pLV3-U6-MIR21-5p-shRNA1-CopGFP

pLV3-U6-MIR21-5p-shRNA1-CopGFP-Puro plasmid

PVT97060

pLV3-U6-MIR21-5p-shRNA1-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-MIR21(human)-5p-shRNA1-CopGFP-Puro
Alias: tag cttatcagac tgatgttga
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-MIR21-5p-shRNA1-CopGFP-Puro plasmid Description

pLV3-U6-MIR21-5p-shRNA1-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-MIR21-5p-shRNA1-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgaattc Gtagcttatc agactgatgt tgaTTCAAGA 2221 GAtcaacatc agtctgataa gctaTTTTTT ggatccgcgg ccgcGaagga tctgcgatcg 2281 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 2341 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 2401 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 2461 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 2521 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 2581 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 2641 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 2701 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 2761 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc 2821 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg 2881 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg 2941 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct 3001 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa 3061 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga 3121 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg 3181 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat 3241 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg 3301 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag 3361 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt 3421 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca 3481 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc 3541 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct 3601 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg 3661 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 3721 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 3781 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 3841 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 3901 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 3961 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 4021 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 4081 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 4141 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 4201 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg 4261 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 4321 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 4381 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 4441 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 4501 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 4561 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 4621 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 4681 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 4741 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 4801 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 4861 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 4921 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 4981 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 5041 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 5101 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 5161 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 5221 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 5281 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 5341 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 5401 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 5461 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 5521 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 5581 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 5641 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 5701 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 5761 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 5821 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 5881 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 5941 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 6001 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 6061 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 6121 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 6181 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 6241 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 6301 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 6361 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6421 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 6481 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 6541 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 6601 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 6661 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 6721 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 6781 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 6841 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 6901 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 6961 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 7021 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 7081 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 7141 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 7201 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 7261 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 7321 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 7381 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 7441 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 7501 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 7561 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 7621 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 7681 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 7741 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 7801 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 7861 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 7921 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 7981 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 8041 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 8101 cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.