pLV3-CMV-GLA-3×FLAG-CopGFP-Pur

pLV3-CMV-GLA-3×FLAG-CopGFP-Puro plasmid

PVT96868

pLV3-CMV-GLA-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-GLA(human)-3×FLAG-CopGFP-Puro
Alias: NM_000169.3;GALA
Gene lenght: 1287bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GLA-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-GLA-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-GLA-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc agctgaggaa cccagaacta 2341 catctgggct gcgcgcttgc gcttcgcttc ctggccctcg tttcctggga catccctggg 2401 gctagagcac tggacaatgg attggcaagg acgcctacca tgggctggct gcactgggag 2461 cgcttcatgt gcaaccttga ctgccaggaa gagccagatt cctgcatcag tgagaagctc 2521 ttcatggaga tggcagagct catggtctca gaaggctgga aggatgcagg ttatgagtac 2581 ctctgcattg atgactgttg gatggctccc caaagagatt cagaaggcag acttcaggca 2641 gaccctcagc gctttcctca tgggattcgc cagctagcta attatgttca cagcaaagga 2701 ctgaagctag ggatttatgc agatgttgga aataaaacct gcgcaggctt ccctgggagt 2761 tttggatact acgacattga tgcccagacc tttgctgact ggggagtaga tctgctaaaa 2821 tttgatggtt gttactgtga cagtttggaa aatttggcag atggttataa gcacatgtcc 2881 ttggccctga ataggactgg cagaagcatt gtgtactcct gtgagtggcc tctttatatg 2941 tggccctttc aaaagcccaa ttatacagaa atccgacagt actgcaatca ctggcgaaat 3001 tttgctgaca ttgatgattc ctggaaaagt ataaagagta tcttggactg gacatctttt 3061 aaccaggaga gaattgttga tgttgctgga ccagggggtt ggaatgaccc agatatgtta 3121 gtgattggca actttggcct cagctggaat cagcaagtaa ctcagatggc cctctgggct 3181 atcatggctg ctcctttatt catgtctaat gacctccgac acatcagccc tcaagccaaa 3241 gctctccttc aggataagga cgtaattgcc atcaatcagg accccttggg caagcaaggg 3301 taccagctta gacagggaga caactttgaa gtgtgggaac gacctctctc aggcttagcc 3361 tgggctgtag ctatgataaa ccggcaggag attggtggac ctcgctctta taccatcgca 3421 gttgcttccc tgggtaaagg agtggcctgt aatcctgcct gcttcatcac acagctcctc 3481 cctgtgaaaa ggaagctagg gttctatgaa tggacttcaa ggttaagaag tcacataaat 3541 cccacaggca ctgttttgct tcagctagaa aatacaatgc agatgtcatt aaaagactta 3601 cttggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 3661 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 3721 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 3781 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 3841 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 3901 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 3961 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 4021 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 4081 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 4141 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 4201 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 4261 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 4321 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 4381 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 4441 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 4501 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 4561 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 4621 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 4681 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 4741 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 4801 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 4861 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 4921 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 4981 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 5041 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 5101 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5161 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5221 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 5281 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 5341 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5401 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5461 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5521 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 5581 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 5641 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 5701 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 5761 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 5821 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 5881 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 5941 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 6001 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 6061 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 6121 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 6181 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 6241 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 6301 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 6361 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 6421 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 6481 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 6541 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 6601 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 6661 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 6721 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 6781 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6841 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6901 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 6961 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 7021 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 7081 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 7141 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 7201 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 7261 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 7321 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 7381 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 7441 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7501 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 7561 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 7621 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 7681 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7741 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 7801 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 7861 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 7921 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7981 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 8041 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 8101 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 8161 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 8221 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 8281 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 8341 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8401 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8461 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 8521 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 8581 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 8641 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 8701 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 8761 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 8821 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 8881 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 8941 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 9001 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 9061 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 9121 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 9181 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 9241 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 9301 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 9361 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 9421 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 9481 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 9541 gtcacgacgt tgtaaaacga cggccagtgc caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.