pT7-ACLY-TEV-GST-6×His plasmid

pT7-ACLY-TEV-GST-6×His plasmid

PVT99874

pT7-ACLY-TEV-GST-6×His plasmid Information

Plasmid name: pT7-ACLY(human)-TEV-GST-6×His
Alias: NM_001096.3;ACL; ATPCL; CLATP
Gene lenght: 3303bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-ACLY-TEV-GST-6×His plasmid Description

pT7-ACLY-TEV-GST-6×His is a plasmid suitable for protein expression in Escherichia coli.

pT7-ACLY-TEV-GST-6×His plasmid Sequence

1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 5041 ttttgtttaa ctttaagaag gagatatacc atgtcggcca aggcaatttc agagcagacg 5101 ggcaaagaac tcctttacaa gttcatctgt accacctcag ccatccagaa tcggttcaag 5161 tatgctcggg tcactcctga cacagactgg gcccgcttgc tgcaggacca cccctggctg 5221 ctcagccaga acttggtagt caagccagac cagctgatca aacgtcgtgg aaaacttggt 5281 ctcgttgggg tcaacctcac tctggatggg gtcaagtcct ggctgaagcc acggctggga 5341 caggaagcca cagttggcaa ggccacaggc ttcctcaaga actttctgat cgagcccttc 5401 gtcccccaca gtcaggctga ggagttctat gtctgcatct atgccacccg agaaggggac 5461 tacgtcctgt tccaccacga ggggggtgtg gacgtgggtg atgtggacgc caaggcccag 5521 aagctgcttg ttggcgtgga tgagaaactg aatcctgagg acatcaaaaa acacctgttg 5581 gtccacgccc ctgaagacaa gaaagaaatt ctggccagtt ttatctccgg cctcttcaat 5641 ttctacgagg acttgtactt cacctacctc gagatcaatc cccttgtagt gaccaaagat 5701 ggagtctatg tccttgactt ggcggccaag gtggacgcca ctgccgacta catctgcaaa 5761 gtgaagtggg gtgacatcga gttccctccc cccttcgggc gggaggcata tccagaggaa 5821 gcctacattg cagacctcga tgccaaaagt ggggcaagcc tgaagctgac cttgctgaac 5881 cccaaaggga ggatctggac catggtggcc gggggtggcg cctctgtcgt gtacagcgat 5941 accatctgtg atctaggggg tgtcaacgag ctggcaaact atggggagta ctcaggcgcc 6001 cccagcgagc agcagaccta tgactatgcc aagactatcc tctccctcat gacccgagag 6061 aagcacccag atggcaagat cctcatcatt ggaggcagca tcgcaaactt caccaacgtg 6121 gctgccacgt tcaagggcat cgtgagagca attcgagatt accagggccc cctgaaggag 6181 cacgaagtca caatctttgt ccgaagaggt ggccccaact atcaggaggg cttacgggtg 6241 atgggagaag tcgggaagac cactgggatc cccatccatg tctttggcac agagactcac 6301 atgacggcca ttgtgggcat ggccctgggc caccggccca tccccaacca gccacccaca 6361 gcggcccaca ctgcaaactt cctcctcaac gccagcggga gcacatcgac gccagccccc 6421 agcaggacag catctttttc tgagtccagg gccgatgagg tggcgcctgc aaagaaggcc 6481 aagcctgcca tgccacaaga ttcagtccca agtccaagat ccctgcaagg aaagagcacc 6541 accctcttca gccgccacac caaggccatt gtgtggggca tgcagacccg ggccgtgcaa 6601 ggcatgctgg actttgacta tgtctgctcc cgagacgagc cctcagtggc tgccatggtc 6661 taccctttca ctggggacca caagcagaag ttttactggg ggcacaaaga gatcctgatc 6721 cctgtcttca agaacatggc tgatgccatg aggaagcatc cggaggtaga tgtgctcatc 6781 aactttgcct ctctccgctc tgcctatgac agcaccatgg agaccatgaa ctatgcccag 6841 atccggacca tcgccatcat agctgaaggc atccctgagg ccctcacgag aaagctgatc 6901 aagaaggcgg accagaaggg agtgaccatc atcggacctg ccactgttgg aggcatcaag 6961 cctgggtgct ttaagattgg caacacaggt gggatgctgg acaacatcct ggcctccaaa 7021 ctgtaccgcc caggcagcgt ggcctatgtc tcacgttccg gaggcatgtc caacgagctc 7081 aacaatatca tctctcggac cacggatggc gtctatgagg gcgtggccat tggtggggac 7141 aggtacccgg gctccacatt catggatcat gtgttacgct atcaggacac tccaggagtc 7201 aaaatgattg tggttcttgg agagattggg ggcactgagg aatataagat ttgccggggc 7261 atcaaggagg gccgcctcac taagcccatc gtctgctggt gcatcgggac gtgtgccacc 7321 atgttctcct ctgaggtcca gtttggccat gctggagctt gtgccaacca ggcttctgaa 7381 actgcagtag ccaagaacca ggctttgaag gaagcaggag tgtttgtgcc ccggagcttt 7441 gatgagcttg gagagatcat ccagtctgta tacgaagatc tcgtggccaa tggagtcatt 7501 gtacctgccc aggaggtgcc gcccccaacc gtgcccatgg actactcctg ggccagggag 7561 cttggtttga tccgcaaacc tgcctcgttc atgaccagca tctgcgatga gcgaggacag 7621 gagctcatct acgcgggcat gcccatcact gaggtcttca aggaagagat gggcattggc 7681 ggggtcctcg gcctcctctg gttccagaaa aggttgccta agtactcttg ccagttcatt 7741 gagatgtgtc tgatggtgac agctgatcac gggccagccg tctctggagc ccacaacacc 7801 atcatttgtg cgcgagctgg gaaagacctg gtctccagcc tcacctcggg gctgctcacc 7861 atcggggatc ggtttggggg tgccttggat gcagcagcca agatgttcag taaagccttt 7921 gacagtggca ttatccccat ggagtttgtg aacaagatga agaaggaagg gaagctgatc 7981 atgggcattg gtcaccgagt gaagtcgata aacaacccag acatgcgagt gcagatcctc 8041 aaagattacg tcaggcagca cttccctgcc actcctctgc tcgattatgc actggaagta 8101 gagaagatta ccacctcgaa gaagccaaat cttatcctga atgtagatgg tctcatcgga 8161 gtcgcatttg tagacatgct tagaaactgt gggtccttta ctcgggagga agctgatgaa 8221 tatattgaca ttggagccct caatggcatc tttgtgctgg gaaggagtat ggggttcatt 8281 ggacactatc ttgatcagaa gaggctgaag caggggctgt atcgtcatcc gtgggatgat 8341 atttcatatg ttcttccgga acacatgagc atgGAGAACC TATACTTCCA GGGTtcaatg 8401 tcccctatac taggttattg gaaaattaag ggccttgtgc aacccactcg acttcttttg 8461 gaatatcttg aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg 8521 cgaaacaaaa agtttgaatt gggtttggag tttcccaatc ttccttatta tattgatggt 8581 gatgttaaat taacacagtc tatggccatc atacgttata tagctgacaa gcacaacatg 8641 ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc ggttttggat 8701 attagatacg gtgtttcgag aattgcatat agtaaagact ttgaaactct caaagttgat 8761 tttcttagca agctacctga aatgctgaaa atgttcgaag atcgtttatg tcataaaaca 8821 tatttaaatg gtgatcatgt aacccatcct gacttcatgt tgtatgacgc tcttgatgtt 8881 gttttataca tggacccaat gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa 8941 cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta tatagcatgg 9001 cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa actcgagcac 9061 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct 9121 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg 9181 ggttttttgc tgaaaggagg aactatatcc ggat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.