pLV3-CMV-Hspa8-3×FLAG-mCherry-

pLV3-CMV-Hspa8-3×FLAG-mCherry-Hyg plasmid

PVT96844

pLV3-CMV-Hspa8-3×FLAG-mCherry-Hyg plasmid Information

Plasmid name: pLV3-CMV-Hspa8(mouse)-3×FLAG-mCherry-Hyg
Alias: NM_031165.5;2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;Hspa10;
Gene lenght: 1938bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Hspa8-3×FLAG-mCherry-Hyg plasmid Description

pLV3-CMV-Hspa8-3×FLAG-mCherry-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Hspa8-3×FLAG-mCherry-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcA AGCTTggatc cGCCACCatg tctaagggac ctgcagttgg cattgatctc 2341 ggcaccacct actcctgtgt gggtgtcttc cagcatggaa aggtggaaat tattgccaat 2401 gaccagggta accgcaccac gccaagctat gttgctttca cggacacaga gagattaatt 2461 ggggatgcgg ccaagaatca ggttgcaatg aaccccacca acacagtttt tgatgccaaa 2521 cgtctgatcg ggcgtaggtt tgatgatgct gttgttcagt ctgatatgaa gcactggccc 2581 ttcatggtgg tgaatgatgc aggcaggccc aaggtccaag tggaatacaa aggggagaca 2641 aaaagtttct acccagagga agtgtcctcc atggttctga caaagatgaa ggaaattgca 2701 gaagcgtacc tcggaaagac cgttaccaac gctgtggtca cagtgcccgc ttacttcaat 2761 gactctcagc gacaggcaac aaaagatgct ggaactattg ctggcctcaa tgtacttcga 2821 atcatcaatg aaccaactgc tgctgctatt gcttacggct tagataagaa ggtcggagct 2881 gaaaggaatg tgctcatttt tgacttggga ggtggcactt ttgatgtgtc aatcctcact 2941 attgaggatg gaatttttga ggtcaaatca acagctggag acacccactt aggtggagaa 3001 gattttgaca accgaatggt caatcatttc attgctgagt tcaagcgaaa gcacaagaaa 3061 gacatcagtg agaacaagag agctgtccgc cgtctccgca cggcctgcga gcgggccaag 3121 cgcaccctct cctccagcac ccaggccagt attgagattg attctctcta tgagggaatt 3181 gacttctata cctccattac ccgggctcga tttgaggagt tgaatgctga cctgttccgt 3241 ggcacactgg accctgtaga gaaggccctt cgagatgcca agctggacaa gtcacagatc 3301 catgatattg tcttggtggg tggttctacc agaatcccca agattcagaa acttctgcaa 3361 gacttcttca atggaaaaga gctgaacaag agcattaacc ccgatgaagc tgttgcctat 3421 ggtgcagctg tccaggcagc cattctatct ggagacaagt ctgagaacgt tcaggatttg 3481 ctgctcttgg atgtcactcc tctttccctt ggtattgaaa ctgctggcgg agtcatgact 3541 gtcctcatca agcgcaatac caccatcccc accaagcaga cacagacttt caccacctac 3601 tctgacaacc agcctggtgt actcattcag gtgtatgaag gtgaaagggc catgaccaag 3661 gacaacaacc tgcttggaaa gttcgagctc acaggcatcc ctccagcacc ccgtggggtc 3721 cctcagattg aggttacttt tgacatcgat gccaatggca tcctcaatgt ttctgctgta 3781 gataagagca caggaaagga gaacaagatc accatcacca atgacaaggg ccgcttgagt 3841 aaggaagata ttgagcgcat ggtccaagaa gctgagaagt acaaggctga ggatgagaag 3901 cagagagata aggtttcctc caagaactca ctggagtcct atgccttcaa catgaaagca 3961 actgtggaag atgagaaact tcaaggcaag atcaatgatg aggacaaaca gaagattctt 4021 gacaagtgca atgaaatcat cagctggctg gataagaacc agactgcaga gaaggaagaa 4081 tttgagcatc agcagaaaga actggagaaa gtctgcaacc ctatcattac caagctgtac 4141 cagagtgcag gtggcatgcc tgggggaatg cctggtggct tcccaggtgg aggagctccc 4201 ccatctggtg gtgcttcttc aggccccacc attgaagagg tggatCTCGA GatgGACTAC 4261 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 4321 TAGgcggccg cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc 4381 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag 4441 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 4501 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 4561 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg 4621 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg 4681 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct 4741 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac 4801 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa 4861 gctgtgaccg gcgcctacgc tagacgccac cATGGTGAGC AAGGGCGAGG AGGATAACAT 4921 GGCCATCATC AAGGAGTTCA TGCGCTTCAA GGTGCACATG GAGGGCTCCG TGAACGGCCA 4981 CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA 5041 GCTGAAGGTG ACCAAGGGTG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT 5101 CATGTACGGC TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT 5161 GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT 5221 GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG 5281 CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC 5341 CTCCTCCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGCAGAGGCT 5401 GAAGCTGAAG GACGGCGGCC ACTACGACGC TGAGGTCAAG ACCACCTACA AGGCCAAGAA 5461 GCCCGTGCAG CTGCCCGGCG CCTACAACGT CAACATCAAG TTGGACATCA CCTCCCACAA 5521 CGAGGACTAC ACCATCGTGG AACAGTACGA ACGCGCCGAG GGCCGCCACT CCACCGGCGG 5581 CATGGACGAG CTGTACAAGG TCGAGGGAAG CGGAGCTACT AACTTCAGCC TGCTGAAGCA 5641 GGCTGGAGAC GTGGAGGAGA ACCCTGGACC TGTCGACatg aaaaagcctg aactcaccgc 5701 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct 5761 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct 5821 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc 5881 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac 5941 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact 6001 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag 6061 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg 6121 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga 6181 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg 6241 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa 6301 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga 6361 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta 6421 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg 6481 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg 6541 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca 6601 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag 6661 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagTCTAGAa atcaacctct 6721 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 6781 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 6841 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 6901 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 6961 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 7021 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 7081 caattccgtg gtgttgtcgg ggaaATCATC GTCCTTTCCT tggctgctcg cctgtgttgc 7141 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 7201 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc 7261 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga 7321 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc 7381 taattcactc ccaacgaaga taagatctgc tttttgcttg tactgggtct ctctggttag 7441 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 7501 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 7561 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc 7621 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact 7681 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 7741 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 7801 atgtctggct ctagctatcc cgcccctaac tccgccATCC CGCCCTAACT CCGCcagttc 7861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 7921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg 7981 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 8041 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 8101 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 8161 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 8221 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 8281 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 8341 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 8401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 8461 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 8521 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8581 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 8641 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8701 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8761 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8821 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 8881 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 8941 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 9001 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 9061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 9121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 9181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 9241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 9301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 9361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 9421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 9481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 9541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 9601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 9661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 9721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 9781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 9841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 9901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 9961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 10021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 10081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 10141 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 10201 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 10261 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 10321 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 10381 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 10441 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 10501 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 10561 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 10621 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.