pLV3-CMV-RBP2-3×FLAG-CopGFP-Pu

pLV3-CMV-RBP2-3×FLAG-CopGFP-Puro plasmid

PVT99109

pLV3-CMV-RBP2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-RBP2(human)-3×FLAG-CopGFP-Puro
Alias: NM_004164.3;CRABP-II; CRBP2; CRBPII; RBPC2
Gene lenght: 402bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-RBP2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-RBP2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-RBP2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga caagggacca gaatggaacc 2341 tgggagatgg agagtaatga aaactttgag ggctacatga aggccctgga tattgatttt 2401 gccacccgca agattgcagt acgtctcact cagacgaagg ttattgatca agatggtgat 2461 aacttcaaga caaaaaccac tagcacattc cgcaactatg atgtggattt cactgttgga 2521 gtagagtttg acgagtacac aaagagcctg gataaccggc atgttaaggc actggtcacc 2581 tgggaaggtg atgtccttgt gtgtgtgcaa aagggggaga aggagaaccg cggctggaag 2641 cagtggattg agggggacaa gctgtacctg gagctgacct gtggtgacca ggtgtgccgt 2701 caagtgttca aaaagaaagg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 2761 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 2821 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 2881 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 2941 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 3001 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3061 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 3121 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 3181 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 3241 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 3301 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 3361 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 3421 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 3481 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 3541 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 3601 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 3661 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 3721 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 3781 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 3841 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 3901 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 3961 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 4021 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4081 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 4141 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 4201 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 4261 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 4321 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 4381 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 4441 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 4501 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 4561 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 4621 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 4681 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 4741 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 4801 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 4861 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 4921 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 4981 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5041 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5101 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 5161 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 5221 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 5281 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 5341 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 5401 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 5461 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 5521 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 5581 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 5641 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 5701 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 5761 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 5821 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 5881 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 5941 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 6001 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 6061 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 6841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 6901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 6961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 7861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 7921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 7981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 8281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 8341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 8401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 8461 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8521 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 8581 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 8641 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.