pLV3-CMV-Inpp5f-3×HA-CopGFP-Pu

pLV3-CMV-Inpp5f-3×HA-CopGFP-Puro plasmid

PVT97566

pLV3-CMV-Inpp5f-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Inpp5f(rat)-3×HA-CopGFP-Puro
Alias: NM_001107554.1
Gene lenght: 3390bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Inpp5f-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-Inpp5f-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Inpp5f-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg agctctttca ggccaaggac 2341 cactacatcc tgcagcaggg cgagcgtgcc ctgtggtgca gccggcgcga cggcggcctg 2401 cagctccgac cggccactga cctacttctt gcctggaacc ccatttgttt gggattggta 2461 gaaggagtaa ttgggaagat tcaactccat tcagatcttc cgtggtggct tattttaatt 2521 cggcagaaag cattggtggg caaactccca ggagaccatg aggtctgtaa agtcaccaag 2581 attgctgtat tgtcactttc tgaaatggaa cctcaggagc ttgagctgga gctctgcaag 2641 aagcatcatt ttggaattaa caagcctgag aagattgtac catctcctga tgactccaag 2701 tttctactga agacattcac aaatatcaaa tccaatgtgt ctgctcccaa caaaaagaaa 2761 gttaaagaaa gtaaagaaaa ggagaaactg gagaggcgtt tgcttgagga gttgctgaag 2821 atgttcatgg actcagagtc cttctattat agcttgacct atgacctaac caactcagtg 2881 cagaggcaga gcactgggga gaaggatggc cgccccctgt ggcagaaggt tgatgatcga 2941 tttttctgga ataagtacat gatccaagac ctctctgaga ttggtactcc ggacgtggac 3001 ttctggatca tccccattat ccagggtttt gtgcagattg aagaactggt ggttaattac 3061 gagccatctg atgatgacaa gagcagccca gagacgcccc ctcaggaggc cacttgtgta 3121 gatgacattc acccgcgatt tctagtggct cttatatctc gcaggagtag gcacagagca 3181 ggaatgcggt ataaacgaag aggagtggat aaaaatggaa atgttgcaaa ttatgtggag 3241 actgagcagt taattcatgt tcataatcat actctgtcgt ttattcaaac tcgaggctcc 3301 gtgcccgtct tttggagcca ggttgggtat cgctataatc caagacctcg actagacaaa 3361 agtgagaagg agacagtcga ctgtttctgt gcccattttg aagaacaact gaaaatttac 3421 aaaaagcagg ttattgttaa cttggtggac caggctggaa gggagaagat cattggtgat 3481 gcttacctga agcaggtgct gctcttcaac aaccccaggc tcacctacgt ctcctttgat 3541 ttccacgagc actgccgagg aatgaagttt gagaatgttc aaaccctaac ggatgccatt 3601 catgacatca tccttgatat gaagtggtgt tgggttgatc aagctggtgt gatatgtaag 3661 caagaaggaa tttttcgagt taattgcatg gactgcctgg atcgcaccaa cgtggtccaa 3721 gctgccattg cacgagtggt catggaacag cagcttaaaa aactaggtgt gatgccccct 3781 gagcagccac tacctgtgaa atgtaatcgg acctaccaga taatgtgggc taacaacggc 3841 gactccatca gcagacagta tgctggcaca gctgctctaa agggcgactt tacaagaaca 3901 ggagaaagga agttggcagg agttatgaaa gatggagtta actcagcaaa taggtactac 3961 ctcagccggt tcaaggatgc ttacaggcaa gctgtcatag atttgatgca aggcattcca 4021 gtgacggaag atctttattc catatttacc aaggagaagg agcatgaagc tttgcagaag 4081 gagacccaga gaagccacca agagctcatc agccagctct tacagagcta catgcagttg 4141 ctgctgccgg gggatgagaa gttccacggg ggctgggccc tcattgactg tgaccccagc 4201 ctcactgatg ctgctcacag agatgtggac gtgctactgc tgctttctaa ttctgcctac 4261 tatgtggcct attatgatga tgaagttgat aaagtaagcc agtatcaacg acggagcctt 4321 gaagacctgg aaaagataga aataggtccc gagcctactc tttttggcaa accaaagttc 4381 tcctgcatgc ggctgcacta cacgtgtaaa gaagcaggtg gttacttcca cacactgaga 4441 gccgtaccac gcagtcctga agaagacggg aaagatactc tccagtgcat tgcggagatg 4501 ctgcagatca ccaagcaggc catggggctg catgtgccca taatcgagag gaaactcgag 4561 aggaagagca gcaagcctca tgaagacatc attggtatca gatctcaaaa ccaaggctct 4621 ctggcccagg ggaaaagctt tttaatgagc aaattttcat ctctaaatca aaaagtaaag 4681 cagaccaaat ccaatgtgaa cattggcaac ctacgcaaat taggaaactt tacaaagcct 4741 gaagtgaaag tgaactttct aaaaccaaac ttaaaagtca acctttggaa atcagatagt 4801 agtcttgaga ccatggagaa cccaggagtg atgggtaaca aggtccaggg ggagtctgat 4861 ggggacgtgt cctcagataa tgactcttac cactctgatg aactccttac aaactccagg 4921 tctgaggaag gcaagcagct agccaactct ttggagagcg cagggccaac agactacctt 4981 ctcccgagct gtggcatcat cgcctcagcg cctcggttag gcagccggtc ccagtcggcc 5041 agcagcaccg acgttagcat ccatgctccc tcggaagcta ctgttggtca tggacatgag 5101 ctcggaaaag gcctggagtc tcccctgaag aaaagccctt ctgctgacaa catacacaca 5161 ttgactggct ttgcaaagcc catggacgtt tactgccaga gattcgtgca ggatgcacag 5221 aacaaaatga acgcgctgtc ggaggtcagg gctgtagctc agaacagcga ggagggaaat 5281 cacaagacta accctgtttc taatgaagaa acccagtcag aatcagtgga acagatgcct 5341 tctcggccct ctcagttaaa tgtctcttgt tctgtgacag gcccacagtt gttatcagtt 5401 gaaccagcac atccagcggt atctcagaag acccccggct ccgggtccag cctgctggac 5461 cttgaggcag ggctttgcgt aactccttct tcagagagca gcagcagagc agtctctccc 5521 tttgcaaaga tccgcagctc catggtccag gttgctaata ttacccaagc tgggctaact 5581 catgggataa acttggcagt tgccaaagtt cagaagagtc cagcagaacc cgaagtggtt 5641 aacgaaattc aacaaaatga acttaaaaat atgtttacac aatgccagac acgaataatt 5701 cagattCTCG AGTACCCATA CGACGTACCT GATTATGCAA CTTATCCTTA TGATGTACCT 5761 GATTATGCTA GCTATCCTTA TGATGTCCCA GACTACGCAT AGggatccCT GCGATATCCA 5821 GCgcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 5881 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 5941 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 6001 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 6061 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 6121 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 6181 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 6241 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 6301 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 6361 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 6421 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 6481 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 6541 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 6601 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 6661 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 6721 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 6781 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 6841 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 6901 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 6961 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 7021 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 7081 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 7141 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 7201 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 7261 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 7321 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 7381 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 7441 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 7501 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 7561 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 7621 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 7681 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 7741 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 7801 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 7861 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 7921 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 7981 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 8041 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 8101 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 8161 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 8221 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 8281 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 8341 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 8401 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 8461 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 8521 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 8581 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 8641 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 8701 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 8761 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 8821 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 8881 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 8941 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 9001 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 9061 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 9121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 9181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 9241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 9301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 9361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 9421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 9481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 9541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 9601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 9661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 9721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 9781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 9841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 9901 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 9961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 10021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 10081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 10141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 10201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 10261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 10321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 10381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 10441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 10501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 10561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 10621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 10681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 10741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 10801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 10861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 10921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 10981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 11041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 11101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 11161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 11221 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 11281 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 11341 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 11401 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 11461 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 11521 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 11581 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 11641 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 11701 agctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.