pLV3-U6-LRP1-shRNA1-Puro plasm

pLV3-U6-LRP1-shRNA1-Puro plasmid

PVT97622

pLV3-U6-LRP1-shRNA1-Puro plasmid Information

Plasmid name: pLV3-U6-LRP1(human)-shRNA1-Puro
Alias: GCAAGAAGCCGGAGCATGA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-LRP1-shRNA1-Puro plasmid Description

pLV3-U6-LRP1-shRNA1-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-LRP1-shRNA1-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgaattc gGCAAGAAGC CGGAGCATGA ttcaagagaT 2221 CATGCTCCGG CTTCTTGCtt ttttggatcc gcggccgcGa aggatctgcg atcgctccgg 2281 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 2341 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 2401 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 2461 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 2521 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 2581 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 2641 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 2701 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 2761 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag acGCCACCat 2821 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 2881 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 2941 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 3001 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 3061 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 3121 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 3181 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 3241 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 3301 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 3361 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa 3421 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 3481 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 3541 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 3601 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 3661 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 3721 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 3781 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 3841 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 3901 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg 3961 ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag 4021 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 4081 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct 4141 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 4201 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 4261 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 4321 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 4381 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 4441 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 4501 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct 4561 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 4621 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc 4681 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 4741 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 4801 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 4861 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 4921 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 4981 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 5041 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5101 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 5161 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 5221 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 5281 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 5341 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 5401 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 5461 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 5521 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 5581 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 5641 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 5701 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 5761 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 5821 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 5881 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 5941 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 6001 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 6061 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 6121 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 6181 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 6241 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 6301 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 6361 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 6421 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 6481 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 6541 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 6601 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 6661 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 6721 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 6781 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 6841 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 6901 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 6961 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 7021 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 7081 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 7141 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 7201 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 7261 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag 7321 ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.