pU6-HMMR-sgRNA3-Cas9-EGFP-Puro

pU6-HMMR-sgRNA3-Cas9-EGFP-Puro plasmid

PVT97112

pU6-HMMR-sgRNA3-Cas9-EGFP-Puro plasmid Information

Plasmid name: pU6-HMMR(human)-sgRNA3-Cas9-EGFP-Puro
Alias: CGTGTTCTTCTACAGGAACG
Gene lenght:
Host: Mammalian cells
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pU6-HMMR-sgRNA3-Cas9-EGFP-Puro plasmid Description

pU6-HMMR-sgRNA3-Cas9-EGFP-Puro is a plasmid suitable for gene editing in mammalian cells.

pU6-HMMR-sgRNA3-Cas9-EGFP-Puro plasmid Sequence

1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga 241 cgaaacaccg gCGTGTTCTT CTACAGGAAC Ggttttagag ctagaaatag caagttaaaa 301 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag 361 agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg ccaattctgc 421 agacaaatgg ctctagaggt acccgttaca taacttacgg taaatggccc gcctggctga 481 ccgcccaacg acccccgccc attgacgtca atagtaacgc caatagggac tttccattga 541 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 601 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc 661 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 721 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 781 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg 841 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg 901 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg 961 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg 1021 acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg 1081 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 1141 ttagctgagc aagaggtaag ggtttaaggg atggttggtt ggtggggtat taatgtttaa 1201 ttacctggag cacctgcctg aaatcacttt ttttcaggtt ggaccggtgc caccatggac 1261 tataaggacc acgacggaga ctacaaggat catgatattg attacaaaga cgatgacgat 1321 aagatggccc caaagaagaa gcggaaggtc ggtatccacg gagtcccagc agccgacaag 1381 aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac 1441 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc 1501 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg 1561 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa 1621 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag 1681 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg 1741 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg 1801 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag 1861 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag 1921 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc 1981 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa 2041 aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc 2101 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa 2161 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc 2221 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc 2281 gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag 2341 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg 2401 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt 2461 gacggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg 2521 gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg 2581 accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg 2641 cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc 2701 ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc 2761 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac 2821 aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc 2881 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag 2941 ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag 3001 cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag 3061 ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg 3121 gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac 3181 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg 3241 acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc 3301 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc 3361 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg 3421 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc 3481 tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac 3541 attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg 3601 gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg 3661 gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg 3721 atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac 3781 acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac 3841 gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca tatcgtgcct 3901 cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac 3961 cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg 4021 cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc 4081 gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa 4141 acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac 4201 gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg 4261 tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac 4321 gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag 4381 ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc 4441 aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg 4501 aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc 4561 gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg 4621 cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc 4681 ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag 4741 aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg 4801 ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg 4861 ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa 4921 gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg 4981 ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga 5041 aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag 5101 aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag 5161 cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc 5221 gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga 5281 gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc 5341 ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg 5401 gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct 5461 cagctgggag gcgacaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa 5521 aaggaattcg gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag 5581 aatcctggcc cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 5641 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 5701 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 5761 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 5821 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 5881 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 5941 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 6001 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 6061 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 6121 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 6181 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 6241 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 6301 tacaaggaat tctaactaga gctcgctgat cagcctcgac tgtgccttct agttgccagc 6361 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 6421 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 6481 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg 6541 ctggggagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc 6601 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg 6661 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc 6721 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg 6781 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 6841 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 6901 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 6961 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg 7021 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 7081 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt 7141 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 7201 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc 7261 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg 7321 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagGT GTGTCAGTTA 7381 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 7441 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 7501 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 7561 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 7621 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 7681 GGCCTAGGCT TTTGCAAAAA GCTCCCggga tgACCGAGTA CAAGCCCACG GTGCGCCTCG 7741 CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC 7801 CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG 7861 AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG 7921 CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA 7981 TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG 8041 GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGAGTCT 8101 CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG 8161 CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT 8221 ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT 8281 GGTGCATGAC CCGCAAGCCC GGTGCCTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG 8341 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 8401 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 8461 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 8521 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA acgcgcgaga cgaaagggcc 8581 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 8641 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 8701 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 8761 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 8821 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 8881 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 8941 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 9001 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 9061 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 9121 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 9181 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 9241 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 9301 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 9361 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 9421 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 9481 gtggaagccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 9541 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 9601 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 9661 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 9721 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 9781 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 9841 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 9901 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 9961 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 10021 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 10081 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 10141 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 10201 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 10261 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 10321 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 10381 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 10441 ctcacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.