pCMV-Spata31d1e-3×FLAG-Neo pla

pCMV-Spata31d1e-3×FLAG-Neo plasmid

PVT97140

pCMV-Spata31d1e-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Spata31d1e(mouse)-3×FLAG-Neo
Alias: XM_017315672.3
Gene lenght: 2949bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Spata31d1e-3×FLAG-Neo plasmid Description

pCMV-Spata31d1e-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Spata31d1e-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatgaa aaagaaaatg tctccccaga 781 ctctggagga ggtgaaaaag agggatgaca tttctccaga tcccaagagg tcagaaggca 841 aactcacagc cctgtggaat tcgcagggtt cgactgtcac taacctagac acccaggtcc 901 caaaaatcaa cttgaagctg aaagggaaac ggaagtattt tggggcttat gaccaggtcc 961 tgtatgtgaa gatcccagag ggagacctgg gccagaaaca cagccagctc ttctggggtc 1021 tcccctctct gcacagtgag tccatcatgg ccactctcct ggttcctgcg agcagctact 1081 caccagagcc ttgccttgtg ctgttcaatg gggtctgtaa aacagcttca gccccagtgc 1141 tgagtcatgg tcttccagcc cttccccagc ccctcaccct ggtccatcct caacctttgc 1201 ctaaggtagc tccccagcca cagactctta ctgagggaat gccccaggtc cacatgcaat 1261 ctccattgtc ttccatggcc ttgccatctc taccctgtgg gagtgcactt cagataccac 1321 agaccaggac cgtctctgac gtcttaaatg ggaaaggaca cttgcaatat cacttggtgc 1381 agaatcctca agacagtttg tggggtttgg tccctgagtg ccagcaatat gaaactgccc 1441 ttggtcttcc agttcacagc tttcccgtgg gcagccagtc ttcacagacc tatgtctccg 1501 tccccgggtc tggccttttt catttcagca gtgaacatca gaacaacctg gacccctatg 1561 gaccaaacaa cctaacctca ccctcgtgct tcgagctgtg caataggcca ggagtgccag 1621 aagtgatgaa cactcagttc aatccaacag gagtatcttc atactgctac aaatacgcta 1681 cgtctcaatg ctgcgtacac tggggcaaca tctgtgctga ccctgggaat ggagaactaa 1741 gtcactcgga gagttattgc gtgaaggtcc ccatgaagcc acagctaaga aaggatgcag 1801 ccaagaacct tggccagatc ctagggaaat gcccattaga caacacacaa atgatctcag 1861 gatgttgtat actgaatggt ttgaagacta tccctgagac agagggggag tcagtgtgtc 1921 actcaaggac tagcttggaa aatgaacagc tcaccatctc aaagaagaac ttggaccaaa 1981 gacacatgag aagcattctg aggttgcaca tgagcaggaa gtgctggcag atcactatgg 2041 gtcggattcc aatcgtggtg tgttgttcat ggctcgatga agatgcgtct ctctggagat 2101 cacccctggg aaagattcac tgtgatactg ctattccaga aatacctttt cttgatcaca 2161 agacccagaa gatgctggaa gcccatctca taaggttccg tgtgagtcag caatggggcc 2221 tccccatcaa ggttattgaa tccataaaat tctatatttt gagagaagca aaaacatggc 2281 cccttcctca gtctgatctc cccttatcat taaacagtat tcctgggttg gatttgaaaa 2341 gtaactttcc cagtcccctc aggggaagct ccgaccttct ccatggagac aagatagaaa 2401 ccacagactc agcccccacc acagattact ctctcaccct cttacatgta gacagcgaag 2461 gagagaggtc cctgagacag tcagtctcta gccccacctc ggatgtttca gagacggttg 2521 aggatgacag gcccagcaac ccagacctga gccagaacga gactgatcta caaagcagac 2581 ccagacagga gcagcccgta aaggaagacg tgactctgtc tgactcagag aatgagagcg 2641 caagttctgc ttctcatcta gtaactgctg aaagcagaca gagtgtagaa aagaatccaa 2701 aatacacggt gacacccaac atgctcagag agatgatact caaggccagg gagctgtttg 2761 ttcggtcagg gtctgattct ataatcaagt caaaaagttc caccaaaaaa gacgaagatg 2821 agagtctatc aagccccctg gtggccacag ggcagagccc atcaaagatg tcagtccctg 2881 aagagccaac agcaccagac ttgtttaagg aactaaggtc gaagttggag gatcagccac 2941 agagccagag tgaagcctgg gaggacgagt catcctttgt ctcagacagc ttctcgctgc 3001 cgtcttcttc caacagcgtg tctagtgcag acgtctccgt cttctgggac acacaaggcc 3061 acatgtatag caccggaatc agtgtggatc cgtggcagga gcccagtgtc ttcaaaagat 3121 tcttaaagaa tgtcatgcca gctcagaaga gactgtcgtt gcccaaatcc agaaaagaaa 3181 atggtagaag cgaccctcag gaattagaga cacccaaagc aggaaagaag agccaaccta 3241 gtaaggagaa atcaagcaag agggaacatc ctcctgacag ttacttcaga aaaaagatag 3301 gacaattttt ccagtggctt tattccagca aagacagccc aagacataga tcggagaaag 3361 acggggctct ctttatgagc tgcgggcccc cagaagctca cgaactcatg gcatcccttg 3421 ggaaactcct ggaagataag ctgttgtatg ggcagaactc cgagttcttg gagtggagcc 3481 agacgagcat gctcgctcag ccagcgctca agggacagcc ttccgacctt ggggctgccg 3541 atggccatca tggagaagaa gcgagtaatt gctcctgtcc ccagacagcc atcacccctg 3601 gtccaagtca tatgctgagc cttggacaga gacatcatcc cctacctttt gagtaccctc 3661 cctctgctcc ggagagtctg agttctgtgc aagctccgct tgcaGGATCC GACTACAAAG 3721 ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGT 3781 GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 3841 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 3901 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 3961 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 4021 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 4081 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 4141 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC 4201 GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 4261 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 4321 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 4381 CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA 4441 ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT 4501 TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA 4561 TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA 4621 AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG 4681 CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 4741 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 4801 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 4861 CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 4921 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 4981 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 5041 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA 5101 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG 5161 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 5221 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 5281 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 5341 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 5401 GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 5461 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 5521 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 5581 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 5641 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 5701 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 5761 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 5821 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 5881 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 5941 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 6001 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 6061 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 6121 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 6181 GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 6241 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 6301 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT 6361 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA 6421 TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG 6481 GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA 6541 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 6601 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG 6661 CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 6721 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 6781 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 6841 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 6901 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 6961 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 7021 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 7081 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 7141 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 7201 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 7261 AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 7321 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 7381 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 7441 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 7501 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 7561 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 7621 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 7681 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 7741 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 7801 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 7861 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 7921 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 7981 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 8041 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 8101 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 8161 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 8221 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 8281 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 8341 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 8401 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 8461 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 8521 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 8581 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 8641 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 8701 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 8761 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 8821 AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC 8881 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG 8941 GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC 9001 AATTGCATGA AGAATCTGCT TAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.