pLV3-CMV-ADISSP-3×FLAG-CopGFP-

pLV3-CMV-ADISSP-3×FLAG-CopGFP-Puro plasmid

PVT99369

pLV3-CMV-ADISSP-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ADISSP(human)-3×FLAG-CopGFP-Puro
Alias: NM_001039140.3;C20orf27
Gene lenght: 597bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ADISSP-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ADISSP-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ADISSP-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ctgcagccaa caagggtaag 2341 tgccttcctg gcgtggtagg acttgcacaa gctcttccgg tgggccctgg taggagggcc 2401 attgctgcag gcaacaagcc cagagtccgg agtatccgct ttgcggcagg ccacgatgca 2461 gaaggatccc acagccacgt ccactttgat gagaagctgc atgactcggt ggtcatggtc 2521 acccaggaga gtgacagcag ctttctggtc aaggttggct tcctgaagat cctgcacagg 2581 tatgagatta ccttcactct gcccccagtg cacaggctga gcaaggatgt ccgcgaggca 2641 cctgtcccca gcctgcacct caagctcctc agcgtggtgc ccgtccctga aggttatagt 2701 gtcaagtgtg agtactcggc gcacaaagag ggcgtcctca aagaggagat actgctagcc 2761 tgcgaaggtg gcactggcac ctgtgtgcgc gtgacggtgc aggcccgcgt catggaccgg 2821 caccacggca cgcccatgct gctggatggt gtcaagtgtg tgggcgccga gctggaatac 2881 gactcagagc acagcgactg gcacggcttt gacggatccG ACTACAAAGA CCATGACGGT 2941 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 3001 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 3061 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 3121 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 3181 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 3241 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 3301 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 3361 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 3421 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 3481 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 3541 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 3601 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 3661 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 3721 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 3781 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 3841 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 3901 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 3961 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 4021 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 4081 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 4141 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 4201 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 4261 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 4321 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 4381 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 4441 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 4501 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 4561 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 4621 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 4681 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 4741 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 4801 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 4861 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 4921 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 4981 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 5041 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 5101 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 5161 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 5221 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 5281 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 5341 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 5401 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 5461 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 5521 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 5581 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 5641 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 5701 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 5761 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 5821 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 5881 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 5941 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6001 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 6061 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 6121 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 6181 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 6241 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 6301 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 6361 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 6421 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 6481 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 6541 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 6601 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 6661 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 6721 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 6781 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 6841 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 6901 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 6961 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 7021 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 7081 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 7141 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 7201 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 7261 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 7321 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 7381 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 7441 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 7501 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 7561 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7621 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 7681 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 7741 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 7801 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 7861 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7921 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7981 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 8041 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 8101 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 8161 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 8221 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 8281 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 8341 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 8401 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 8461 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 8521 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 8581 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 8641 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 8701 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 8761 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 8821 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 8881 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.