pLV2-EF1a-Myc-Upf1-IRES-Puro p

pLV2-EF1a-Myc-Upf1-IRES-Puro plasmid

PVT96835

pLV2-EF1a-Myc-Upf1-IRES-Puro plasmid Information

Plasmid name: pLV2-EF1a-Myc-Upf1(mouse)-IRES-Puro
Alias: NM_001122829.3;B430202H16Rik; NORF1; PNORF-1; Rent1;Upflp
Gene lenght: 3375bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-Myc-Upf1-IRES-Puro plasmid Description

pLV2-EF1a-Myc-Upf1-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-EF1a-Myc-Upf1-IRES-Puro plasmid Sequence

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC 1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATGAGGCC CTTTCGTCTT CACTCGAGGT GCCCGTCAGT 2221 GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA 2281 CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC 2341 GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC 2401 TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC 2461 CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT 2521 ACGTGATTCT TGATCCCGAG CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG 2581 CTTAAGGAGC CCCTTCGCCT CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC 2641 GCGTGCGAAT CTGGTGGCAC CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA 2701 TTTAAAATTT TTGATGACCT GCTGCGACGC TTTTTTTCTG GCAAGATAGT CTTGTAAATG 2761 CGGGCCAAGA TCTGCACACT GGTATTTCGG TTTTTGGGGC CGCGGGCGGC GACGGGGCCC 2821 GTGCGTCCCA GCGCACATGT TCGGCGAGGC GGGGCCTGCG AGCGCGGCCA CCGAGAATCG 2881 GACGGGGGTA GTCTCAAGCT GGCCGGCCTG CTCTGGTGCC TGGCCTCGCG CCGCCGTGTA 2941 TCGCCCCGCC CTGGGCGGCA AGGCTGGCCC GGTCGGCACC AGTTGCGTGA GCGGAAAGAT 3001 GGCCGCTTCC CGGCCCTGCT GCAGGGAGCT CAAAATGGAG GACGCGGCGC TCGGGAGAGC 3061 GGGCGGGTGA GTCACCCACA CAAAGGAAAA GGGCCTTTCC GTCCTCAGCC GTCGCTTCAT 3121 GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG AGCTTTTGGA 3181 GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC CACACTGAGT 3241 GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG GAATTTGCCC 3301 TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC 3361 TTCCATTTCA GGTGTCGTGA GGATCCGCCA CCatgGAACA AAAACTCATC TCAGAAGAGG 3421 ATCTGatgag tgtggaggcg tacggcccca gctcgcaaac actcaccttc ttggacactg 3481 aggaggccga gctgctcggc gccgacaccc agggctccga gttcgaattc accgacttca 3541 cccttcccag ccagacgcag acgccccccg gcggccccgg cggcgcggga ggcccgggcg 3601 gagcgggcgc aggcggcgcg gccggccagc tcgacgcaca agttggacca gagggcatct 3661 tgcaaaatgg ggctgtggat gacagtgtgg ccaagaccag ccagctgcta gctgagctga 3721 acttcgagga agatgaagag gacacatact acactaagga cctcccagtc cacgcctgca 3781 gttactgtgg aatccatgat cctgcctgcg tggtttactg taataccagc aagaagtggt 3841 tctgcaatgg ccgaggaaat acttctggca gccacattgt gaatcacctc gtgagggcaa 3901 aatgcaagga agtgacgctg cacaaggacg ggcctctggg cgagaccgtg ctggagtgct 3961 acaactgtgg ctgccgcaac gtcttcctgc tgggcttcat ccctgcgaag gccgactctg 4021 tggtggtgct gttgtgcagg cagccctgtg ccagccagag cagcctgaag gacatcaact 4081 gggacagctc acagtggcag cccctaatcc aggaccggtg ctttctgtca tggctggtca 4141 agattccctc tgagcaggag cagctgcgag cacggcagat cacggcacag cagatcaaca 4201 agctggaaga gctctggaag gaaaatcctt cagccactct ggaggacctg gagaagccag 4261 gcgtagacga ggagccacag cacgtgctcc tgcgttacga ggatgcttac cagtaccaga 4321 acatcttcgg gccactggtc aagctggagg ctgactatga caagaagttg aaggagtcac 4381 agactcaaga taacatcacg gtcaggtggg acctgggcct taacaagaag agaatcgcct 4441 tcttcacttt gcccaagact gactctggta atgaggattt agtcataatt tggttaagag 4501 acatgcggct catgcagggt gatgagatct gtctgcggta caaaggggat ctggcgcccc 4561 tgtggaaggg gattggccac gtcatcaagg ttcctgataa ttatggtgat gagattgcta 4621 ttgagctccg cagcagcgtg ggtgcccctg tggaagtgac ccacaacttc caagtggatt 4681 ttgtgtggaa gtcaacctct tttgatagga tgcagagtgc actgaagacc ttcgctgtgg 4741 acgagacctc tgtgtcaggg tatatttacc acaagctgct gggccacgag gtggaggatg 4801 tggtcatcaa gtgccagctg ccaaagcgct tcacagctca ggggctccct gacctcaacc 4861 actctcaggt gtatgctgtg aagaccgtgc tgcagagacc actcagcctc atccagggcc 4921 ctccaggcac aggcaagact gtgacatcag ccactattgt ctaccacctt gctcggcagg 4981 gcaatgggcc tgtactggtt tgtgctccaa gtaacatcgc tgtggaccag ctcacagaga 5041 agatccacca gacaggactg aaggtcgtac gcctctgtgc caagagccgt gaggccattg 5101 actccccagt gtccttcctg gctttgcaca accagatcag gaacatggac agcatgcctg 5161 agctgcagaa gctgcagcag ctaaaggatg agacaggcga gctgtcatct gcagatgaga 5221 agcggtaccg ggcgcttaag cgcacagctg agagagaact tctcatgaat gcagatgtca 5281 tatgctgcac atgtgtgggt gctggtgacc cgaggctggc caagatgcag ttccgttcca 5341 tcctcattga cgagagcacc caggccactg agcctgagtg catggtgcct gtagtccttg 5401 gggccaagca gctaatcctc gtcggtgacc actgccagct gggcccagtg gtgatgtgca 5461 agaaggcagc caaggccgga ctgtcacaat cgctcttcga gcgcttggtg gtgctgggca 5521 tccggcccat ccgcctgcag gtgcaatacc gcatgcaccc tgcactcagc gcctttccgt 5581 ccaacatctt ctacgagggc tcattgcaga atggcgtcac tgcagcggat cgtgtcaaaa 5641 aaggctttga cttccagtgg ccacaacctg acaagcctat gttcttctac gtgacgcagg 5701 gccaggagga gattgccagc tctggcacat cctacctcaa caggacggag gcagccaatg 5761 tggagaagat aactacgaag ctgttgaagg caggtgcaaa gcctgaccag atcggcatca 5821 tcacccccta cgagggccag cgctcttact tggtgcagta catgcagttc agcggctccc 5881 tgcacacaaa gctctaccag gaagtggaaa ttgccagtgt ggacgccttc cagggccggg 5941 agaaggactt catcattctg tcctgcgtgc gcgccaatga acatcagggc attgggttcc 6001 taaacgaccc ccggcgtctg aatgtggctc tcaccagagc aagatatggc gtgatcattg 6061 tgggtaaccc aaaggccctg tcgaagcagc ccctgtggaa tcacctgctg agctactaca 6121 aggaacagaa ggcgctagtg gaagggccgc tcaacaacct acgtgagagc ctcatgcagt 6181 tcagcaagcc tcgcaaactt gtcaacactg tcaacccggg tgcccgcttc atgactactg 6241 ccatgtacga tgcccgtgag gccatcatcc ccgggtctgt ctatgaccgc agcagccagg 6301 gccggccctc gaacatgtac ttccagaccc atgaccagat tagtatgatc agcgcaggcc 6361 ccagccacgt ggctgccatg aacatcccta ttcccttcaa cttggtcatg cctcccatgc 6421 cgccacctgg ctacttcgga caggccaacg ggccggcagc tggtcggggc accccaaaaa 6481 ccaagactgg ccgtgggggc cgccagaaga accgctttgg gcttcctggg cccagccaga 6541 ccacccttcc caacagccag gccagccagg acgtggcctc ccagcccttt tcacagggtg 6601 ccctcacaca gggttacgtg tccatgagcc agccctctca gatgagccag cctggcctct 6661 cccagccaga actgtcccag gacagctacc tcggtgatga gtttaaatca cagattgacg 6721 tggcactctc acaagactcc acataccagg gagagcgggc ataccagcac ggcggggtca 6781 ccgggctgtc ccagtactag ACGCGTCTCG AGGGCGGCCG CTCTAGAGTC GACGGGCCGC 6841 GGTAACAATT GTTAACTAAC TTAAGCTAGC AACGGTTTCC CTCTAGCGGG ATCAATTCCG 6901 CCCCCCCCCC CTAACGTTAC TGGCCGAAGC CGCTTGGAAT AAGGCCGGTG TGCGTTTGTC 6961 TATATGTTAT TTTCCACCAT ATTGCCGTCT TTTGGCAATG TGAGGGCCCG GAAACCTGGC 7021 CCTGTCTTCT TGACGAGCAT TCCTAGGGGT CTTTCCCCTC TCGCCAAAGG AATGCAAGGT 7081 CTGTTGAATG TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT CTTGAAGACA AACAACGTCT 7141 GTAGCGACCC TTTGCAGGCA GCGGAACCCC CCACCTGGCG ACAGGTGCCT CTGCGGCCAA 7201 AAGCCACGTG TATAAGATAC ACCTGCAAAG GCGGCACAAC CCCAGTGCCA CGTTGTGAGT 7261 TGGATAGTTG TGGAAAGAGT CAAATGGCTC TCCTCAAGCG TATTCAACAA GGGGCTGAAG 7321 GATGCCCAGA AGGTACCCCA TTGTATGGGA TCTGATCTGG GGCCTCGGTG CACATGCTTT 7381 ACATGTGTTT AGTCGAGGTT AAAAAAACGT CTAGGCCCCC CGAACCACGG GGACGTGGTT 7441 TTCCTTTGAA AAACACGATA ATACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC 7501 CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC 7561 CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT 7621 CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC 7681 GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG 7741 CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT 7801 CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC 7861 CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA 7921 GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA 7981 GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG 8041 CATGACCCGC AAGCCCGGTG CCTGACCCGG GCGGGGCGCG TCTGGAACAA TCAACCTCTG 8101 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 8161 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 8221 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 8281 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 8341 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 8401 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 8461 AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC 8521 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 8581 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 8641 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GAATTAATTC TGCAGTCGAG 8701 ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG 8761 CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT 8821 TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGAGGG 8881 GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC 8941 ACACACAAGG CTACTTCCCT GATTAGCAGA ACTACACACC AGGGCCAGGG GTCAGATATC 9001 CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA GCCAGATAAG GTAGAAGAGG 9061 CCAATAAAGG AGAGAACACC AGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC 9121 CGGAGAGAGA AGTGTTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACGTGGCCC 9181 GAGAGCTGCA TCCGGAGTAC TTCAAGAACT GCTGATATCG AGCTTGCTAC AAGGGACTTT 9241 CCGCTGGGGA CTTTCCAGGG AGGCGTGGCC TGGGCGGGAC TGGGGAGTGG CGAGCCCTCA 9301 GATCCTGCAT ATAAGCAGCT GCTTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC 9361 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG 9421 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC 9481 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA GTAGTTCATG TCATCTTATT 9541 ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA GTGAGAGGCC TTGACATTGC 9601 TAGCGTTTAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 9661 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 9721 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 9781 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 9841 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 9901 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 9961 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 10021 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 10081 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 10141 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 10201 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 10261 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 10321 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 10381 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 10441 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT 10501 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 10561 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 10621 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 10681 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 10741 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 10801 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 10861 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 10921 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 10981 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 11041 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 11101 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 11161 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 11221 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 11281 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 11341 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 11401 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 11461 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 11521 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 11581 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 11641 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 11701 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 11761 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCAA 11821 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 11881 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 11941 TCATGTCTGG ATCAACTGGA TAACTCAAGC TAACCAAAAT CATCCCAAAC TTCCCACCCC 12001 ATACCCTATT ACCACTGCCA ATTACCTGTG GTTTCATTTA CTCTAAACCT GTGATTCCTC 12061 TGAATTATTT TCATTTTAAA GAAATTGTAT TTGTTAAATA TGTACTACAA ACTTAGTAGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.