pLV2-TRE3GS-Vps35-EGFP-TetOne-

pLV2-TRE3GS-Vps35-EGFP-TetOne-IRES-Puro plasmid

PVT97214

pLV2-TRE3GS-Vps35-EGFP-TetOne-IRES-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-Vps35(mouse)-EGFP-TetOne-IRES-Puro
Alias: NM_022997.5;Mem3
Gene lenght: 2388bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,Tetracycline induction
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction:

pLV2-TRE3GS-Vps35-EGFP-TetOne-IRES-Puro plasmid Description

pLV2-TRE3GS-Vps35-EGFP-TetOne-IRES-Puro is a plasmid suitable for protein expression in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV2-TRE3GS-Vps35-EGFP-TetOne-IRES-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg tCCATAGAGC CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC 4141 CTCCCCCTTG CTGTCCTGCC CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA 4201 ATGCGATGCA ATTTCCTCAT TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT 4261 CAAGGAAGGC ACGGGGGAGG GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA 4321 GGCTGATCAG CGGGTTTAAA ctaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt 4381 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg 4441 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact 4501 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg 4561 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt 4621 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac 4681 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc 4741 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg 4801 gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc gcgtggcggg gtagtcggcg 4861 aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt cgcgggtggc gaggcgcacc 4921 gtgggcttgt actcggtcat ggaaggtcgt ctccttgtgg gttgtggcaa gcttatcatc 4981 gtgtttttca aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc 5041 tcgactaaac acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg 5101 gtaccttctg ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc 5161 tttccacaac tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat 5221 cttatacacg tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc 5281 tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct 5341 tcacgacatt caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc 5401 tcgtcaagaa gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg 5461 tggaaaataa catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa 5521 cgttaggggg gggggaggga gaggggcgat tcgcgcgttt acccggggag catgtcaagg 5581 tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg 5641 gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac 5701 tttagctgtt tctccaggcc acatatgatt agttccaggc cgaaaaggaa ggcaggttcg 5761 gctccctgcc ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat agaatcggtg 5821 gtaggtgtct ctctttcctc ttttgctact tgatgctcct gttcctccaa tacgcagccc 5881 agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt tctccaggga gaagccttgc 5941 tgacacagga acgcgagctg attttccagg gtttcgtact gtttctctgt tgggcgggtg 6001 ccgagatgca ctttagcccc gtcgcgatgt gagaggagag cacagcggta tgacttggcg 6061 ttgttccgca gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc 6121 ctgtccagca tctcgattgg cagggcatcg agcagggccc gcttgttctt cacgtgccag 6181 tacagggtag gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt caggccttcg 6241 ataccgactc cattgagtaa ttccagagca gagtttatga ctttgctctt gtccagtcta 6301 gacatggtaa ttcgatgatc cccctgggga gagaggtcgg tgattcggtc aacgagggag 6361 ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac 6421 agggcccaca ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc 6481 tcggcgcgcc ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc 6541 attgctccct ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt 6601 cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc 6661 gtcgccgggg ggcccacaag ggtagcggcg aagatccggg tgacgctgcg aacggacgtg 6721 aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag 6781 ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa ccccgtggaa 6841 ttatcacctc gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta 6901 tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga 6961 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag 7021 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta 7081 tcagtgatag agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc 7141 tgattttttg agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc 7201 acttcctacc ctcgtaaaga attcGCCACC atgcctacaa cacagcagtc accccaggat 7261 gagcaggaaa aacttttgga tgaagccatc caggctgtga aggttcagtc attccagatg 7321 aaaagatgcc tggacaaaaa caagctgatg gatgctctga agcatgcctc caatatgctt 7381 ggagagctcc ggacctctat gctgtcacca aagagttact atgaacttta tatggctatt 7441 tctgatgaac tgcactactt ggaggtctac ctgactgatg aatttgctaa aggaagaaag 7501 gtggcagatc tctatgaact tgtacagtac gcgggaaaca ttattccaag gctttatctc 7561 ttgatcacag ttggagttgt atatgtcaag tcatttcctc aatccaggaa agatattttg 7621 aaagatttgg tagaaatgtg ccgtggtgtg cagcatccgc taaggggttt gtttcttcga 7681 aattaccttc ttcagtgtac taggaacatt ttacctgatg aaggagagcc aacagatgaa 7741 gaaacaactg gtgatatcag tgattccatg gattttgtac tactcaactt tgcagaaatg 7801 aataagctct gggtgcggat gcagcatcaa ggacatagtc gagatagaga aaaaagagaa 7861 cgagagagac aagaactgag aattttagtg ggaacaaatt tggtgcgcct tagtcagttg 7921 gaaggtgtaa atgtggaacg ttacaaacag attgttctaa caggcattct ggagcaggtt 7981 gtgaattgta gggatgctct ggctcaggag tatctcatgg agtgcatcat tcaggttttt 8041 cctgatgagt ttcatcttca gactttgaat ccttttctta gagcttgtgc tgaattacac 8101 caaaatgtaa atgtgaaaaa cataatcatt gctttaattg acagattagc tttatttgct 8161 catcgtgaag atggacccgg aattccagct gagattaaac tttttgacat attttcacaa 8221 caggtggcta cagtcataca gtccagacaa gacatgccat cagaggatgt tgtatcttta 8281 caagtctctc tcattaatct tgctatgaaa tgttaccctg atcgtgtgga ctacgtcgat 8341 aaagttctag aaacaacagt ggagatattc aataagctta accttgaaca tattgctacc 8401 agtagtgcag tttcaaagga gcttaccaga cttttgaaga tacctgttga tacttacaac 8461 aatatcttaa cagtattaaa gttaaagcat ttccacccac tttttgagta ctttgactac 8521 gagtccagaa agagcatgag ctgttatgtg cttagtaatg ttctggatta taacacagaa 8581 atcgtctctc aggaccaggt agattccata atgaatttgg tgtccacatt gattcaggat 8641 cagccagacc aacctgtaga agaccctgat ccagaagact tcgctgatga acagagcctt 8701 gttggccgat ttattcatct gctacgttct gatgatcctg accagcagta tttgattttg 8761 aatacggcac gaaaacattt tggggctggt ggaaatcagc ggattcgctt cacactgcca 8821 cctttggtat ttgcagctta ccagttggct tttcgataca aagagaattc ccaaatggat 8881 gacaagtggg aaaagaaatg ccagaagata ttttcatttg ctcaccagac tatcagtgct 8941 ttgattaaag ctgagctggc tgaattacca ctgagacttt ttcttcaagg agcattagct 9001 gctggagaaa ttggctttga aaatcatgaa acagtagcat atgaatttat gtcccaggca 9061 ttttctctat atgaagatga aatcagtgat tctaaagcac agctggctgc tatcaccttg 9121 atcatcggta cttttgagag gatgaaatgc ttcagtgaag agaatcatga acccttgaga 9181 actcagtgtg cacttgctgc atcaaaactt ctgaaaaaac cagatcaagg ccgagccgtg 9241 agcacatgtg cgcatctctt ttggtctggc cgaaacacag acaaaaatgg ggaagagctt 9301 catggaggta aaagggtcat ggagtgccta aagaaggcac taaaaatagc aaatcagtgc 9361 atggacccct ctctacaagt tcagctcttt atagagattc tgaacaggta catctatttc 9421 tatgaaaaag aaaatgatgc ggtaaccatt caagtcttga accaacttat tcaaaagatt 9481 cgagaagatc tcccaaatct tgagtccagt gaagaaacag aacaaataaa caagcatttt 9541 cacaacacat tggagcactt gcgctcaaga cgggaatcac cagagtctga ggggccaatc 9601 tatgaaggtc tcatccttgg tggaggcgga tcaggaggcg gtggatctgg atccgccacc 9661 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 9721 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 9781 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 9841 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 9901 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 9961 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 10021 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 10081 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 10141 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 10201 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 10261 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 10321 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtag 10381 agaccacctc ccctgcgagc taagctggac agccaatgac gggtaagaga gtgacatttt 10441 tcactaacct aagacaggag ggccgtcaga gctactgcct aatccaaaga cgggtaaaag 10501 tgataaaaat gtatcactcc aacctaagac aggcgcagct tccgagggat ttgagatcca 10561 gacatgataa gatacattga tgagtttgga caaaccaaaa ctagaatgca gtgaaaaaaa 10621 tgccttattt gtgaaatttg tgatgctatt gccttatttg taaccattat aagctgcaat 10681 aaacaagtta agtcgataaa ctggatctct gctgtccctg taataaaccc gaaaattttg 10741 aatttttgta atttgttttt gtagttcttt agtttgtatg tctgttgcta ttatgtctac 10801 tattctttcc cctgcactgt accccccaat cccccctttt cttttaaagg cgataccgtc 10861 gagatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta 10921 ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg 10981 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta 11041 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa 11101 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt 11161 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc 11221 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac 11281 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca 11341 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga 11401 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt 11461 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg 11521 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca 11581 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg 11641 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc 11701 ctcctccagg tctgaagatc agcggccggc cgcttgctgt gcggtggtct tacttttgtt 11761 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg 11821 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg 11881 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc 11941 aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata 12001 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg 12061 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt 12121 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt 12181 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca 12241 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca 12301 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc 12361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac 12421 ccagtacagg caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt 12481 cccgcccagg ccacgcctcc ctggaaagtc cccagcggaa agtcccttgt agcaagctcg 12541 atatcagcag ttcttgaagt actccggatg cagctctcgg gccacgtgat gaaatgctag 12601 gcggctgtca aacctccact ctaacacttc tctctccggg tcatccatcc catgcaggct 12661 cacagggtgt aacaagctgg tgttctctcc tttattggcc tcttctacct tatctggctc 12721 aactggtact agcttgtagc accatccaaa ggtcagtgga tatctgaccc ctggccctgg 12781 tgtgtagttc tgctaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat 12841 atcttgtctt ctttgggagt gaattagccc ttcca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.