241 aaggcataga ccccaatgct ctctgggacc ttgtccaatt tgcatataca ggctgcttgg 3301 aattaaaaga ggacaccatt gagaaccttc ttgctgcagc gtgccttctt cagcttccac 3361 aggtggtgga agtgtgctgc cacttcctca tgaagctttt gcatccatct aactgtttag 3421 gaattcgagc cttcgcagat gctcaaggat gcattgagtt aatgaaggtg gcccacagct 3481 acacaatgga aaacataatg gaagttatca gaaatcaaga gtttttactc cttccagctg 3541 aggagctcca taaactactg gccagtgatg atgtcaatgt tcctgatgaa gaaaccatct 3601 tccatgcatt gatgatgtgg gtcaagtatg acatgcagag tagatgcaat gacctgagca 3661 tgcttcttgc ctttataaga ctgccactgc ttccaccaca gatattggct gacctagaaa 3721 atcatgcatt atttaagaat gatctggaat gtcaaaagct gattctagaa gcaatgaaat 3781 accatctatt gccagaaaga agaactttaa tgcaaagtcc gagaactaaa cccagaaaat 3841 ctacagtcgg aactttgtat gctgtaggag gaatggataa caacaaagga gctacaacta 3901 tagagaaata tgatctgaga acaaatctgt ggatccaggc agggatgatg aatggcagaa 3961 ggctgcagtt tggtgtggct gttattgatg acaaactctt tgtaattgga ggtcgagatg 4021 gcttaaagac attgaacact gttgaatgtt acaatcccaa aaccaagaca tggactgtct 4081 taccaccaat gtcaacacac agacatggtc taggtgtaac agtacttgaa ggccctattt 4141 atgctgtggg aggccatgat ggctggagct atctgaatac agtggaaagg tgggatccac 4201 agagtcaaca atggacattt gtagccagta tgtcaattgc tcggagcaca gttggtgtag 4261 cagcattgaa tggcaagttg tattcagttg gaggtcgtga tggaagttcc tgtttgagtt 4321 caatggaata ttatgatcct catacaaata agtggaacat gtgtgctccc atgtgtaaga 4381 ggagaggggg tgtcggagtg gccacatgtg acggttttct ttatgcagta ggaggtcatg 4441 atgctcctgc ttcaaatcac tgttcccggc tactggatta tgtagaaaga tatgatccca 4501 aaacagacac ttggaccatg gtggctcctt tgagtatgcc cagagatgct gttggggtct 4561 gtctccttgg tgacagatta tatgctgttg gtggctatga tggacagaca tacctcaaca 4621 ctatggaatc ctatgaccca caaactaatg agtggacaca gatggcttcc ttgaatattg 4681 ggagagcagg tgcctgtgtg gtagtcatca agcaacctgt cgacGACTAC AAAGACCATG 4741 ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG GGAAGCGGAG 4801 CCACTAACTT CTCCCTGTTG AAACAAGCAG GGGATGTCGA AGAGAATCCC GGGCCAgaat 4861 tcGCCACCat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 4921 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 4981 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 5041 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 5101 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 5161 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 5221 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 5281 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 5341 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 5401 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 5461 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 5521 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 5581 acaagtaaGG ATCCaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 5641 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 5701 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 5761 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 5821 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 5881 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 5941 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 6001 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 6061 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 6121 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc 6181 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 6241 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg 6301 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 6361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 6421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 6481 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 6541 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 6601 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 6661 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 6721 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 6781 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga 6841 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg 6901 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 6961 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 7021 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 7081 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7141 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7201 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7261 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7681 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7741 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7801 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7861 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7921 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7981 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8041 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8101 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8161 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8221 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8281 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8341 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8401 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8461 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8521 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8581 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8641 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8701 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8761 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8821 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8881 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8941 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 9001 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 9061 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 9121 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 9181 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 9241 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 9301 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 9361 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 9421 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9481 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.