pLV3-CMV-KIAA0232-circRNA-CopG

pLV3-CMV-KIAA0232-circRNA-CopGFP-Puro plasmid

PVT97158

pLV3-CMV-KIAA0232-circRNA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-KIAA0232(human)-circRNA-CopGFP-Puro
Alias: -
Gene lenght: 638bp
Host: Mammalian cells,lentivirus
Use(s): Transposition regulation
Fragment type: circRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KIAA0232-circRNA-CopGFP-Puro plasmid Description

pLV3-CMV-KIAA0232-circRNA-CopGFP-Puro A plasmid suitable for transcriptional regulation in mammalian cells, also applicable for lentivirus packaging.

pLV3-CMV-KIAA0232-circRNA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagatTA 2281 ATACTTTCAG aattcGCCAC CGTTCTGCCG GAGACTTCCA TTTGGCCTCA ATGTGAAATT 2341 AAAGTAGAAA ATCACATCTA CATGCATGTT GCTATCAGGA TGTTGATTCA TTAGTCATGC 2401 CTGAAGAGGG AAAGTCTGTT TTAGGTGGCT GACTACCAGC AAAAATAAAT GCTTATCCTA 2461 CATGTCAAGC ATCTCTACTT TTTACTGGAG TGAAAATCCC CTCCAGATAC CAACAGAATA 2521 TCAACTGCAG AAAATGCTTC ACATTTTAAG GATGTCGGCA ACCTAAATTC ATGTACCCTA 2581 TCTGTACAGT TGTTGTGGAT GGTTTGCCAT CTGAAAGCTC CTCAAGTTCT TATCCAGGCC 2641 CTGTGTCTGT TTCTGAAATG TCTCTGCTTC ATGCTTTGGG TCCAGTGCAG ACCTGGCTGG 2701 GACAAGAGCT CGAGAAATGT GGCATTGATG CCATGATTTA CACTCGGTAT GTCCTCAGTC 2761 TTCTGCTGCA TGACAGCTAT GACTACGACC TGCAGGAACA GGAGAATGAC ATCTTCCTGG 2821 GCTGGGAAAA AGGAGCTTAT AAGAAATGGG GAAAGAGTAA GAAAAAATGT TCAGATCTAA 2881 CTCTAGAAGA AATGAAAAAA CAGGCTGCTG TCCAGTGTCT TCGATCTGCT TCTGATGAAC 2941 TCGAGTAAGA ACAACTGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 3001 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 3061 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 3121 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 3181 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 3241 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 3301 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 3361 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 3421 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 3481 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 3541 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 3601 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 3661 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 3721 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 3781 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 3841 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 3901 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 3961 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 4021 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 4081 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 4141 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 4201 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 4261 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 4321 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 4381 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 4441 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 4501 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 4561 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 4621 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 4681 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 4741 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 4801 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 4861 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 4921 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 4981 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 5041 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 5101 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 5161 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 5221 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 5281 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 5341 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 5401 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 5461 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta 5521 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 5581 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact 5641 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 5701 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 5761 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 5821 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 5881 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 5941 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 6001 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg 6061 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 6121 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca 6181 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 6241 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 6301 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 6361 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 6421 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 6481 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 6541 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 6601 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 6661 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 6721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 6781 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 6841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 6901 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 6961 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 7021 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 7081 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 7141 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 7201 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 7261 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 7321 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 7381 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 7441 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 7501 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 7561 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 7621 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 7681 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 7741 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 7801 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 7861 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 7921 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 7981 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 8041 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 8101 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 8161 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 8221 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 8281 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 8341 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 8401 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 8461 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 8521 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 8581 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 8641 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 8701 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 8761 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 8821 gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.