pLV3-U6-BNC2-shRNA-EF1a-Puro p

pLV3-U6-BNC2-shRNA-EF1a-Puro plasmid

PVT98808

pLV3-U6-BNC2-shRNA-EF1a-Puro plasmid Information

Plasmid name: pLV3-U6-BNC2(rat)-shRNA-EF1a-Puro
Alias: GGGCAAGAGACTTGACTTT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-BNC2-shRNA-EF1a-Puro plasmid Description

pLV3-U6-BNC2-shRNA-EF1a-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-BNC2-shRNA-EF1a-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tCGGGTTTAT TACAGGGACA GCAGAGATCC AGTTTATCGA TCTGGGCAGG AAGAGGGCCT 1981 ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTAG 2041 AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA 2101 ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC 2161 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGATC 2221 CGGGGCAAGA GACTTGACTT TCTCGAGAAA GTCAAGTCTC TTGCCCTTTT TTGAATTCga 2281 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 2341 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 2401 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 2461 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 2521 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 2581 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 2641 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 2701 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 2761 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 2821 cctacgctag atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 2881 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 2941 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 3001 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 3061 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 3121 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 3181 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 3241 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 3301 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 3361 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 3421 cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 3481 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 3541 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 3601 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 3661 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 3721 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 3781 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc 3841 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 3901 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 3961 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 4021 cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 4081 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt 4141 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 4201 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 4261 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 4321 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 4381 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 4441 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 4501 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 4561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 4621 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 4681 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa 4741 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 4801 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 4861 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 4921 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 4981 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 5041 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 5101 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 5161 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 5221 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 5281 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 5341 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 5401 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 5461 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 5521 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 5581 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 5641 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 5701 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 5761 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 5821 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 5881 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 5941 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 6001 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 6061 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 6121 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 6181 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 6241 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 6301 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 6361 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 6421 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 6481 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6541 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6601 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6661 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6721 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 6781 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 6841 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 6901 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 6961 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 7021 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 7081 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 7141 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 7201 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 7261 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 7321 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.