pCMV-Polq-3×FLAG-Neo plasmid

pCMV-Polq-3×FLAG-Neo plasmid

PVT98694

pCMV-Polq-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Polq(mouse)-3×FLAG-Neo
Alias: NM_029977.2;A430110D14Rik
Gene lenght: 7632bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl2
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Polq-3×FLAG-Neo plasmid Description

pCMV-Polq-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Polq-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgag tcttccgcgc cggagtagga 781 aacggcggcg ttcatcgtcc ggctccgaca cgttctcggg agatggtgat agctttgtta 841 gccctcagct ccggtgtgga ccggtgctga gtccacctcc ggggctggga cgcggccgga 901 ggctcacagg gacaggaaca aacaagcgaa gagtttctga tgaccagata gaccagctgc 961 tgttggcaaa ttggggactt cctaaagcag ttctggagaa ataccatagt tttggtgtaa 1021 gaaagatgtt cgaatggcag gcagaatgcc ttttgcttgg tcacgtcttg gaaggaaaga 1081 atctagttta ttcagctcct acaagtgctg ggaagactct tgttgcagag ttacttattc 1141 tgaagcgggt tttggaaaca cggaagaaag cgttgtttat tttgcccttt gtgtctgtgg 1201 ctaaagagaa gaaatgctac ctccagagtc tgtttcagga agtaggacta aaggtagatg 1261 gctatatggg cagcacctca ccaacgggac agttctcctc cttggacatt gctgtttgca 1321 ctattgagag agccaatggg ctggtcaatc gcctcattga ggaaaataag atggatctgt 1381 taggaatggt ggtcgtggat gagctgcaca tgctggggga ctctcaccga gggtatctgc 1441 tggagctgtt gctgaccaag atttgctacg tcactcgtaa atcagcatcg caccaggcag 1501 agtcagccag cactctgtct aatgctgtgc agattgttgg catgagtgct actcttccta 1561 atttgcagct tgtggcttcc tggctgaatg ctgaacttta ccataccgac tttcgccctg 1621 taccactttt ggaatcaata aaaataggaa attccatata tgattcttca atgaagcttg 1681 tgagagaatt tcaacccctg ctccaagtga agggagatga agaccacatt gttagtttat 1741 gctatgagac cattcaggat aaccattcag tattaatctt ttgtccatca aaaaaatggt 1801 gtgagaaggt agcagatatc attgcccgag agttttacaa tctgcaccat caacctgagg 1861 ggttggtaaa atcatctgaa tttccaccag tgattctaga ccaaaagagc cttctggaag 1921 tgatggatca gttaaaacgt tcaccttcag gactagactc tgtgttgaag aatactgtgc 1981 catggggagt agcgtttcat catgcaggtc ttacttttga ggagagggat atcatcgaag 2041 gagccttccg tcaaggtttc attcgggtct tggcagcaac gtctactctt tcttctggag 2101 tgaatcttcc tgctcgtcgg gtgattattc gcactccaat tttcagtggt cagcctttag 2161 atatcctcac ttataagcag atggttgggc gtgctggcag gaaaggagta gacacaatgg 2221 gtgaaagtat cctagtttgt aagaactctg agaaatccaa gggcattgct ctgcttcaag 2281 gatctctgga gcctgtgcac agctgtctgc agagacaggg agaagtcact gctagcatga 2341 tacgagctat tctggagata atcgttggtg gggtggccag tacgtcacaa gatatgcaga 2401 cttatgccgc ctgcaccttc ttggctgctg ctatacagga agggaagcaa gggatgcaga 2461 gaaatcaaga tgatgctcag cttggagcga ttgatgcctg tgtgacgtgg ctgttagaaa 2521 atgagttcat ccaggtggca gagcccgggg acggcacagg aggaaaggtg tatcacccaa 2581 cacatcttgg atcagctact ctttcttctt cactgtctcc aactgatacc cttgatattt 2641 ttgctgacct ccaaagagca atgaaaggct ttgttttaga gaatgacctt catattgtct 2701 acctggttac acctgtgttt gaagattgga ttagtattga ttggtaccga tttttctgtt 2761 tatgggaaaa attaccaact tctatgaaaa gggtggcaga gctggttgga gtggaggaag 2821 ggttcttggc tcgatgtgtg aaaggaaaag tagtagctag aactgagaga cagcatcggc 2881 agatggccat ccataaaagg tttttcacaa gtcttgtgtt actggattta atcagtgaaa 2941 ttcccttaaa ggacattaat cagaagtatg gatgtaatcg tggacagatt cagtctttgc 3001 aacagtcagc tgctgtctat gcaggaatga ttacggtgtt ttccaaccgc ctgggctggc 3061 acaacatgga actactgctt tcccagtttc agaagcgtct tacgtttggc atccagagag 3121 agctgtgtga cctcatccgg gtgtccttac tcaacgcgca gagagccagg ttcctgtatg 3181 cctctggctt tctcaccgtg gcagatcttg ccagagctga tagtgcggag gtagaggtgg 3241 ctctgaagaa ctctttgcct ttcaaaagtg cccggaaggc agtggatgaa gaagaggaag 3301 cagctgaaga acggcggagt atgagaacta tttgggtgac tggcaagggc ttatctgcaa 3361 gggaagcagc agccttaata gtggaagaag ctaaaatgat tctacagcag gacttaattg 3421 aaatgggagt acggtgggat ccgaagtcac ccttgagttc tagcacacac tcacggacca 3481 gcacgtcaga agtaaaggaa cacacgttca aatcccagac taagagttct cataaaaggt 3541 tagcatcaat gggcagaaat agcattagag caagtgggtc taatgacaag ccgtccccag 3601 atgcagagcg aggcatagat gactgcagcg agcatgcaga ctctctctgt aagttccagg 3661 gcaatttcga gccccagaca ccctccattt gtacagcaag aaaacggact tctttaggta 3721 taaacaaaga aatgcttcga aagtctctga aagaaggaaa gccaagcact aaggaagttc 3781 ttcaaacttt ctcatcggag aaaacaagaa aaactgcttt gagttttagt tcagagcaag 3841 tgaacaacac tttaccatct gggagagaca gaaagtatca gaaaaaatct tggggcagta 3901 gtcctgtgcg tgactctggg atgcatagag gtgatttaca gggacagact atgtgtacat 3961 ctgctctatg tgaagactca cagaagtcct tagaggagca gaatgcagaa tttagaagtc 4021 cagggctatt tgcaaaacat ctaccttctt gtgccaaaga aaaatgcaag aagccctcac 4081 tccctttaca aaggcagcag gcttgctcaa ggagaagcac agagagctgt gctgctgtgg 4141 ggcatcccgc tgcagggtcg tcccctgctg ctgctagaga cagaagagga ctggctgctc 4201 gggagacaga aaagggaaat gaggcgctca cggagaatgg tggagaaagc cagctccaag 4261 acacctaccc agtcagccag tacctggaat accacagtga gaaacacaca aacacttgca 4321 ccagacagaa gacattaaca gagggacaag ctggtagtag ttatgtggcc agagactcaa 4381 atgatgctgc acccatcaaa tgtgaacgta tgaagcttaa tagcaaagac cgcgactcaa 4441 atccttgtcg tcaggcattg ggaagttaca caggcaggac tgaggcactt cagtcaactg 4501 caaaacttgg ccaagcagga ggccagtgtg aaaatcttct aaattcttct ggagtacaag 4561 gaaaaacagg tgctcatgca acaaatagaa ctgaacatag tcatgcttct aacccagcct 4621 tctgtgactt tggagatagc ttagacttgg atactcagtc agaggaaata attgagcaga 4681 tggcaactga aaataccatg caaggagcaa aagcagtagt gataatggaa gagggttccg 4741 ccatgcagaa caagtgtcac agcactccag gtgaccagca tgtcccagga gcagccaaca 4801 cagaccatgt ggacagtaag aaggttgagt ctgtgaaagc aaacaccgaa aagaacatca 4861 atagaggcgc tccagtcagc ctcatcttcc atactcaagg ggaaaatggc gcttgcttta 4921 aagggaatga gcattctgtt actgattccc aattaaacag ttttcttcaa gggtttgaaa 4981 cacaagaaat agtgaagcca atcataccgc tggctcctca aatgagaact cctactggtg 5041 tggaggaaga aagtctgcct gaaaccagtt tgaatatgag tgatagtata ctatttgaca 5101 gttttggaga agatggcttc ggacaaggac agtcacctga tataaaggca aatcagccac 5161 tcctttctga aatgacacca aaccatttca gcaatcctcc acacccacaa gaagacccag 5221 ttatgacacc aactgtgagt gagcctcagg gtactcagca gcaaggagtt tgtctcagtg 5281 gtgaatctat tatattttca gacatagact ctgctcaggt cattgaagcc ttagacaata 5341 tggctgcatt tcatgtgcaa gagaactgta actcagtagc ccttaaaaca ttagaaccca 5401 gtgattctgc agtgctgggt aatgagtgtc cccaaggaaa actggtcaga ggagatcaaa 5461 atgaagggtc cccaaagccc aaactcacag agacaaatca agataattca ttcacttggt 5521 caggagcatc atttaactta agtccagaac tgcaaaggat tttagataaa gtatctagtc 5581 ctcgagaaaa tgaaaagccc aagatgatac atgtaaactt gtcttccttt gaaggaaaca 5641 gcaaagagtc acatgaaaga gaggaaataa attcagattt ggggacagtt cagagaactt 5701 cagttttccc cagtaatgaa gttaaaaaca ggactgaagg gctagagagc aaagccaggc 5761 atggtggagc ctcatctccc ttgcctcgta aagaaagtgc tgcagctgat gacaatggcc 5821 tcattcctcc tacacccgtt cctgcttctg cttctaaggt agcctttcca gagattctcg 5881 gaacatctgt aaaacgtcag aaagccagca gtgctttaca gccaggggaa agttgtttat 5941 ttggctcacc ttcagacaat caaaaccagg atttgagtca agagcttaga gacagcctca 6001 aggactatga tggttctgtt gcagacacaa gtttttttct gcagtctcag gatggactgc 6061 tgttaactca agcctcatgc agctcagaga gtctggccat aattgacgta gcgagtgatc 6121 agattctctt tcagacattt gtgaaggagt ggcaatgcca aaagcgcttt tccatctctc 6181 tggcttgtga aaagatgaca agttcaatgt cttccaaaac tgctaccata ggtggaaagt 6241 tgaagcaagt tagcttgcca caggaagcca ctgttgaaga tgctgggttc cctgtgcgtg 6301 gctgtgatgg tgctgtggta gttggactag cagtgtgctg gggtgcaaag gatgcctatt 6361 atttgtcact gcagaaggaa caaaagcagt ctgaaataag tcccagtttg gccccaccac 6421 ctctggacgc aacactaact gtgaaggaga ggatggagtg ccttcagtcc tgcttgcaaa 6481 agaagtctga tcgagagcgg tctgttgtca cctatgattt catccagacc tataaagtcc 6541 tcctcctgtc atgtggcatc tccctggagc ccagttatga agaccccaag gtggcatgct 6601 ggcttctcga cccagattct aaggagccaa ctcttcatag catagtgacc agttttctcc 6661 cccatgagct agcactccta gaaggaatgg agacaggccc aggaattcag agcctggggc 6721 taaatgtgaa cactgaacat tctgggaggt acagagcatc tgtggagtct gttctcatct 6781 tcaactccat gaatcaactc aactctttgt tgcagaagga aaaccttcat gatattttct 6841 gcaaagtgga gatgccctct cagtactgcc tggccttgct agaactaaat ggaattggct 6901 ttagtactgc ggagtgtgaa agccagaagc atgttatgca agccaagctg gacgccattg 6961 agacccaagc ctatcagctg gctggccaca gtttttcctt tacaagtgca gatgacatcg 7021 cacaggtttt atttttggaa ctaaagttac caccgaatgg agagatgaaa acgcaaggca 7081 gcaaaaagac tctgggttcc accagaagag ggaatgagag tggccgcaga atgaggctgg 7141 gaagacagtt cagcactagt aaggatattt taaataaatt aaagggcttg catcctttac 7201 caggcctgat actggaatgg agaagaatca gtaatgctat taccaaggtg gtcttccctc 7261 tgcagcggga aaagcacctg aacccccttc tcagaatgga gaggatctac ccggtgtcac 7321 agtcacacac ggctacagga cggataacct ttacagaacc caatatccag aatgtgccaa 7381 gagattttga gattaaaatg ccaacactag taagggagag cccaccttct caagctccaa 7441 aaggccggtt cccaatggca ataggacaag ataagaaggt ttatggcctg caccccgggc 7501 acagaacaca gatggaagag aaggcctcag atagaggggt gccattttca gtgagcatgc 7561 gccatgcctt tgtgcctttc ccaggtgggc tgatcctggc tgccgactac tctcagcttg 7621 aactgaggat cctggctcac ttgtctcgtg actgtcgtct cattcaagtc ttaaatacgg 7681 gagcggatgt gttcaggagc attgctgccg agtggaagat gattgagcct gatgctgttg 7741 gtgacgatct gaggcagcac gcaaagcaga tttgctatgg aattatatat ggaatgggag 7801 ctaaatcttt aggagagcag atgggcatta aagaaaatga tgctgcttcc tacattgact 7861 ctttcaagtc cagatataaa gggattaatc atttcatgag ggacacagtg aagaactgta 7921 gaaaaaatgg atttgttgag acaattttgg gaagacgtag atatttgcca ggaatcaaag 7981 acgacaatcc ttatcataaa gcacacgctg aacgtcaggc catcaacaca acagtccagg 8041 gatcagctgc tgatatagtc aaaatagcca cagttaatat tcagaagcaa ttagagacct 8101 tccgttcaac cttcaaatcc cacggtcatc gggaaagtat gctccagaat gaccgaacag 8161 gattgttgcc caagaggaaa cttaaaggga tgttttgtcc catgagagga ggattcttca 8221 tccttcaact tcacgacgag cttttatatg aagtggcaga agaggatgtt gttcaggtgg 8281 ctcagattgt caagaatgaa atggaatgtg ctataaaact ttctgtgaag ctgaaagtga 8341 aagtgaaaat aggtgccagc tggggagagc tgaaggactt tgatgtgGGA TCCGACTACA 8401 AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT 8461 AGTGAGGGCC CGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 8521 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 8581 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 8641 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 8701 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 8761 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 8821 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT 8881 GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 8941 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 9001 TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC 9061 CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA 9121 GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT 9181 TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC 9241 CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG 9301 TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG 9361 CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 9421 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 9481 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 9541 GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 9601 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 9661 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 9721 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT ATTGGTTAAA 9781 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA 9841 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 9901 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 9961 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 10021 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 10081 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 10141 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 10201 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 10261 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 10321 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 10381 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 10441 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 10501 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 10561 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 10621 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 10681 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 10741 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 10801 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 10861 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 10921 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 10981 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA 11041 AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT 11101 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG 11161 CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG 11221 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC 11281 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC 11341 TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT 11401 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG 11461 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT 11521 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG 11581 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC 11641 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 11701 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 11761 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 11821 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 11881 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 11941 GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 12001 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 12061 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 12121 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 12181 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 12241 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 12301 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 12361 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 12421 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 12481 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 12541 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 12601 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 12661 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 12721 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 12781 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 12841 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 12901 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 12961 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 13021 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 13081 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 13141 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 13201 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 13261 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 13321 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 13381 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 13441 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 13501 CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTCGACT 13561 CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG 13621 TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC 13681 GACAATTGCA TGAAGAATCT GCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.