pLV2-SUSD2-3×FLAG-Puro plasmid

pLV2-SUSD2-3×FLAG-Puro plasmid

PVT97224

pLV2-SUSD2-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-SUSD2(human)-3×FLAG-Puro
Alias: NM_019601.4;BK65A6.2; W5C5;
Gene lenght: 2466bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-SUSD2-3×FLAG-Puro plasmid Description

pLV2-SUSD2-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-SUSD2-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgaagcc agccctcctg ccctgggccc tgctgctgct 2881 ggcgacagcc ctcggcccgg gccccggacc cacagcagat gcccaagaga gctgctccat 2941 gcgctgtggc gccctggacg ggccatgttc ctgccacccg acgtgctctg gccttggcac 3001 ctgctgcttg gatttccggg acttctgcct ggagatattg ccctactcag gatccatgat 3061 gggcggcaag gactttgtgg tgcggcactt caagatgtcc agccccacag acgccagtgt 3121 gatctgcagg tttaaggaca gcatccagac cctcggccat gtggactcct ccgggcaagt 3181 gcactgtgtg tcacctctgc tctatgagag cggccgcatc cccttcactg tgtcactgga 3241 caacggccac tccttccctc gtgcgggcac ctggctggct gtgcacccca acaaagtgtc 3301 aatgatggag aagagcgagt tggtgaacga gacgcgttgg caatactacg gcaccgccaa 3361 cacctcaggc aacctcagcc tgacctggca tgtcaagtcg ctgcccacgc agaccatcac 3421 catcgaactg tggggctacg aggagacagg aatgccctac tcacaggagt ggactgcaaa 3481 gtggtcgtac ctgtaccccc tggccacaca catccccaac tccggctctt tcactttcac 3541 cccaaaacct gctcctccca gctaccagag atggcgagtg ggtgcacttc ggatcatcga 3601 cagcaaaaat tacgcagggc agaaggacgt gcaggcgctc tggaccaacg accacgcact 3661 ggcctggcac ctgagcgatg acttccgaga ggaccctgtg gcctgggcac gaactcagtg 3721 ccaggcctgg gaggagctgg aggatcagct gcccaacttc ctggaggagc tgccggactg 3781 cccctgcacc ctgacccagg cccgggctga ctccggccgc ttcttcacgg actacggctg 3841 tgacatggag cagggcagcg tgtgcaccta ccaccccggg gccgtgcact gtgtgcgttc 3901 tgtgcaggcc agcctccggt acggctcagg tcagcagtgc tgctacacag cggacgggac 3961 gcagctcctg acagctgact ccagcggcgg cagcactccc gaccgcggcc atgactgggg 4021 cgcacccccg ttccgcacgc caccccgagt gcccagcatg tcccactggc tctacgatgt 4081 cctcagcttc tattactgct gcctctgggc acccgactgc ccccgctaca tgcaacggcg 4141 gccctccaat gactgccgca actaccggcc cccaagactg gcctccgcct tcggagaccc 4201 acactttgtg accttcgacg gcaccaactt cacattcaat gggcgcggag agtacgtgct 4261 gctggaggca gcgctgaccg acctgagggt gcaggcgcgg gcccagcccg ggacgatgtc 4321 caacggcacg gagacccgtg gcactgggct gaccgcagtg gccgtccagg agggcaactc 4381 agatgtggtg gaagtcaggc tggccaacag gaccggaggt ctggaggtgc tgctgaacca 4441 ggaggtgctg agcttcaccg agcagagctg gatggacctg aaaggaatgt tcctgtcggt 4501 ggctgccggg gacagggtct ccatcatgct ggcatcaggg gccggcctgg aggtcagcgt 4561 gcagggcccg ttcctgagtg tgtccgtcct gctgcctgag aagttcctca cccacaccca 4621 cggcctcctc gggacactca acaacgaccc caccgacgac ttcaccctgc acagcgggcg 4681 cgtcctgccc ccaggcacca gtccccagga gctgttcctg tttggggcca actggaccgt 4741 gcacaatgcg tcctccctgc tcacctacga ttcctggttc ctggtccaca acttcctgta 4801 ccaacccaag cacgacccca ccttcgagcc cctcttcccc agtgagacca ccctcaaccc 4861 cagcctggca caagaggcag ccaaactatg tggggacgat catttctgca actttgatgt 4921 ggcagccact gggagcctga gcacgggcac tgccactcgg gtggcccacc agctgcacca 4981 gcgtcgcatg cagagcctgc agccagtggt gtcctgtggc tggctggccc cacctcccaa 5041 cggacaaaag gagggcaaca ggtacctggc gggttccacc atctacttcc actgtgacaa 5101 cggctacagc ctggccgggg cagagaccag cacctgccag gctgacggca cctggtcctc 5161 acccaccccg aagtgccagc caggacgcag ctacgcggtg ctgttgggca tcatctttgg 5221 gggcctcgcg gtggtggcgg cggttgcgct cgtctatgtg ctgctgcgcc gcaggaaggg 5281 caacacgcac gtctggggtg cacagcccgg atccGACTAC AAAGACCATG ACGGTGATTA 5341 TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG tagtctagat aattctaccg 5401 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 5461 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 5521 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 5581 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 5641 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 5701 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 5761 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 5821 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 5881 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 5941 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 6001 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 6061 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 6121 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 6181 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 6241 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 6301 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 6361 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 6421 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 6481 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc 6541 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 6601 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 6661 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 6721 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 6781 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 6841 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 6901 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 6961 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 7021 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 7081 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt 7141 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat 7201 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta 7261 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 7321 aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc 7381 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 7441 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 7501 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 7561 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 7621 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 7681 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 7741 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 7801 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 7861 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 7921 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 7981 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca 8041 ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 8101 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 8161 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 8221 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 8281 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 8341 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 8401 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 8461 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 8521 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 8581 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 8641 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 8701 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 8761 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 8821 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 8881 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 8941 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 9001 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 9061 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 9121 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 9181 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 9241 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 9301 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 9361 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 9421 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 9481 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 9541 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 9601 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 9661 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 9721 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 9781 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 9841 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 9901 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 9961 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 10021 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 10081 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 10141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 10201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 10261 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 10321 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 10381 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 10441 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 10501 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 10561 gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.