pLenti_PE7-2A-BSD plasmid

pLenti_PE7-2A-BSD plasmid

PVT99963

pLenti_PE7-2A-BSD plasmid Information

Plasmid name: pLenti_PE7-2A-BSD
Alias: Plasmid #234073
Gene lenght: 7071bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species:
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α-F3
Reverse primer:
Copy number: High
Induction:

pLenti_PE7-2A-BSD plasmid Description

pLenti_PE7-2A-BSD It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLenti_PE7-2A-BSD plasmid Sequence

1 CGCGTTGACA TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC 61 ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC 121 CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA 181 TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG 241 TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC 301 CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT 361 ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG 421 GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT 481 TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA 541 CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT 601 ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC 661 CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG 721 TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT 781 AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC 841 GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA 901 CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA 961 TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA 1021 AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA 1081 ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT 1141 CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT 1201 GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG 1261 AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGC TGATCTTCAG ACCTGGAGGA 1321 GGAGATATGA GGGACAATTG GAGAAGTGAA TTATATAAAT ATAAAGTAGT AAAAATTGAA 1381 CCATTAGGAG TAGCACCCAC CAAGGCAAAG AGAAGAGTGG TGCAGAGAGA AAAAAGAGCA 1441 GTGGGAATAG GAGCTTTGTT CCTTGGGTTC TTGGGAGCAG CAGGAAGCAC TATGGGCGCA 1501 GCGTCAATGA CGCTGACGGT ACAGGCCAGA CAATTATTGT CTGGTATAGT GCAGCAGCAG 1561 AACAATTTGC TGAGGGCTAT TGAGGCGCAA CAGCATCTGT TGCAACTCAC AGTCTGGGGC 1621 ATCAAGCAGC TCCAGGCAAG AATCCTGGCT GTGGAAAGAT ACCTAAAGGA TCAACAGCTC 1681 CTGGGGATTT GGGGTTGCTC TGGAAAACTC ATTTGCACCA CTGCTGTGCC TTGGAATGCT 1741 AGTTGGAGTA ATAAATCTCT GGAACAGATT TGGAATCACA CGACCTGGAT GGAGTGGGAC 1801 AGAGAAATTA ACAATTACAC AAGCTTAATA CACTCCTTAA TTGAAGAATC GCAAAACCAG 1861 CAAGAAAAGA ATGAACAAGA ATTATTGGAA TTAGATAAAT GGGCAAGTTT GTGGAATTGG 1921 TTTAACATAA CAAATTGGCT GTGGTATATA AAATTATTCA TAATGATAGT AGGAGGCTTG 1981 GTAGGTTTAA GAATAGTTTT TGCTGTACTT TCTATAGTGA ATAGAGTTAG GCAGGGATAT 2041 TCACCATTAT CGTTTCAGAC CCACCTCCCA ACCCCGAGGG GACCCGACAG GCCCGAAGGA 2101 ATAGAAGAAG AAGGTGGAGA GAGAGACAGA GACAGATCCA TTCGATTAGT GAACGGATCG 2161 GCACTGCGTG CGCCAATTCT GCAGACAAAT GGCAGTATTC ATCCACAATT TTAAAAGAAA 2221 AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG CAACAGACAT 2281 ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG TTTATTACAG 2341 GGACAGCAGA GATCCAGTTT GGTTAATTAG CTAGCTAGGT CTTGAAAGGA GTGGGAATTG 2401 GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG 2461 GAGGGGTCGG CAATTGATCC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG 2521 ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG 2581 TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GACCGGTTCT 2641 AGAGCCACCA TGAAACGGAC AGCCGACGGA AGCGAGTTCG AGTCACCAAA GAAGAAGCGG 2701 AAAGTCGACA AGAAGTACAG CATCGGCCTG GACATCGGCA CCAACTCTGT GGGCTGGGCC 2761 GTGATCACCG ACGAGTACAA GGTGCCCAGC AAGAAATTCA AGGTGCTGGG CAACACCGAC 2821 CGGCACAGCA TCAAGAAGAA CCTGATCGGA GCCCTGCTGT TCGACAGCGG CGAAACAGCC 2881 GAGGCCACCC GGCTGAAGAG AACCGCCAGA AGAAGATACA CCAGACGGAA GAACCGGATC 2941 TGCTATCTGC AAGAGATCTT CAGCAACGAG ATGGCCAAGG TGGACGACAG CTTCTTCCAC 3001 AGACTGGAAG AGTCCTTCCT GGTGGAAGAG GATAAGAAGC ACGAGCGGCA CCCCATCTTC 3061 GGCAACATCG TGGACGAGGT GGCCTACCAC GAGAAGTACC CCACCATCTA CCACCTGAGA 3121 AAGAAACTGG TGGACAGCAC CGACAAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC 3181 CACATGATCA AGTTCCGGGG CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC 3241 GACGTGGACA AGCTGTTCAT CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC 3301 CCCATCAACG CCAGCGGCGT GGACGCCAAG GCCATCCTGT CTGCCAGACT GAGCAAGAGC 3361 AGAAAGCTGG AAAATCTGAT CGCCCAGCTG CCCGGCGAGA AGAAGAATGG CCTGTTCGGA 3421 AACCTGATTG CCCTGAGCCT GGGCCTGACC CCCAACTTCA AGAGCAACTT CGACCTGGCC 3481 GAGGATGCCA AACTGCAGCT GAGCAAGGAC ACCTACGACG ACGACCTGGA CAACCTGCTG 3541 GCCCAGATCG GCGACCAGTA CGCCGACCTG TTTCTGGCCG CCAAGAACCT GTCCGACGCC 3601 ATCCTGCTGA GCGACATCCT GAGAGTGAAC ACCGAGATCA CCAAGGCCCC CCTGAGCGCC 3661 TCTATGATCA AGAGATACGA CGAGCACCAC CAGGACCTGA CCCTGCTGAA AGCTCTCGTG 3721 CGGCAGCAGC TGCCTGAGAA GTACAAAGAG ATTTTCTTCG ACCAGAGCAA GAACGGCTAC 3781 GCCGGCTACA TTGACGGCGG AGCCAGCCAG GAAGAGTTCT ACAAGTTCAT CAAGCCCATC 3841 CTGGAAAAGA TGGACGGCAC CGAGGAACTG CTCGTGAAGC TGAAGAGAGA GGACCTGCTG 3901 CGGAAGCAGC GGACCTTCGA CAACGGCAGC ATCCCCCACC AGATCCACCT GGGAGAGCTG 3961 CACGCCATTC TGCGGCGGCA GGAAGATTTT TACCCATTCC TGAAGGACAA CCGGGAAAAG 4021 ATCGAGAAGA TCCTGACCTT CCGCATCCCC TACTACGTGG GCCCTCTGGC CAGGGGAAAC 4081 AGCAGATTCG CCTGGATGAC CAGAAAGAGC GAGGAAACCA TCACCCCCTG GAACTTCGAG 4141 GAAGTGGTGG ACAAGGGCGC TTCCGCCCAG AGCTTCATCG AGCGGATGAC CAACTTCGAT 4201 AAGAACCTGC CCAACGAGAA GGTGCTGCCC AAGCACAGCC TGCTGTACGA GTACTTCACC 4261 GTGTATAACG AGCTGACCAA AGTGAAATAC GTGACCGAGG GAATGAGAAA GCCCGCCTTC 4321 CTGAGCGGCG AGCAGAAAAA GGCCATCGTG GACCTGCTGT TCAAGACCAA CCGGAAAGTG 4381 ACCGTGAAGC AGCTGAAAGA GGACTACTTC AAGAAAATCG AGTGCTTCGA CTCCGTGGAA 4441 ATCTCCGGCG TGGAAGATCG GTTCAACGCC TCCCTGGGCA CATACCACGA TCTGCTGAAA 4501 ATTATCAAGG ACAAGGACTT CCTGGACAAT GAGGAAAACG AGGACATTCT GGAAGATATC 4561 GTGCTGACCC TGACACTGTT TGAGGACAGA GAGATGATCG AGGAACGGCT GAAAACCTAT 4621 GCCCACCTGT TCGACGACAA AGTGATGAAG CAGCTGAAGC GGCGGAGATA CACCGGCTGG 4681 GGCAGGCTGA GCCGGAAGCT GATCAACGGC ATCCGGGACA AGCAGTCCGG CAAGACAATC 4741 CTGGATTTCC TGAAGTCCGA CGGCTTCGCC AACAGAAACT TCATGCAGCT GATCCACGAC 4801 GACAGCCTGA CCTTTAAAGA GGACATCCAG AAAGCCCAGG TGTCCGGCCA GGGCGATAGC 4861 CTGCACGAGC ACATTGCCAA TCTGGCCGGC AGCCCCGCCA TTAAGAAGGG CATCCTGCAG 4921 ACAGTGAAGG TGGTGGACGA GCTCGTGAAA GTGATGGGCC GGCACAAGCC CGAGAACATC 4981 GTGATCGAAA TGGCCAGAGA GAACCAGACC ACCCAGAAGG GACAGAAGAA CAGCCGCGAG 5041 AGAATGAAGC GGATCGAAGA GGGCATCAAA GAGCTGGGCA GCCAGATCCT GAAAGAACAC 5101 CCCGTGGAAA ACACCCAGCT GCAGAACGAG AAGCTGTACC TGTACTACCT GCAGAATGGG 5161 CGGGATATGT ACGTGGACCA GGAACTGGAC ATCAACCGGC TGTCCGACTA CGATGTGGAC 5221 GCTATCGTGC CTCAGAGCTT TCTGAAGGAC GACTCCATCG ACAACAAGGT GCTGACCAGA 5281 AGCGACAAGA ACCGGGGCAA GAGCGACAAC GTGCCCTCCG AAGAGGTCGT GAAGAAGATG 5341 AAGAACTACT GGCGGCAGCT GCTGAACGCC AAGCTGATTA CCCAGAGAAA GTTCGACAAT 5401 CTGACCAAGG CCGAGAGAGG CGGCCTGAGC GAACTGGATA AGGCCGGCTT CATCAAGAGA 5461 CAGCTGGTGG AAACCCGGCA GATCACAAAG CACGTGGCAC AGATCCTGGA CTCCCGGATG 5521 AACACTAAGT ACGACGAGAA TGACAAGCTG ATCCGGGAAG TGAAAGTGAT CACCCTGAAG 5581 TCCAAGCTGG TGTCCGATTT CCGGAAGGAT TTCCAGTTTT ACAAAGTGCG CGAGATCAAC 5641 AACTACCACC ACGCCCACGA CGCCTACCTG AACGCCGTCG TGGGAACCGC CCTGATCAAA 5701 AAGTACCCTA AGCTGGAAAG CGAGTTCGTG TACGGCGACT ACAAGGTGTA CGACGTGCGG 5761 AAGATGATCG CCAAGAGCGA GCAGGAAATC GGCAAGGCTA CCGCCAAGTA CTTCTTCTAC 5821 AGCAACATCA TGAACTTTTT CAAGACCGAG ATTACCCTGG CCAACGGCGA GATCCGGAAG 5881 CGGCCTCTGA TCGAGACAAA CGGCGAAACC GGGGAGATCG TGTGGGATAA GGGCCGGGAT 5941 TTTGCCACCG TGCGGAAAGT GCTGAGCATG CCCCAAGTGA ATATCGTGAA AAAGACCGAG 6001 GTGCAGACAG GCGGCTTCAG CAAAGAGTCT ATCCTGCCCA AGAGGAACAG CGATAAGCTG 6061 ATCGCCAGAA AGAAGGACTG GGACCCTAAG AAGTACGGCG GCTTCGACAG CCCCACCGTG 6121 GCCTATTCTG TGCTGGTGGT GGCCAAAGTG GAAAAGGGCA AGTCCAAGAA ACTGAAGAGT 6181 GTGAAAGAGC TGCTGGGGAT CACCATCATG GAAAGAAGCA GCTTCGAGAA GAATCCCATC 6241 GACTTTCTGG AAGCCAAGGG CTACAAAGAA GTGAAAAAGG ACCTGATCAT CAAGCTGCCT 6301 AAGTACTCCC TGTTCGAGCT GGAAAACGGC CGGAAGAGAA TGCTGGCCTC TGCCGGCGAA 6361 CTGCAGAAGG GAAACGAACT GGCCCTGCCC TCCAAATATG TGAACTTCCT GTACCTGGCC 6421 AGCCACTATG AGAAGCTGAA GGGCTCCCCC GAGGATAATG AGCAGAAACA GCTGTTTGTG 6481 GAACAGCACA AGCACTACCT GGACGAGATC ATCGAGCAGA TCAGCGAGTT CTCCAAGAGA 6541 GTGATCCTGG CCGACGCTAA TCTGGACAAA GTGCTGTCCG CCTACAACAA GCACCGGGAT 6601 AAGCCCATCA GAGAGCAGGC CGAGAATATC ATCCACCTGT TTACCCTGAC CAATCTGGGA 6661 GCCCCTGCCG CCTTCAAGTA CTTTGACACC ACCATCGACC GGAAGAGGTA CACCAGCACC 6721 AAAGAGGTGC TGGACGCCAC CCTGATCCAC CAGAGCATCA CCGGCCTGTA CGAGACACGG 6781 ATCGACCTGT CTCAGCTGGG AGGTGACtcc ggcggaagct ctggtggcag caagcggacc 6841 gccgacggct ctgaattcga gagccctaag aagaaaagaa aggtgagcgg aggctctagc 6901 ggcggaagcA CCCTGAACAT TGAAGACGAG TATAGACTGC ATGAAACAAG CAAGGAACCC 6961 GATGTGTCCC TGGGCTCCAC CTGGCTGTCC GACTTTCCCC AGGCCTGGGC CGAGACAGGA 7021 GGAATGGGCC TGGCCGTGCG GCAGGCACCC CTGATCATCC CTCTGAAGGC CACCTCTACA 7081 CCCGTGAGCA TCAAGCAGTA CCCTATGTCT CAGGAGGCCA GACTGGGCAT CAAGCCTCAC 7141 ATCCAGAGGC TGCTGGACCA GGGCATCCTG GTGCCATGCC AGAGCCCCTG GAACACACCA 7201 CTGCTGCCCG TGAAGAAGCC AGGCACCAAT GACTATAGAC CCGTGCAGGA TCTGAGAGAG 7261 GTGAACAAGA GGGTGGAGGA TATCCACCCC ACCGTGCCCA ACCCTTACAA TCTGCTGTCC 7321 GGCCTGCCCC CTTCTCACCA GTGGTATACA GTGCTGGACC TGAAGGATGC CTTCTTTTGT 7381 CTGAGACTGC ACCCTACCAG CCAGCCACTG TTCGCCTTTG AGTGGAGGGA CCCTGAGATG 7441 GGCATCTCTG GCCAGCTGAC CTGGACACGC CTGCCTCAGG GCTTCAAGAA TAGCCCAACA 7501 CTGTTTAACG AGGCCCTGCA CCGCGACCTG GCAGATTTCC GGATCCAGCA CCCAGATCTG 7561 ATCCTGCTGC AGTACGTGGA CGATCTGCTG CTGGCCGCCA CCAGCGAGCT GGATTGCCAG 7621 CAGGGAACAC GCGCCCTGCT GCAGACCCTG GGAAACCTGG GATATAGGGC ATCCGCCAAG 7681 AAGGCCCAGA TCTGTCAGAA GCAGGTGAAG TACCTGGGCT ATCTGCTGAA GGAGGGCCAG 7741 AGATGGCTGA CAGAGGCCAG GAAGGAGACA GTGATGGGCC AGCCAACACC CAAGACCCCA 7801 AGACAGCTGA GGGAGTTCCT GGGCAAAGCA GGATTTTGCA GGCTGTTCAT CCCAGGATTC 7861 GCAGAGATGG CAGCACCTCT GTACCCACTG ACCAAGCCGG GCACCCTGTT TAATTGGGGC 7921 CCTGACCAGC AGAAGGCCTA TCAGGAGATC AAGCAGGCCC TGCTGACAGC ACCAGCCCTG 7981 GGCCTGCCAG ACCTGACCAA GCCTTTCGAG CTGTTTGTGG ATGAGAAGCA GGGCTACGCC 8041 AAGGGCGTGC TGACCCAGAA GCTGGGACCA TGGAGACGGC CCGTGGCCTA TCTGTCCAAG 8101 AAGCTGGACC CAGTGGCAGC AGGATGGCCA CCATGCCTGA GGATGGTGGC AGCAATCGCC 8161 GTGCTGACAA AGGATGCCGG CAAGCTGACC ATGGGACAGC CACTGGTCAT CCTGGCACCA 8221 CACGCAGTGG AGGCCCTGGT GAAGCAGCCT CCAGATCGCT GGCTGTCTAA CGCCCGGATG 8281 ACACACTACC AGGCCCTGCT GCTGGACACC GATCGCGTGC AGTTTGGCCC TGTGGTGGCC 8341 CTGAATCCAG CCACCCTGCT GCCTCTGCCA GAGGAGGGCC TGCAGCACAA CTGTCTGGAC 8401 ATCCTGGCAG AGGCACACGG AACAAGGCCA GACCTGACCG ATCAGCCCCT GCCTGACGCC 8461 GATCACACAT GGTATACCGA TGGAAGCTCC CTGCTGCAGG AGGGCCAGAG GAAGGCAGGA 8521 GCAGCAGTGA CCACAGAGAC AGAAGTGATC TGGGCCAAGG CCCTGCCAGC AGGCACATCC 8581 GCCCAGCGGG CCGAGCTGAT CGCCCTGACC CAGGCCCTGA AGATGGCCGA GGGCAAGAAG 8641 CTGAACGTGT ACACAGACTC CAGATATGCC TTCGCCACCG CACACATCCA CGGAGAGATC 8701 TACAGGCGCC GGGGCTGGCT GACCTCTGAG GGCAAGGAGA TCAAGAACAA GGATGAGATC 8761 CTGGCCCTGC TGAAGGCCCT GTTTCTGCCC AAGCGGCTGA GCATCATCCA CTGTCCTGGA 8821 CACCAGAAGG GACACTCCGC CGAGGCAAGG GGCAATCGGA TGGCCGACCA GGCCGCCAGA 8881 AAGGCTGCTA TTACTGAAAC TCCCGACACT TCCACTCTGC TGATTGAAAA CTCCTCCCCT 8941 TCCGGAGGAT CTAGCGGAGG CTCCTCTGGC TCTGAGACAC CTGGCACAAG CGAGAGCGCA 9001 ACACCTGAAA GCAGCGGGGG CAGCAGCGGG GGGTCAATGG CTGAAAATGG TGATAATGAA 9061 AAGATGGCTG CCCTGGAGGC CAAAATCTGT CATCAAATTG AGTATTATTT TGGCGACTTC 9121 AATTTGCCAC GGGACAAGTT TCTAAAGGAA CAGATAAAAC TGGATGAAGG CTGGGTACCT 9181 TTGGAGATAA TGATAAAATT CAACAGGTTG AACCGTCTAA CAACAGACTT TAATGTAATT 9241 GTGGAAGCAT TGAGCAAATC CAAGGCAGAA CTCATGGAAA TCAGTGAAGA TAAAACTAAA 9301 ATCAGAAGGT CTCCAAGCAA ACCCCTACCT GAAGTGACTG ATGAGTATAA AAATGATGTA 9361 AAAAACAGAT CTGTTTATAT TAAAGGCTTC CCAACTGATG CAACTCTTGA TGACATAAAA 9421 GAATGGTTAG AAGATAAAGG TCAAGTACTA AATATTCAGA TGAGAAGAAC ATTGCATAAA 9481 GCATTTAAGG GATCAATTTT TGTTGTGTTT GATAGCATTG AATCTGCTAA GAAATTTGTA 9541 GAGACCCCTG GCCAGAAGTA CAAAGAAACA GACCTGCTAA TACTTTTCAA GGACGATTAC 9601 TTTGCCAAAA AAAATGAATC TGGCGGCTCA AAAAGAACCG CCGACGGCAG CGAATTCGAG 9661 TCTCCCAAGA AGAAGAGGAA AGTCGGCTCT GGCCCTGCCG CTAAGAGAGT GAAGCTGGAC 9721 GGATCCGGCG CAACAAACTT CTCTCTGCTG AAACAAGCCG GAGATGTCGA AGAGAATCCT 9781 GGACCGATGG CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT 9841 ACAATCAACA GCATCCCCAT CTCTGAAGAC TACAGCGTCG CCAGCGCAGC TCTCTCTAGC 9901 GACGGCCGCA TCTTCACTGG TGTCAATGTA TATCATTTTA CTGGGGGACC TTGTGCAGAA 9961 CTCGTGGTGC TGGGCACTGC TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG 10021 ATCGGAAATG AGAACAGGGG CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC 10081 GATCTGCATC CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT 10141 GGGATTCGTG AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAGCCGT GACCTACATG 10201 CTCGAAGGGC GTATGCGCCA CGAATTCGAT ATCAAGCTTA TCGGTAATCA ACCTCTGGAT 10261 TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 10321 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 10381 TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG 10441 CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC 10501 ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA 10561 CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT 10621 TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC 10681 TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT 10741 CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG 10801 ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGACC TCGAGACCTA 10861 GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG 10921 CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA 10981 CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG 11041 GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA 11101 CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG 11161 ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT 11221 GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG 11281 AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG 11341 CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT 11401 GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA 11461 GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA 11521 GTGTGGAAAA TCTCTAGCAG GGCCCGTTTA AACCCGCTGA TCAGCCTCGA CTGTGCCTTC 11581 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC 11641 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG 11701 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA 11761 TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG 11821 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 11881 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 11941 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT 12001 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 12061 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 12121 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 12181 GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA 12241 GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT 12301 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 12361 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 12421 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 12481 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG 12541 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 12601 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA TCATCGGCAT 12661 AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC AAGTTGACCA 12721 GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC TGGACCGACC 12781 GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC CGGGACGACG 12841 TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC CTGGCCTGGG 12901 TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG TCCACGAACT 12961 TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG GGGCGGGAGT 13021 TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG CAGGACTGAC 13081 ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 13141 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 13201 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 13261 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 13321 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 13381 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 13441 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 13501 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 13561 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 13621 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 13681 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 13741 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 13801 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 13861 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 13921 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 13981 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 14041 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 14101 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 14161 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 14221 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 14281 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 14341 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 14401 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 14461 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 14521 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 14581 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 14641 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 14701 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 14761 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 14821 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 14881 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 14941 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 15001 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 15061 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 15121 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 15181 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 15241 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 15301 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 15361 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 15421 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 15481 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG 15541 GATCGGGAGA TCTCCCGATC CCCTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC 15601 ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG 15661 CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG 15721 GGTTAGGCGT TTTGCGCTGC TTCGCGATGT ACGGGCCAGA TATA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.