pLV3-CMV-LAMTOR1-3×FLAG-CopGFP

pLV3-CMV-LAMTOR1-3×FLAG-CopGFP-Puro plasmid

PVT97203

pLV3-CMV-LAMTOR1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-LAMTOR1(human)-3×FLAG-CopGFP-Puro
Alias: NM_017907.3;C11orf59; p18; p27RF-Rho; PDRO; Ragulator1
Gene lenght: 483bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LAMTOR1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-LAMTOR1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-LAMTOR1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ggtgctgcta cagcagcgag 2341 aacgaggact cggaccagga ccgagaggag cggaagctgc tgctggaccc tagcagcccc 2401 cctaccaaag ctctcaatgg agccgagccc aactaccaca gcctgccttc cgctcgcact 2461 gatgagcagg ccctgctctc ttccatcctt gccaagacag ccagcaacat cattgatgtg 2521 tctgctgcag actcacaggg catggagcag catgagtaca tggaccgtgc caggcagtac 2581 agcacccgct tggctgtgct gagcagcagc ctgacccatt ggaagaagct gccaccgctg 2641 ccgtctctta ccagccagcc ccaccaagtg ctggccagtg agcccatccc gttctctgat 2701 ttgcagcagg tctccaggat agctgcttat gcctacagtg cactttctca gatccgtgtg 2761 gacgcaaaag aggagctggt tgtacagttt gggatcccag gatccGACTA CAAAGACCAT 2821 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 2881 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 2941 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 3001 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 3061 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 3121 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 3181 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 3241 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 3301 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 3361 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 3421 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 3481 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 3541 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 3601 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 3661 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 3721 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 3781 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 3841 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 3901 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 3961 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 4021 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 4081 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 4141 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 4201 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 4261 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 4321 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 4381 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 4441 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 4501 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 4561 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 4621 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 4681 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 4741 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 4801 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 4861 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 4921 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 4981 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5041 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 5101 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 5161 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 5221 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 5281 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 5341 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 5401 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 5461 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 5521 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 5581 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 5641 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 5701 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 5761 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 5821 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 5881 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 5941 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 6001 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6061 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 6121 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 6181 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 6241 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 6301 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6361 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6421 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6481 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6541 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6601 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6661 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6721 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6781 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6841 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6901 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6961 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7021 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7081 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7141 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7201 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 7261 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7321 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7381 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7441 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 7501 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 7561 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7621 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7681 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7741 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7801 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 7861 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 7921 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 7981 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8041 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8101 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 8161 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 8221 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 8281 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 8341 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 8401 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 8461 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 8521 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 8581 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 8641 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 8701 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 8761 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.