pLV3-CMV-HIF1A-AS3-CopGFP-Puro

pLV3-CMV-HIF1A-AS3-CopGFP-Puro plasmid

PVT97185

pLV3-CMV-HIF1A-AS3-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HIF1A-AS3(human)-CopGFP-Puro
Alias: NR_144368.1
Gene lenght: 533bp
Host: Mammalian cells,lentivirus
Use(s): RNATranscription
Fragment type: ncRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HIF1A-AS3-CopGFP-Puro plasmid Description

pLV3-CMV-HIF1A-AS3-CopGFP-Puro A plasmid suitable for transcriptional regulation in mammalian cells, also applicable for lentivirus packaging.

pLV3-CMV-HIF1A-AS3-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattctcat gtagcgccag ccacaccagg aacagaaggg tgccgggtac 2341 cttccgcatg cttggtattc tccccgcggg gctctgaccg ctgccgctct caggcacctg 2401 tctttcctct ccgtcccaga atggagccaa gacaagggaa taaacgaaat tcaatagtac 2461 acggagatcg ggtgtctggg cagcgtcttg gaaaaactat ccactacagt acaggtcaag 2521 tgaagttctt ctgctgatat gttctgctac tgcataagag acggaatcta tgttgcctcg 2581 actggtcctg aactcctggg ctaaagcgat ccttctgcct tggcctccca aagtgcgagg 2641 attataggtg tgagccaccc aacctggcct ttgccagtat ttttaaatcc aaacacctat 2701 gcatggtgct tactaataac acagtggtat tctaaacatt tttcacctat tatttaatct 2761 ttattattat caccatgtta cagataagga ccctggctaa atagcttacc caacattact 2821 ccgttaatag gatccgcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc 2881 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg 2941 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 3001 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 3061 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc 3121 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc 3181 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc 3241 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac 3301 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg 3361 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag 3421 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt 3481 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa 3541 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg 3601 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa 3661 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt 3721 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac 3781 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt 3841 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag 3901 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc 3961 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga 4021 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat 4081 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc 4141 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga 4201 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 4261 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 4321 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 4381 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 4441 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 4501 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 4561 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 4621 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 4681 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 4741 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 4801 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 4861 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 4921 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 4981 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 5041 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 5101 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 5161 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 5221 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 5281 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 5341 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc cgcctccccg 5401 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 5461 agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc tgctttttgc 5521 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5581 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5641 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5701 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 5761 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 5821 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 5881 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 5941 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 6001 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 6061 ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 6121 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 6181 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 6241 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 6301 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 6361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 6421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 6481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 6541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 6601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 6661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 6721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 6781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 6841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 6901 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 6961 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 7021 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 7081 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 7141 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 7201 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 7261 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 7321 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 7381 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 7441 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 7501 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 7561 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 7621 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 7681 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 7741 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 7801 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 7861 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 7921 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 7981 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 8041 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 8101 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 8161 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 8221 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 8281 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 8341 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 8401 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 8461 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 8521 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 8581 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 8641 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 8701 acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.