pCMV-COL7A1-3×FLAG-Neo plasmid

pCMV-COL7A1-3×FLAG-Neo plasmid

PVT96795

pCMV-COL7A1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-COL7A1(human)-3×FLAG-Neo
Alias: NM_000094.3;EBD1; EBDCT; EBR1; NDNC8
Gene lenght: 8832bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-COL7A1-3×FLAG-Neo plasmid Description

pCMV-COL7A1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-COL7A1-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCGCC 961 ACCatgacgc tgcggcttct ggtggccgcg ctctgcgccg ggatcctggc agaggcgccc 1021 cgagtgcgag cccagcacag ggagagagtg acctgcacgc gcctttacgc cgctgacatt 1081 gtgttcttac tggatggctc ctcatccatt ggccgcagca atttccgcga ggtccgcagc 1141 tttctcgaag ggctggtgct gcctttctct ggagcagcca gtgcacaggg tgtgcgcttt 1201 gccacagtgc agtacagcga tgacccacgg acagagttcg gcctggatgc acttggctct 1261 gggggtgatg tgatccgcgc catccgtgag cttagctaca aggggggcaa cactcgcaca 1321 ggggctgcaa ttctccatgt ggctgaccat gtcttcctgc cccagctggc ccgacctggt 1381 gtccccaagg tctgcatcct gatcacagac gggaagtccc aggacctggt ggacacagct 1441 gcccaaaggc tgaaggggca gggggtcaag ctatttgctg tggggatcaa gaatgctgac 1501 cctgaggagc tgaagcgagt tgcctcacag cccaccagtg acttcttctt cttcgtcaat 1561 gacttcagca tcttgaggac actactgccc ctcgtttccc ggagagtgtg cacgactgct 1621 ggtggcgtgc ctgtgacccg acctccggat gactcgacct ctgctccacg agacctggtg 1681 ctgtctgagc caagcagcca atccttgaga gtacagtgga cagcggccag tggccctgtg 1741 actggctaca aggtccagta cactcctctg acggggctgg gacagccact gccgagtgag 1801 cggcaggagg tgaacgtccc agctggtgag accagtgtgc ggctgcgggg tctccggcca 1861 ctgaccgagt accaagtgac tgtgattgcc ctctacgcca acagcatcgg ggaggctgtg 1921 agcgggacag ctcggaccac tgccctagaa gggccggaac tgaccatcca gaataccaca 1981 gcccacagcc tcctggtggc ctggcggagt gtgccaggtg ccactggcta ccgtgtgaca 2041 tggcgggtcc tcagtggtgg gcccacacag cagcaggagc tgggccctgg gcagggttca 2101 gtgttgctgc gtgacttgga gcctggcacg gactatgagg tgaccgtgag caccctattt 2161 ggccgcagtg tggggcccgc cacttccctg atggctcgca ctgacgcttc tgttgagcag 2221 accctgcgcc cggtcatcct gggccccaca tccatcctcc tttcctggaa cttggtgcct 2281 gaggcccgtg gctaccggtt ggaatggcgg cgtgagactg gcttggagcc accgcagaag 2341 gtggtactgc cctctgatgt gacccgctac cagttggatg ggctgcagcc gggcactgag 2401 taccgcctca cactctacac tctgctggag ggccacgagg tggccacccc tgcaaccgtg 2461 gttcccactg gaccagagct gcctgtgagc cctgtaacag acctgcaagc caccgagctg 2521 cccgggcagc gggtgcgagt gtcctggagc ccagtccctg gtgccaccca gtaccgcatc 2581 attgtgcgca gcacccaggg ggttgagcgg accctggtgc ttcctgggag tcagacagca 2641 ttcgacttgg atgacgttca ggctgggctt agctacactg tgcgggtgtc tgctcgagtg 2701 ggtccccgtg agggcagtgc cagtgtcctc actgtccgcc gggagccgga aactccactt 2761 gctgttccag ggctgcgggt tgtggtgtca gatgcaacgc gagtgagggt ggcctgggga 2821 cccgtccctg gagccagtgg atttcggatt agctggagca caggcagtgg tccggagtcc 2881 agccagacac tgcccccaga ctctactgcc acagacatca cagggctgca gcctggaacc 2941 acctaccagg tggctgtgtc ggtactgcga ggcagagagg agggccctgc tgcagtcatc 3001 gtggctcgaa cggacccact gggcccagtg aggacggtcc atgtgactca ggccagcagc 3061 tcatctgtca ccattacctg gaccagggtt cctggcgcca caggatacag ggtttcctgg 3121 cactcagccc acggcccaga gaaatcccag ttggtttctg gggaggccac ggtggctgag 3181 ctggatggac tggagccaga tactgagtat acggtgcatg tgagggccca tgtggctggc 3241 gtggatgggc cccctgcctc tgtggttgtg aggactgccc ctgagcctgt gggtcgtgtg 3301 tcgaggctgc agatcctcaa tgcttccagc gacgttctac ggatcacctg ggtaggggtc 3361 actggagcca cagcttacag actggcctgg ggccggagtg aaggcggccc catgaggcac 3421 cagatactcc caggaaacac agactctgca gagatccggg gtctcgaagg tggagtcagc 3481 tactcagtgc gagtgactgc acttgtcggg gaccgcgagg gcacacctgt ctccattgtt 3541 gtcactacgc cgcctgaggc tccgccagcc ctggggacgc ttcacgtggt gcagcgcggg 3601 gagcactcgc tgaggctgcg ctgggagccg gtgcccagag cgcagggctt ccttctgcac 3661 tggcaacctg agggtggcca ggaacagtcc cgggtcctgg ggcccgagct cagcagctat 3721 cacctggacg ggctggagcc agcgacacag taccgcgtga ggctgagtgt cctagggcca 3781 gctggagaag ggccctctgc agaggtgact gcgcgcactg agtcacctcg tgttccaagc 3841 attgaactac gtgtggtgga cacctcgatc gactcggtga ctttggcctg gactccagtg 3901 tccagggcat ccagctacat cctatcctgg cggccactca gaggccctgg ccaggaagtg 3961 cctgggtccc cgcagacact tccagggatc tcaagctccc agcgggtgac agggctagag 4021 cctggcgtct cttacatctt ctccctgacg cctgtcctgg atggtgtgcg gggtcctgag 4081 gcatctgtca cacagacgcc agtgtgcccc cgtggcctgg cggatgtggt gttcctacca 4141 catgccactc aagacaatgc tcaccgtgcg gaggctacga ggagggtcct ggagcgtctg 4201 gtgttggcac ttgggcctct tgggccacag gcagttcagg ttggcctgct gtcttacagt 4261 catcggccct ccccactgtt cccactgaat ggctcccatg accttggcat tatcttgcaa 4321 aggatccgtg acatgcccta catggaccca agtgggaaca acctgggcac agccgtggtc 4381 acagctcaca gatacatgtt ggcaccagat gctcctgggc gccgccagca cgtaccaggg 4441 gtgatggttc tgctagtgga tgaacccttg agaggtgaca tattcagccc catccgtgag 4501 gcccaggctt ctgggcttaa tgtggtgatg ttgggaatgg ctggagcgga cccagagcag 4561 ctgcgtcgct tggcgccggg tatggactct gtccagacct tcttcgccgt ggatgatggg 4621 ccaagcctgg accaggcagt cagtggtctg gccacagccc tgtgtcaggc atccttcact 4681 actcagcccc ggccagagcc ctgcccagtg tattgtccaa agggccagaa gggggaacct 4741 ggagagatgg gcctgagagg acaagttggg cctcctggcg accctggcct cccgggcagg 4801 accggtgctc ccggccccca ggggccccct ggaagtgcca ctgccaaggg cgagaggggc 4861 ttccctggag cagatgggcg tccaggcagc cctggccgcg ccgggaatcc tgggacccct 4921 ggagcccctg gcctaaaggg ctctccaggg ttgcctggcc ctcgtgggga cccgggagag 4981 cgaggacctc gaggcccaaa gggggagccg ggggctcccg gacaagtcat cggaggtgaa 5041 ggacctgggc ttcctgggcg gaaaggggac cctggaccat cgggcccccc tggacctcgt 5101 ggaccactgg gggacccagg accccgtggc cccccagggc ttcctggaac agccatgaag 5161 ggtgacaaag gcgatcgtgg ggagcggggt ccccctggac caggtgaagg tggcattgct 5221 cctggggagc ctgggctgcc gggtcttccc ggaagccctg gaccccaagg ccccgttggc 5281 ccccctggaa agaaaggaga aaaaggtgac tctgaggatg gagctccagg cctcccagga 5341 caacctgggt ctccgggtga gcagggccca cggggacctc ctggagctat tggccccaaa 5401 ggtgaccggg gctttccagg gcccctgggt gaggctggag agaagggcga acgtggaccc 5461 ccaggcccag cgggatcccg ggggctgcca ggggttgctg gacgtcctgg agccaagggt 5521 cctgaagggc caccaggacc cactggccgc caaggagaga agggggagcc tggtcgccct 5581 ggggaccctg cagtggtggg acctgctgtt gctggaccca aaggagaaaa gggagatgtg 5641 gggcccgctg ggcccagagg agctaccgga gtccaagggg aacggggccc acccggcttg 5701 gttcttcctg gagaccctgg ccccaaggga gaccctggag accggggtcc cattggcctt 5761 actggcagag caggaccccc aggtgactca gggcctcctg gagagaaggg agaccctggg 5821 cggcctggcc ccccaggacc tgttggcccc cgaggacgag atggtgaagt tggagagaaa 5881 ggtgacgagg gtcctccggg tgacccgggt ttgcctggaa aagcaggcga gcgtggcctt 5941 cggggggcac ctggagttcg ggggcctgtg ggtgaaaagg gagaccaggg agatcctgga 6001 gaggatggac gaaatggcag ccctggatca tctggaccca agggtgaccg tggggagccg 6061 ggtcccccag gacccccggg acggctggta gacacaggac ctggagccag agagaaggga 6121 gagcctgggg accgcggaca agagggtcct cgagggccca agggtgatcc tggcctccct 6181 ggagcccctg gggaaagggg cattgaaggg tttcggggac ccccaggccc acagggggac 6241 ccaggtgtcc gaggcccagc aggagaaaag ggtgaccggg gtccccctgg gctggatggc 6301 cggagcggac tggatgggaa accaggagcc gctgggccct ctgggccgaa tggtgctgca 6361 ggcaaagctg gggacccagg gagagacggg cttccaggcc tccgtggaga acagggcctc 6421 cctggcccct ctggtccccc tggattaccg ggaaagccag gcgaggatgg caaacctggc 6481 ctgaatggaa aaaacggaga acctggggac cctggagaag acgggaggaa gggagagaaa 6541 ggagattcag gcgcctctgg gagagaaggt cgtgatggcc ccaagggtga gcgtggagct 6601 cctggtatcc ttggacccca ggggcctcca ggcctcccag ggccagtggg ccctcctggc 6661 cagggttttc ctggtgtccc aggaggcacg ggccccaagg gtgaccgtgg ggagactgga 6721 tccaaagggg agcagggcct ccctggagag cgtggcctgc gaggagagcc tggaagtgtg 6781 ccgaatgtgg atcggttgct ggaaactgct ggcatcaagg catctgccct gcgggagatc 6841 gtggagacct gggatgagag ctctggtagc ttcctgcctg tgcccgaacg gcgtcgaggc 6901 cccaaggggg actcaggcga acagggcccc ccaggcaagg agggccccat cggctttcct 6961 ggagaacgcg ggctgaaggg cgaccgtgga gaccctggcc ctcaggggcc acctggtctg 7021 gcccttgggg agaggggccc ccccgggcct tccggccttg ccggggagcc tggaaagcct 7081 ggtattcccg ggctcccagg cagggctggg ggtgtgggag aggcaggaag gccaggagag 7141 aggggagaac ggggagagaa aggagaacgt ggagaacagg gcagagatgg ccctcctgga 7201 ctccctggaa cccctgggcc ccccggaccc cctggcccca aggtgtctgt ggatgagcca 7261 ggtcctggac tctctggaga acagggaccc cctggactca agggtgctaa gggggagccg 7321 ggcagcaatg gtgaccaagg tcccaaagga gacaggggtg tgccaggcat caaaggagac 7381 cggggagagc ctggaccgag gggtcaggac ggcaacccgg gtctaccagg agagcgtggt 7441 atggctgggc ctgaagggaa gccgggtctg cagggtccaa gaggcccccc tggcccagtg 7501 ggtggtcatg gagaccctgg accacctggt gccccgggtc ttgctggccc tgcaggaccc 7561 caaggacctt ctggcctgaa gggggagcct ggagagacag gacctccagg acggggcctg 7621 actggaccta ctggagctgt gggacttcct ggaccccccg gcccttcagg ccttgtgggt 7681 ccacaggggt ctccaggttt gcctggacaa gtgggggaga cagggaagcc gggagcccca 7741 ggtcgagatg gtgccagtgg aaaagatgga gacagaggga gccctggtgt gccagggtca 7801 ccaggtctgc ctggccctgt cggacctaaa ggagaacctg gccccacggg ggcccctgga 7861 caggctgtgg tcgggctccc tggagcaaag ggagagaagg gagcccctgg aggccttgct 7921 ggagacctgg tgggtgagcc gggagccaaa ggtgaccgag gactgccagg gccgcgaggc 7981 gagaagggtg aagctggccg tgcaggggag cccggagacc ctggggaaga tggtcagaaa 8041 ggggctccag gacccaaagg tttcaagggt gacccaggag tcggggtccc gggctcccct 8101 gggcctcctg gccctccagg tgtgaaggga gatctgggcc tccctggcct gcccggtgct 8161 cctggtgttg ttgggttccc gggtcagaca ggccctcgag gagagatggg tcagccaggc 8221 cctagtggag agcggggtct ggcaggcccc ccagggagag aaggaatccc aggacccctg 8281 gggccacctg gaccaccggg gtcagtggga ccacctgggg cctctggact caaaggagac 8341 aagggagacc ctggagtagg gctgcctggg ccccgaggcg agcgtgggga gccaggcatc 8401 cggggtgaag atggccgccc cggccaggag ggaccccgag gactcacggg gccccctggc 8461 agcaggggag agcgtgggga gaagggtgat gttgggagtg caggactaaa gggtgacaag 8521 ggagactcag ctgtgatcct ggggcctcca ggcccacggg gtgccaaggg ggacatgggt 8581 gaacgagggc ctcggggctt ggatggtgac aaaggacctc ggggagacaa tggggaccct 8641 ggtgacaagg gcagcaaggg agagcctggt gacaagggct cagccgggtt gccaggactg 8701 cgtggactcc tgggacccca gggtcaacct ggtgcagcag ggatccctgg tgacccggga 8761 tccccaggaa aggatggagt gcctggtatc cgaggagaaa aaggagatgt tggcttcatg 8821 ggtccccggg gcctcaaggg tgaacgggga gtgaagggag cctgtggcct tgatggagag 8881 aagggagaca agggagaagc tggtccccca ggccgccccg ggctggcagg acacaaagga 8941 gagatggggg agcctggtgt gccgggccag tcgggggccc ctggcaagga gggcctgatc 9001 ggtcccaagg gtgaccgagg ctttgacggg cagccaggcc ccaagggtga ccagggcgag 9061 aaaggggagc ggggaacccc aggaattggg ggcttcccag gccccagtgg aaatgatggc 9121 tctgctggtc ccccagggcc acctggcagt gttggtccca gaggccccga aggacttcag 9181 ggccagaagg gtgagcgagg tccccccgga gagagagtgg tgggggctcc tggggtccct 9241 ggagctcctg gcgagagagg ggagcagggg cggccagggc ctgccggtcc tcgaggcgag 9301 aagggagaag ctgcactgac ggaggatgac atccggggct ttgtgcgcca agagatgagt 9361 cagcactgtg cctgccaggg ccagttcatc gcatctggat cacgacccct ccctagttat 9421 gctgcagaca ctgccggctc ccagctccat gctgtgcctg tgctccgcgt ctctcatgca 9481 gaggaggaag agcgggtacc ccctgaggat gatgagtact ctgaatactc cgagtattct 9541 gtggaggagt accaggaccc tgaagctcct tgggatagtg atgacccctg ttccctgcca 9601 ctggatgagg gctcctgcac tgcctacacc ctgcgctggt accatcgggc tgtgacaggc 9661 agcacagagg cctgtcaccc ttttgtctat ggtggctgtg gagggaatgc caaccgtttt 9721 gggacccgtg aggcctgcga gcgccgctgc ccaccccggg tggtccagag ccaggggaca 9781 ggtactgccc aggacCTCGA GGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 9841 ATCGACTACA AGGATGACGA TGACAAGTAG TGAGGGCCCG TTTAAACCCG CTGATCAGCC 9901 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG 9961 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT 10021 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG 10081 GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG 10141 GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC 10201 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 10261 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 10321 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 10381 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 10441 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 10501 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 10561 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT 10621 GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 10681 ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 10741 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC 10801 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 10861 TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 10921 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT 10981 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG 11041 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG 11101 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA 11161 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC 11221 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG 11281 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG 11341 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA 11401 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG 11461 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC 11521 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG 11581 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG 11641 GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG 11701 AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG 11761 ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG 11821 GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC 11881 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 11941 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 12001 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 12061 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC 12121 GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 12181 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 12241 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 12301 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 12361 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 12421 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 12481 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 12541 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 12601 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 12661 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 12721 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 12781 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 12841 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 12901 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 12961 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 13021 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 13081 TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 13141 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 13201 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 13261 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 13321 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 13381 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 13441 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 13501 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 13561 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 13621 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 13681 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 13741 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 13801 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 13861 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 13921 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 13981 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 14041 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 14101 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 14161 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 14221 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 14281 TCCCCGAAAA GTGCCACCTG ACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.