pCMV-Foxp1-T2A-EYFP-Neo plasmi

pCMV-Foxp1-T2A-EYFP-Neo plasmid

PVT97125

pCMV-Foxp1-T2A-EYFP-Neo plasmid Information

Plasmid name: pCMV-Foxp1(mouse)-T2A-EYFP-Neo
Alias: NM_053202.2;3110052D19Rik; 4932443N09Rik
Gene lenght: 2115
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Yellow
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-EYFP-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Foxp1-T2A-EYFP-Neo plasmid Description

pCMV-Foxp1-T2A-EYFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Foxp1-T2A-EYFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaacgg gccctctaga ctcgagGCCA CCatgatgca agaatctggg tctgagacaa 961 aaagtaacgg atcagccatc cagaacgggt ccagcggtgg caaccactta ctagagtgcg 1021 gggcacttcg tgacactcgg tccaacggag aggcaccagc ggtggacctg ggggcagccg 1081 accttgccca cgtccagcag cagcaacagc aggccctgca ggtggcaagg cagctcctcc 1141 ttcagcagca gcaacagcag cagcagcaac agcagcagca gcagcagcag caacagcagc 1201 aacagcagca gcaacaacag cagcagcagc aacagcagca gcagcagcag caagttagtg 1261 gattaaagtc tcccaagagg aatgacaagc aaccagctct tcaggttccc gtgtcagtgg 1321 ctatgatgac acctcaggtt atcactcctc aacaaatgca gcagatcctc cagcagcagg 1381 tgctgagccc ccagcagctc caggttctcc tccagcagca gcaggccctc atgcttcaac 1441 agcagcttca agaattttat aaaaaacaac aggaacagtt gcagcttcaa cttctccaac 1501 agcaacatgc tggaaaacag ccgaaagagc agcaggtggc tacccagcag ttggctttcc 1561 agcagcagct tctccagatg cagcagctgc agcagcagca cctcttgtct ctccagcgcc 1621 aaggcctcct aacaattcag ccggggcagc ctgcccttcc cctccaaccc ctcgctcaag 1681 gcatgattcc aacggaactg cagcagctct ggaaagaagt gacaagtgcc cacactgcag 1741 aggaaaccac aagcagcaac cacagcagcc tagacctgac cagcacatgt gtctcgtcct 1801 cggcaccttc caagtcctcc ctaatcatga acccgcatgc ctctaccaat ggacagctct 1861 cggtccacac tcccaaaagg gaaagcttgt cccacgagga gcacccccac agccaccctc 1921 tctatggaca tggcgtatgc aagtggccag gctgtgaggc ggtttgtgac gacttcccag 1981 cctttctaaa acatctcaac agtgagcatg cgctggacga tagaagcaca gctcaatgta 2041 gagtacaaat gcaggttgta cagcagttag agctacagct tgcaaaagac aaagagcgcc 2101 tgcaagccat gatgacccac ctgcatgtga agtctacaga acccaaagct gcccctcagc 2161 ccctgaatct ggtatcaagt gtcaccctct ccaagtctgc ctcagaggct tctccacaga 2221 gcttacctca tactccaaca acccccaccg cccccctgac tcctgtcacc caaggcccct 2281 ccgtcatcac caccaccagc atgcacacgg tgggacctat ccgcaggcgg tactcagaca 2341 aatacaacgt gcccatttct tcagcagata ttgcgcagaa ccaagaattt tataagaacg 2401 cggaagttag accaccattt acatatgcat ctttaatcag gcaggccatt ctcgaatctc 2461 cagaaaagca gctaacacta aacgaaatct ataactggtt cacacgaatg tttgcttact 2521 tccgacgcaa tgcagccacg tggaagaatg cagtgcgtca taatcttagt ctccacaagt 2581 gttttgtgcg agtagagaac gttaaagggg cagtatggac agtggatgaa gtagagttcc 2641 aaaaacggag gccacaaaag atcagtggta acccttccct tattaaaaac atgcagagca 2701 gccacgccta ctgcacacct ctcaatgcag ctttacaggc ttccatggct gagaatagta 2761 tacctctgta cactaccgct tccatgggaa atcccactct gggcagcctg gccagtgcca 2821 tccgggagga gctgaacggg gccatggagc acaccaacag caacgagagt gacagcagtc 2881 caggcagatc ccctatgcaa gctgtgcacc ccatacacgt caaagaagaa cccctcgacc 2941 ccgaggaagc tgaaggccct ctgtccttag tgacaacagc caaccacagt ccagattttg 3001 accatgacag agattacgaa gacgaaccag taaatgagga catggagggt tcaggcgagg 3061 gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca gatatcatgg 3121 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 3181 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 3241 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 3301 tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc 3361 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 3421 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 3481 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 3541 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 3601 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 3661 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 3721 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 3781 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagagc 3841 tcggtaccaa gcttgggccc gaacaaaaac tcatctcaga agaggatctg aatagcgccg 3901 tcgaccatca tcatcatcat cattgagttt aaacggtctc cagcttaagt ttaaaccgct 3961 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 4021 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 4081 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 4141 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 4201 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 4261 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 4321 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 4381 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 4441 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 4501 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 4561 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 4621 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 4681 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 4741 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 4801 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 4861 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 4921 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 4981 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 5041 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 5101 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 5161 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 5221 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 5281 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 5341 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 5401 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 5461 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 5521 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 5581 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 5641 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 5701 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 5761 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 5821 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 5881 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 5941 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 6001 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 6061 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 6121 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 6181 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 6241 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 6301 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 6361 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 6421 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 6481 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 6541 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 6601 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 6661 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6721 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6781 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 6841 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 6901 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 6961 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 7021 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 7081 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 7141 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 7201 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 7261 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 7321 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 7381 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 7441 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 7501 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 7561 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 7621 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 7681 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 7741 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 7801 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 7861 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 7921 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 7981 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 8041 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 8101 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 8161 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 8221 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 8281 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 8341 ccgcgcacat ttccccgaaa agtgccacct gacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.