pLV3-CMV-CREBBP-3×FLAG-Hyg pla

pLV3-CMV-CREBBP-3×FLAG-Hyg plasmid

PVT96878

pLV3-CMV-CREBBP-3×FLAG-Hyg plasmid Information

Plasmid name: pLV3-CMV-CREBBP(human)-3×FLAG-Hyg
Alias: NM_004380.3;CBP; KAT3A; MKHK1; RSTS; RSTS1
Gene lenght: 7326bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CREBBP-3×FLAG-Hyg plasmid Description

pLV3-CMV-CREBBP-3×FLAG-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CREBBP-3×FLAG-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcc GGATCCGCCA CCatggctga gaacttgctg gacggaccgc ccaaccccaa 2341 aagagccaaa ctcagctcgc ccggtttctc ggcgaatgac agcacagatt ttggatcatt 2401 gtttgacttg gaaaatgatc ttcctgatga gctgataccc aatggaggag aattaggcct 2461 tttaaacagt gggaaccttg ttccagatgc tgcttccaaa cataaacaac tgtcggagct 2521 tctacgagga ggcagcggct ctagtatcaa cccaggaata ggaaatgtga gcgccagcag 2581 ccccgtgcag cagggcctgg gtggccaggc tcaagggcag ccgaacagtg ctaacatggc 2641 cagcctcagt gccatgggca agagccctct gagccaggga gattcttcag cccccagcct 2701 gcctaaacag gcagccagca cctctgggcc cacccccgct gcctcccaag cactgaatcc 2761 gcaagcacaa aagcaagtgg ggctggcgac tagcagccct gccacgtcac agactggacc 2821 tggtatctgc atgaatgcta actttaacca gacccaccca ggcctcctca atagtaactc 2881 tggccatagc ttaattaatc aggcttcaca agggcaggcg caagtcatga atggatctct 2941 tggggctgct ggcagaggaa ggggagctgg aatgccgtac cctactccag ccatgcaggg 3001 cgcctcgagc agcgtgctgg ctgagaccct aacgcaggtt tccccgcaaa tgactggtca 3061 cgcgggactg aacaccgcac aggcaggagg catggccaag atgggaataa ctgggaacac 3121 aagtccattt ggacagccct ttagtcaagc tggagggcag ccaatgggag ccactggagt 3181 gaacccccag ttagccagca aacagagcat ggtcaacagt ttgcccacct tccctacaga 3241 tatcaagaat acttcagtca ccaacgtgcc aaatatgtct cagatgcaaa catcagtggg 3301 aattgtaccc acacaagcaa ttgcaacagg ccccactgca gatcctgaaa aacgcaaact 3361 gatacagcag cagctggttc tactgcttca tgctcataag tgtcagagac gagagcaagc 3421 aaacggagag gttcgggcct gctcgctccc gcattgtcga accatgaaaa acgttttgaa 3481 tcacatgacg cattgtcagg ctgggaaagc ctgccaagtt gcccattgtg catcttcacg 3541 acaaatcatc tctcattgga agaactgcac acgacatgac tgtcctgttt gcctcccttt 3601 gaaaaatgcc agtgacaagc gaaaccaaca aaccatcctg gggtctccag ctagtggaat 3661 tcaaaacaca attggttctg ttggcacagg gcaacagaat gccacttctt taagtaaccc 3721 aaatcccata gaccccagct ccatgcagcg agcctatgct gctctcggac tcccctacat 3781 gaaccagccc cagacgcagc tgcagcctca ggttcctggc cagcaaccag cacagcctca 3841 aacccaccag cagatgagga ctctcaaccc cctgggaaat aatccaatga acattccagc 3901 aggaggaata acaacagatc agcagccccc aaacttgatt tcagaatcag ctcttccgac 3961 ttccctgggg gccacaaacc cactgatgaa cgatggctcc aactctggta acattggaac 4021 cctcagcact ataccaacag cagctcctcc ttctagcacc ggtgtaagga aaggctggca 4081 cgaacatgtc actcaggacc tgcggagcca tctagtgcat aaactcgtcc aagccatctt 4141 cccaacacct gatcccgcag ctctaaagga tcgccgcatg gaaaacctgg tagcctatgc 4201 taagaaagtg gaaggggaca tgtacgagtc tgccaacagc agggatgaat attatcactt 4261 attagcagag aaaatctaca agatacaaaa agaactagaa gaaaaacgga ggtcgcgttt 4321 acataaacaa ggcatcttgg ggaaccagcc agccttacca gccccggggg ctcagccccc 4381 tgtgattcca caggcacaac ctgtgagacc tccaaatgga cccctgtccc tgccagtgaa 4441 tcgcatgcaa gtttctcaag ggatgaattc atttaacccc atgtccttgg ggaacgtcca 4501 gttgccacaa gcacccatgg gacctcgtgc agcctcccca atgaaccact ctgtccagat 4561 gaacagcatg ggctcagtgc cagggatggc catttctcct tcccgaatgc ctcagcctcc 4621 gaacatgatg ggtgcacaca ccaacaacat gatggcccag gcgcccgctc agagccagtt 4681 tctgccacag aaccagttcc cgtcatccag cggggcgatg agtgtgggca tggggcagcc 4741 gccagcccaa acaggcgtgt cacagggaca ggtgcctggt gctgctcttc ctaaccctct 4801 caacatgctg gggcctcagg ccagccagct accttgccct ccagtgacac agtcaccact 4861 gcacccaaca ccgcctcctg cttccacggc tgctggcatg ccatctctcc agcacacgac 4921 accacctggg atgactcctc cccagccagc agctcccact cagccatcaa ctcctgtgtc 4981 gtcttccggg cagactccca ccccgactcc tggctcagtg cccagtgcta cccaaaccca 5041 gagcacccct acagtccagg cagcagccca ggcccaggtg accccgcagc ctcaaacccc 5101 agttcagccc ccgtctgtgg ctacccctca gtcatcgcag caacagccga cgcctgtgca 5161 cgcccagcct cctggcacac cgctttccca ggcagcagcc agcattgata acagagtccc 5221 taccccctcc tcggtggcca gcgcagaaac caattcccag cagccaggac ctgacgtacc 5281 tgtgctggaa atgaagacgg agacccaagc agaggacact gagcccgatc ctggtgaatc 5341 caaaggggag cccaggtctg agatgatgga ggaggatttg caaggagctt cccaagttaa 5401 agaagaaaca gacatagcag agcagaaatc agaaccaatg gaagtggatg aaaagaaacc 5461 tgaagtgaaa gtagaagtta aagaggaaga agagagtagc agtaacggca cagcctctca 5521 gtcaacatct ccttcgcagc cgcgcaaaaa aatctttaaa ccagaggagt tacgccaggc 5581 cctcatgcca accctagaag cactgtatcg acaggaccca gagtcattac ctttccggca 5641 gcctgtagat ccccagctcc tcggaattcc agactatttt gacatcgtaa agaatcccat 5701 ggacctctcc accatcaagc ggaagctgga cacagggcaa taccaagagc cctggcagta 5761 cgtggacgac gtctggctca tgttcaacaa tgcctggctc tataatcgca agacatcccg 5821 agtctataag ttttgcagta agcttgcaga ggtctttgag caggaaattg accctgtcat 5881 gcagtccctt ggatattgct gtggacgcaa gtatgagttt tccccacaga ctttgtgctg 5941 ctatgggaag cagctgtgta ccattcctcg cgatgctgcc tactacagct atcagaatag 6001 gtatcatttc tgtgagaagt gtttcacaga gatccagggc gagaatgtga ccctgggtga 6061 cgacccttca cagccccaga cgacaatttc aaaggatcag tttgaaaaga agaaaaatga 6121 taccttagac cccgaacctt tcgttgattg caaggagtgt ggccggaaga tgcatcagat 6181 ttgcgttctg cactatgaca tcatttggcc ttcaggtttt gtgtgcgaca actgcttgaa 6241 gaaaactggc agacctcgaa aagaaaacaa attcagtgct aagaggctgc agaccacaag 6301 actgggaaac cacttggaag accgagtgaa caaatttttg cggcgccaga atcaccctga 6361 agccggggag gtttttgtcc gagtggtggc cagctcagac aagacggtgg aggtcaagcc 6421 cgggatgaag tcacggtttg tggattctgg ggaaatgtct gaatctttcc catatcgaac 6481 caaagctctg tttgcttttg aggaaattga cggcgtggat gtctgctttt ttggaatgca 6541 cgtccaagaa tacggctctg attgcccccc tccaaacacg aggcgtgtgt acatttctta 6601 tctggatagt attcatttct tccggccacg ttgcctccgc acagccgttt accatgagat 6661 ccttattgga tatttagagt atgtgaagaa attagggtat gtgacagggc acatctgggc 6721 ctgtcctcca agtgaaggag atgattacat cttccattgc cacccacctg atcaaaaaat 6781 acccaagcca aaacgactgc aggagtggta caaaaagatg ctggacaagg cgtttgcaga 6841 gcggatcatc catgactaca aggatatttt caaacaagca actgaagaca ggctcaccag 6901 tgccaaggaa ctgccctatt ttgaaggtga tttctggccc aatgtgttag aagagagcat 6961 taaggaacta gaacaagaag aagaggagag gaaaaaggaa gagagcactg cagccagtga 7021 aaccactgag ggcagtcagg gcgacagcaa gaatgccaag aagaagaaca acaagaaaac 7081 caacaagaac aaaagcagca tcagccgcgc caacaagaag aagcccagca tgcccaacgt 7141 gtccaatgac ctgtcccaga agctgtatgc caccatggag aagcacaagg aggtcttctt 7201 cgtgatccac ctgcacgctg ggcctgtcat caacaccctg ccccccatcg tcgaccccga 7261 ccccctgctc agctgtgacc tcatggatgg gcgcgacgcc ttcctcaccc tcgccagaga 7321 caagcactgg gagttctcct ccttgcgccg ctccaagtgg tccacgctct gcatgctggt 7381 ggagctgcac acccagggcc aggaccgctt tgtctacacc tgcaacgagt gcaagcacca 7441 cgtggagacg cgctggcact gcactgtgtg cgaggactac gacctctgca tcaactgcta 7501 taacacgaag agccatgccc ataagatggt gaagtggggg ctgggcctgg atgacgaggg 7561 cagcagccag ggcgagccac agtcaaagag cccccaggag tcacgccggc tgagcatcca 7621 gcgctgcatc cagtcgctgg tgcacgcgtg ccagtgccgc aacgccaact gctcgctgcc 7681 atcctgccag aagatgaagc gggtggtgca gcacaccaag ggctgcaaac gcaagaccaa 7741 cgggggctgc ccggtgtgca agcagctcat cgccctctgc tgctaccacg ccaagcactg 7801 ccaagaaaac aaatgccccg tgcccttctg cctcaacatc aaacacaagc tccgccagca 7861 gcagatccag caccgcctgc agcaggccca gctcatgcgc cggcggatgg ccaccatgaa 7921 cacccgcaac gtgcctcagc agagtctgcc ttctcctacc tcagcaccgc ccgggacccc 7981 cacacagcag cccagcacac cccagacgcc gcagccccct gcccagcccc aaccctcacc 8041 cgtgagcatg tcaccagctg gcttccccag cgtggcccgg actcagcccc ccaccacggt 8101 gtccacaggg aagcctacca gccaggtgcc ggccccccca cccccggccc agccccctcc 8161 tgcagcggtg gaagcggctc ggcagatcga gcgtgaggcc cagcagcagc agcacctgta 8221 ccgggtgaac atcaacaaca gcatgccccc aggacgcacg ggcatgggga ccccggggag 8281 ccagatggcc cccgtgagcc tgaatgtgcc ccgacccaac caggtgagcg ggcccgtcat 8341 gcccagcatg cctcccgggc agtggcagca ggcgcccctt ccccagcagc agcccatgcc 8401 aggcttgccc aggcctgtga tatccatgca ggcccaggcg gccgtggctg ggccccggat 8461 gcccagcgtg cagccaccca ggagcatctc acccagcgct ctgcaagacc tgctgcggac 8521 cctgaagtcg cccagctccc ctcagcagca acagcaggtg ctgaacattc tcaaatcaaa 8581 cccgcagcta atggcagctt tcatcaaaca gcgcacagcc aagtacgtgg ccaatcagcc 8641 cggcatgcag ccccagcctg gcctccagtc ccagcccggc atgcaacccc agcctggcat 8701 gcaccagcag cccagcctgc agaacctgaa tgccatgcag gctggcgtgc cgcggcccgg 8761 tgtgcctcca cagcagcagg cgatgggagg cctgaacccc cagggccagg ccttgaacat 8821 catgaaccca ggacacaacc ccaacatggc gagtatgaat ccacagtacc gagaaatgtt 8881 acggaggcag ctgctgcagc agcagcagca acagcagcag caacaacagc agcaacagca 8941 gcagcagcaa gggagtgccg gcatggctgg gggcatggcg gggcacggcc agttccagca 9001 gcctcaagga cccggaggct acccaccggc catgcagcag cagcagcgca tgcagcagca 9061 tctccccctc cagggcagct ccatgggcca gatggcggct cagatgggac agcttggcca 9121 gatggggcag ccggggctgg gggcagacag cacccccaac atccagcaag ccctgcagca 9181 gcggattctg cagcaacagc agatgaagca gcagattggg tccccaggcc agccgaaccc 9241 catgagcccc cagcaacaca tgctctcagg acagccacag gcctcgcatc tccctggcca 9301 gcagatcgcc acgtccctta gtaaccaggt gcggtctcca gcccctgtcc agtctccacg 9361 gccccagtcc cagcctccac attccagccc gtcaccacgg atacagcccc agccttcgcc 9421 acaccacgtc tcaccccaga ctggttcccc ccaccccgga ctcgcagtca ccatggccag 9481 ctccatagat cagggacact tggggaaccc cgaacagagt gcaatgctcc cccagctgaa 9541 cacccccagc aggagtgcgc tgtccagcga actgtccctg gtcggggaca ccacggggga 9601 cacgctagag aagtttgtgg agggcttgCT CGAGGACTAC AAAGACCATG ACGGTGATTA 9661 TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAGcggccgc aaggatctgc 9721 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 9781 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 9841 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 9901 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 9961 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 10021 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 10081 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 10141 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 10201 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgtcg 10261 acatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg 10321 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg 10381 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag 10441 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca 10501 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt 10561 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg 10621 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag 10681 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt 10741 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg 10801 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg 10861 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg 10921 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg 10981 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc 11041 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg 11101 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg 11161 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg 11221 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa 11281 aggaataggt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 11341 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 11401 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 11461 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 11521 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 11581 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 11641 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 11701 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 11761 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 11821 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 11881 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 11941 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt 12001 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 12061 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 12121 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 12181 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 12241 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 12301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 12361 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc 12421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 12481 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 12541 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt 12601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 12661 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 12721 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 12781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 12841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 12901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 12961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 13021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 13081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 13141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 13201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 13261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 13321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 13381 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 13441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 13501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 13561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 13621 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 13681 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 13741 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 13801 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 13861 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 13921 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 13981 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 14041 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 14101 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 14161 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 14221 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 14281 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 14341 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 14401 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 14461 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 14521 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 14581 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 14641 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 14701 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 14761 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 14821 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 14881 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 14941 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 15001 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 15061 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 15121 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 15181 cgacggccag tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.