pLV3-CMV-SEC24C-3×HA-EF1a-mChe

pLV3-CMV-SEC24C-3×HA-EF1a-mCherry-Puro plasmid

PVT102101

pLV3-CMV-SEC24C-3×HA-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-SEC24C(human)-3×HA-EF1a-mCherry-Puro
Alias: NM_004922.4
Gene lenght: 3282
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-SEC24C-3×HA-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-SEC24C-3×HA-EF1a-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SEC24C-3×HA-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgaacgt caaccagtca gttccacctg tgccaccatt 2341 tgggcagccc cagcccatct acccagggta tcatcagtcc agctatggtg ggcaatcagg 2401 gtccacagcc cccgccattc cctatggagc ctacaatggc ccagtaccag gctatcagca 2461 aacacctccc caaggtatgt caagagcccc accttcctcg ggggcacctc cagcctcaac 2521 agcacaggct ccttgtggcc aggctgcata tggccagttt ggccaaggag atgtacagaa 2581 tgggccaagc tccactgttc agatgcaaag gctgcctggg tctcagccat ttgggtcccc 2641 attggcccct gtgggcaacc agccacctgt gcttcagccc tatggccctc ccccgacaag 2701 tgcacaggtg gctacgcagc tgtctggaat gcagatcagc ggtgctgtgg ccccagcccc 2761 tccttcttca gggctgggct ttggcccacc aacatcgctg gcttcagcct caggaagttt 2821 ccctaactct ggtctgtatg gctcctatcc tcagggccag gctcctcccc ttagccaggc 2881 ccaaggtcat cctgggatcc agactcccca gcgatctgcc ccatcacagg cctccagctt 2941 cacaccccca gcttcagggg gtcctcggct gccttcgatg actggtccac tcctgcctgg 3001 acagagtttt ggagggccct cagtgagcca gcccaaccat gtgtcttcac ctcctcaagc 3061 tctgccccct ggcacccaga tgactgggcc cctgggacca ctgccaccta tgcactcccc 3121 gcagcagcca ggctatcagc cccaacaaaa tggttccttc ggaccagccc ggggccctca 3181 gtctaattat ggaggcccct acccagcagc acccaccttt ggcagtcagc ctgggcctcc 3241 tcagccactg cctcctaagc gcctggaccc tgatgccatc ccaagcccta ttcaggtcat 3301 tgaagatgac aggaacaacc ggggtacaga gccatttgtt actggagtac ggggccaggt 3361 gccaccctta gtcactacca acttcctggt gaaagaccaa gggaatgcaa gtccccgata 3421 catccgatgt acatcctata atatcccttg cacatctgac atggctaagc aggctcaggt 3481 gcccctggca gcagtcatca aaccgctggc aaggctgccc ccagaggagg cttcaccgta 3541 tgttgtggac catggggaat ctggcccttt gcgctgcaac cgctgcaaag catacatgtg 3601 tcccttcatg cagttcattg aaggagggag gcgtttccag tgctgttttt gcagctgtat 3661 caatgatgtt cccccccagt attttcagca cctggatcat accggcaaac gtgtggatgc 3721 ttatgaccgc cctgagctat ccctgggctc ttatgaattc ttggccactg tagattactg 3781 caagaacaat aagttcccca gccctcctgc ctttatcttc atgattgacg tctcctacaa 3841 tgccatcagg actggtcttg ttaggctcct ctgtgaggag ctcaagtcac tgttagactt 3901 tctacctagg gagggtgggg cagaagagtc agcaatccgc gttggctttg tcacctacaa 3961 taaggtgctc cacttctata atgtgaagag ctcattggcc cagccacaga tgatggttgt 4021 gtctgatgtg gctgacatgt ttgtgccact gctggatggc ttcctggtca acgtcaatga 4081 gtctcgggca gttatcacca gcttattgga tcagattcca gaaatgtttg cagacacaag 4141 ggaaacagag acagtatttg taccagttat ccaggctgga atggaggctc tgaaggctgc 4201 tgagtgtgca gggaagctct ttctattcca tacatccctg cccattgcag aggccccagg 4261 gaaactgaag aacagagatg acaggaagct gatcaataca gacaaggaga agactctgtt 4321 ccagcctcag acaggtgcct atcagaccct ggccaaagag tgtgtggccc aaggctgctg 4381 tgtagatctc tttctcttcc ctaaccagta tgtggatgtg gccacactct ctgttgtgcc 4441 ccagctcact ggtggctctg tctacaaata tgcttccttt caggtggaga acgaccagga 4501 gcggttcctg agtgacctgc gtcgtgatgt ccagaaggtt gttggctttg atgctgtgat 4561 gcgggtccgg acaagcactg gtatccgtgc tgtagatttc tttggagctt tctacatgag 4621 caacacgaca gatgtggagc tggctgggct agatggggac aaaacagtga ctgtggagtt 4681 caagcatgac gatcggctca atgaagagag cggagctctc ctgcagtgtg ccctgcttta 4741 caccagctgt gcagggcagc gtcggctccg catccataat ctggccctga actgctgcac 4801 ccagctggct gatctatatc gaaactgtga gactgacacg ctcatcaact acatggccaa 4861 gtttgcatat cggggagtcc tgaatagccc tgtgaaggct gttcgtgaca cgctcatcac 4921 ccagtgtgcc cagatcctgg cctgttacag aaagaactgt gctagcccct cctctgcagg 4981 acagttgatc cttcctgagt gcatgaagct actcccagtt tacctgaact gtgtgttgaa 5041 gagtgatgtc ctgcagcctg gagctgaagt cactactgat gaccgtgcct atgtccgaca 5101 gctagttacc tccatggatg tgactgagac caatgtcttc ttctaccctc ggctcttacc 5161 tttgacaaag tctcccgttg agagtactac cgaaccacca gcagttcgag cctctgaaga 5221 gcgtctaagc aatggggata tatatttact ggagaatggg ctcaacctct tcctctgggt 5281 gggagcaagc gtccaacagg gtgttgtcca gagccttttc agcgtctcct ccttcagtca 5341 gatcaccagt ggtttgagtg ttctgccagt tctggataat ccactgtcca agaaggttcg 5401 aggcctcatt gatagcttac gggcacagag atcccggtac atgaagctta ccgtggtgaa 5461 acaggaagac aagatggaga tgctgttcaa gcacttcctg gtggaagaca agagtctgag 5521 tgggggagca tcttatgtgg actttctctg tcatatgcac aaggagattc ggcagctact 5581 gagcggatcc TACCCATACG ACGTACCTGA TTATGCAACT TATCCTTATG ATGTACCTGA 5641 TTATGCTAGC TATCCTTATG ATGTCCCAGA CTACGCATAG gcggccgcGa aggatctgcg 5701 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 5761 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa 5821 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 5881 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc 5941 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac 6001 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg 6061 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc 6121 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct 6181 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag 6241 acgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 6301 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 6361 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 6421 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 6481 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 6541 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 6601 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 6661 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 6721 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 6781 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 6841 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 6901 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat 6961 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta 7021 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 7081 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 7141 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 7201 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 7261 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 7321 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 7381 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 7441 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 7501 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 7561 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 7621 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 7681 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 7741 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 7801 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 7861 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 7921 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 7981 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 8041 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 8101 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc 8161 gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa 8221 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 8281 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 8341 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 8401 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 8461 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 8521 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 8581 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8641 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8701 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 8761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 8821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 8881 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 8941 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 9001 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 9061 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 9121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 9181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 9241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 9301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 9361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 9421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 9481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 9541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 9601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 9661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 9721 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 9781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 9841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 9901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 9961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 10021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 10081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 10141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 10201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 10261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 10321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 10381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 10441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 10501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 10561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 10621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 10681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 10741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 10801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 10861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 10921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 10981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 11041 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 11101 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 11161 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 11221 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 11281 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 11341 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 11401 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 11461 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 11521 gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.