pCMV-BRD4-2-CMV-EGFP-Neo plasm

pCMV-BRD4-2-CMV-EGFP-Neo plasmid

PVT98720

pCMV-BRD4-2-CMV-EGFP-Neo plasmid Information

Plasmid name: pCMV-BRD4(human)-2-CMV-EGFP-Neo
Alias: NM_014299.3;CAP; CDLS6; FSHRG4; HUNK1; HUNKI; MCAP
Gene lenght: 2169bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-BRD4-2-CMV-EGFP-Neo plasmid Description

pCMV-BRD4-2-CMV-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-BRD4-2-CMV-EGFP-Neo plasmid Sequence

1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 781 aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt 841 tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa 901 cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg 961 accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta 1021 ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc 1081 ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg 1141 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat 1201 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca 1261 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc 1321 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt 1381 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc 1441 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac 1501 gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag 1561 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg 1621 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt 1681 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga 1741 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt 1801 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc 1861 tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg 1921 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc 1981 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc 2041 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta 2101 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg 2161 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg 2221 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat 2281 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg 2341 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac 2401 accctaactg acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca 2461 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2521 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgctta 2581 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 2641 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 2701 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 2761 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga gtcgcggccg 2821 ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc 2881 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg 2941 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg 3001 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg 3061 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc 3121 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc 3181 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg 3241 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc 3301 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg 3361 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg 3421 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg 3481 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc 3541 atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg actgcaggga tctgacggtt 3601 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt 3661 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa 3721 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc 3781 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta 3841 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg 3901 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac 3961 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat 4021 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg 4081 tcagccaggc gggccattta ccgtaagtta tgtaacggtg atgcggtttt ggcagtacat 4141 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 4201 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc 4261 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 4321 tctctggcta actagagaac ccactgctta ctggcttatc ggctagcgct accggactca 4381 gatctcgagg ccaccatgtc tgcggagagc ggccctggga cgagattgag aaatctgcca 4441 gtaatggggg atggactaga aacttcccaa atgtctacaa cacaggccca ggcccaaccc 4501 cagccagcca acgcagccag caccaacccc ccgcccccag agacctccaa ccctaacaag 4561 cccaagaggc agaccaacca actgcaatac ctgctcagag tggtgctcaa gacactatgg 4621 aaacaccagt ttgcatggcc tttccagcag cctgtggatg ccgtcaagct gaacctccct 4681 gattactata agatcattaa aacgcctatg gatatgggaa caataaagaa gcgcttggaa 4741 aacaactatt actggaatgc tcaggaatgt atccaggact tcaacactat gtttacaaat 4801 tgttacatct acaacaagcc tggagatgac atagtcttaa tggcagaagc tctggaaaag 4861 ctcttcttgc aaaaaataaa tgagctaccc acagaagaaa ccgagatcat gatagtccag 4921 gcaaaaggaa gaggacgtgg gaggaaagaa acagggacag caaaacctgg cgtttccacg 4981 gtaccaaaca caactcaagc atcgactcct ccgcagaccc agacccctca gccgaatcct 5041 cctcctgtgc aggccacgcc tcaccccttc cctgccgtca ccccggacct catcgtccag 5101 acccctgtca tgacagtggt gcctccccag ccactgcaga cgcccccgcc agtgcccccc 5161 cagccacaac ccccacccgc tccagctccc cagcccgtac agagccaccc acccatcatc 5221 gcggccaccc cacagcctgt gaagacaaag aagggagtga agaggaaagc agacaccacc 5281 acccccacca ccattgaccc cattcacgag ccaccctcgc tgcccccgga gcccaagacc 5341 accaagctgg gccagcggcg ggagagcagc cggcctgtga aacctccaaa gaaggacgtg 5401 cccgactctc agcagcaccc agcaccagag aagagcagca aggtctcgga gcagctcaag 5461 tgctgcagcg gcatcctcaa ggagatgttt gccaagaagc acgccgccta cgcctggccc 5521 ttctacaagc ctgtggacgt ggaggcactg ggcctacacg actactgtga catcatcaag 5581 caccccatgg acatgagcac aatcaagtct aaactggagg cccgtgagta ccgtgatgct 5641 caggagtttg gtgctgacgt ccgattgatg ttctccaact gctataagta caaccctcct 5701 gaccatgagg tggtggccat ggcccgcaag ctccaggatg tgttcgaaat gcgctttgcc 5761 aagatgccgg acgagcctga ggagccagtg gtggccgtgt cctccccggc agtgccccct 5821 cccaccaagg ttgtggcccc gccctcatcc agcgacagca gcagcgatag ctcctcggac 5881 agtgacagtt cgactgatga ctctgaggag gagcgagccc agcggctggc tgagctccag 5941 gagcagctca aagccgtgca cgagcagctt gcagccctct ctcagcccca gcagaacaaa 6001 ccaaagaaaa aggagaaaga caagaaggaa aagaaaaaag aaaagcacaa aaggaaagag 6061 gaagtggaag agaataaaaa aagcaaagcc aaggaacctc ctcctaaaaa gacgaagaaa 6121 aataatagca gcaacagcaa tgtgagcaag aaggagccag cgcccatgaa gagcaagccc 6181 cctcccacgt atgagtcgga ggaagaggac aagtgcaagc ctatgtccta tgaggagaag 6241 cggcagctca gcttggacat caacaagctc cccggcgaga agctgggccg cgtggtgcac 6301 atcatccagt cacgggagcc ctccctgaag aattccaacc ccgacgagat tgaaatcgac 6361 tttgagaccc tgaagccgtc cacactgcgt gagctggagc gctatgtcac ctcctgtttg 6421 cggaagaaaa ggaaacctca agctgagaaa gttgatgtga ttgccggctc ctccaagatg 6481 aagggcttct cgtcctcaga gtcggagagc tccagtgagt ccagctcctc tgacagcgaa 6541 gactccgaaa caggtcctgc ctaagaattc tcatctagac tggaaaTggg ggaggctaac 6601 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 6661 gaataaaacg cacgggtgtt gggtcgtttg ttc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.