pLV3-CMV-TNC-3×FLAG-CopGFP-Pur

pLV3-CMV-TNC-3×FLAG-CopGFP-Puro plasmid

PVT96726

pLV3-CMV-TNC-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TNC(human)-3×FLAG-CopGFP-Puro
Alias: NM_001410991.1;150-225; DFNA56; GMEM; GP; HXB; JI; TN;TN-C
Gene lenght: 6057bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TNC-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TNC-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TNC-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gggccatgac tcagctgttg 2341 gcaggtgtct ttcttgcttt ccttgccctc gctaccgaag gtggggtcct caagaaagtc 2401 atccggcaca agcgacagag tggggtgaac gccaccctgc cagaagagaa ccagccagtg 2461 gtgtttaacc acgtttacaa catcaagctg ccagtgggat cccagtgttc ggtggatctg 2521 gagtcagcca gtggggagaa agacctggca ccgccttcag agcccagcga aagctttcag 2581 gagcacacag tggatgggga aaaccagatt gtcttcacac atcgcatcaa catcccccgc 2641 cgggcctgtg gctgtgccgc agcccctgat gttaaggagc tgctgagcag actggaggag 2701 ctggagaacc tggtgtcttc cctgagggag caatgtactg caggagcagg ctgctgtctc 2761 cagcctgcca caggccgctt ggacaccagg cccttctgta gcggtcgggg caacttcagc 2821 actgaaggat gtggctgtgt ctgcgaacct ggctggaaag gccccaactg ctctgagccc 2881 gaatgtccag gcaactgtca ccttcgaggc cggtgcattg atgggcagtg catctgtgac 2941 gacggcttca cgggcgagga ctgcagccag ctggcttgcc ccagcgactg caatgaccag 3001 ggcaagtgcg taaatggagt ctgcatctgt ttcgaaggct acgccggggc tgactgcagc 3061 cgtgaaatct gcccagtgcc ctgcagtgag gagcacggca catgtgtaga tggcttgtgt 3121 gtgtgccacg atggctttgc aggcgatgac tgcaacaagc ctctgtgtct caacaattgc 3181 tacaaccgtg gacgatgcgt ggagaatgag tgcgtgtgtg atgagggttt cacgggcgaa 3241 gactgcagtg agctcatctg ccccaatgac tgcttcgacc ggggccgctg catcaatggc 3301 acctgctact gcgaagaagg cttcacaggt gaagactgcg ggaaacccac ctgcccacat 3361 gcctgccaca cccagggccg gtgtgaggag gggcagtgtg tatgtgatga gggctttgcc 3421 ggtgtggact gcagcgagaa gaggtgtcct gctgactgtc acaatcgtgg ccgctgtgta 3481 gacgggcggt gtgagtgtga tgatggtttc actggagctg actgtgggga gctcaagtgt 3541 cccaatggct gcagtggcca tggccgctgt gtcaatgggc agtgtgtgtg tgatgagggc 3601 tatactgggg aggactgcag ccagctacgg tgccccaatg actgtcacag tcggggccgc 3661 tgtgtcgagg gcaaatgtgt atgtgagcaa ggcttcaagg gctatgactg cagtgacatg 3721 agctgcccta atgactgtca ccagcacggc cgctgtgtga atggcatgtg tgtttgtgat 3781 gacggctaca caggggaaga ctgccgggat cgccaatgcc ccagggactg cagcaacagg 3841 ggcctctgtg tggacggaca gtgcgtctgt gaggacggct tcaccggccc tgactgtgca 3901 gaactctcct gtccaaatga ctgccatggc cagggtcgct gtgtgaatgg gcagtgcgtg 3961 tgccatgaag gatttatggg caaagactgc aaggagcaaa gatgtcccag tgactgtcat 4021 ggccagggcc gctgcgtgga cggccagtgc atctgccacg agggcttcac aggcctggac 4081 tgtggccagc actcctgccc cagtgactgc aacaacttag gacaatgcgt ctcgggccgc 4141 tgcatctgca acgagggcta cagcggagaa gactgctcag aggtgtctcc tcccaaagac 4201 ctcgttgtga cagaagtgac ggaagagacg gtcaacctgg cctgggacaa tgagatgcgg 4261 gtcacagagt accttgtcgt gtacacgccc acccacgagg gtggtctgga aatgcagttc 4321 cgtgtgcctg gggaccagac gtccaccatc atccaggagc tggagcctgg tgtggagtac 4381 tttatccgtg tatttgccat cctggagaac aagaagagca ttcctgtcag cgccagggtg 4441 gccacgtact tacctgcacc tgaaggcctg aaattcaagt ccatcaagga gacatctgtg 4501 gaagtggagt gggatcctct agacattgct tttgaaacct gggagatcat cttccggaat 4561 atgaataaag aagatgaggg agagatcacc aaaagcctga ggaggccaga gacctcttac 4621 cggcaaactg gtctagctcc tgggcaagag tatgagatat ctctgcacat agtgaaaaac 4681 aatacccggg gccctggcct gaagagggtg accaccacac gcttggatgc ccccagccag 4741 atcgaggtga aagatgtcac agacaccact gccttgatca cctggttcaa gcccctggct 4801 gagatcgatg gcattgagct gacctacggc atcaaagacg tgccaggaga ccgtaccacc 4861 atcgatctca cagaggacga gaaccagtac tccatcggga acctgaagcc tgacactgag 4921 tacgaggtgt ccctcatctc ccgcagaggt gacatgtcaa gcaacccagc caaagagacc 4981 ttcacaacag gcctcgatgc tcccaggaat cttcgacgtg tttcccagac agataacagc 5041 atcaccctgg aatggaggaa tggcaaggca gctattgaca gttacagaat taagtatgcc 5101 cccatctctg gaggggacca cgctgaggtt gatgttccaa agagccaaca agccacaacc 5161 aaaaccacac tcacaggtct gaggccggga actgaatatg ggattggagt ttctgctgtg 5221 aaggaagaca aggagagcaa tccagcgacc atcaacgcag ccacagagtt ggacacgccc 5281 aaggaccttc aggtttctga aactgcagag accagcctga ccctgctctg gaagacaccg 5341 ttggccaaat ttgaccgcta ccgcctcaat tacagtctcc ccacaggcca gtgggtggga 5401 gtgcagcttc caagaaacac cacttcctat gtcctgagag gcctggaacc aggacaggag 5461 tacaatgtcc tcctgacagc cgagaaaggc agacacaaga gcaagcccgc acgtgtgaag 5521 gcatccactg aacaagcccc tgagctggaa aacctcaccg tgactgaggt tggctgggat 5581 ggcctcagac tcaactggac cgcagctgac caggcctatg agcactttat cattcaggtg 5641 caggaggcca acaaggtgga ggcagctcgg aacctcaccg tgcctggcag ccttcgggct 5701 gtggacatac cgggcctcaa ggctgctacg ccttatacag tctccatcta tggggtgatc 5761 cagggctata gaacaccagt gctctctgct gaggcctcca caggggaaac tcccaatttg 5821 ggagaggtcg tggtggccga ggtgggctgg gatgccctca aactcaactg gactgctcca 5881 gaaggggcct atgagtactt tttcattcag gtgcaggagg ctgacacagt agaggcagcc 5941 cagaacctca ccgtcccagg aggactgagg tccacagacc tgcctgggct caaagcagcc 6001 actcattata ccatcaccat ccgcggggtc actcaggact tcagcacaac ccctctctct 6061 gttgaagtct tgacagagga tctcccacag ctgggagatt tagccgtgtc tgaggttggc 6121 tgggatggcc tcagactcaa ctggaccgca gctgacaatg cctatgagca ctttgtcatt 6181 caggtgcagg aggtcaacaa agtggaggca gcccagaacc tcacgttgcc tggcagcctc 6241 agggctgtgg acatcccggg cctcgaggct gccacgcctt atagagtctc catctatggg 6301 gtgatccggg gctatagaac accagtactc tctgctgagg cctccacagc caaagaacct 6361 gaaattggaa acttaaatgt ttctgacata actcccgaga gcttcaatct ctcctggatg 6421 gctaccgatg ggatcttcga gacctttacc attgaaatta ttgattccaa taggttgctg 6481 gagactgtgg aatataatat ctctggtgct gaacgaactg cccatatctc agggctaccc 6541 cctagtactg attttattgt ctacctctct ggacttgctc ccagcatccg gaccaaaacc 6601 atcagtgcca cagccacgac agaagccgaa ccggaagttg acaaccttct ggtttcagat 6661 gccaccccag acggtttccg tctgtcctgg acagctgatg aaggggtctt cgacaatttt 6721 gttctcaaaa tcagagatac caaaaagcag tctgagccac tggaaataac cctacttgcc 6781 cccgaacgta ccagggacat aacaggtctc agagaggcta ctgaatacga aattgaactc 6841 tatggaataa gcaaaggaag gcgatcccag acagtcagtg ctatagcaac aacagccatg 6901 ggctccccaa aggaagtcat tttctcagac atcactgaaa attcggctac tgtcagctgg 6961 agggcaccca cagcccaagt ggagagcttc cggattacct atgtgcccat tacaggaggt 7021 acaccctcca tggtaactgt ggacggaacc aagactcaga ccaggctggt gaaactcata 7081 cctggcgtgg agtaccttgt cagcatcatc gccatgaagg gctttgagga aagtgaacct 7141 gtctcagggt cattcaccac agctctggat ggcccatctg gcctggtgac agccaacatc 7201 actgactcag aagccttggc caggtggcag ccagccattg ccactgtgga cagttatgtc 7261 atctcctaca caggcgagaa agtgccagaa attacacgca cggtgtccgg gaacacagtg 7321 gagtatgctc tgaccgacct cgagcctgcc acggaataca cactgagaat ctttgcagag 7381 aaagggcccc agaagagctc aaccatcact gccaagttca caacagacct cgattctcca 7441 agagacttga ctgctactga ggttcagtcg gaaactgccc tccttacctg gcgacccccc 7501 cgggcatcag tcaccggtta cctgctggtc tatgaatcag tggatggcac agtcaaggaa 7561 gtcattgtgg gtccagatac cacctcctac agcctggcag acctgagccc atccacccac 7621 tacacagcca agatccaggc actcaatggg cccctgagga gcaatatgat ccagaccatc 7681 ttcaccacaa ttggactcct gtaccccttc cccaaggact gctcccaagc aatgctgaat 7741 ggagacacga cctctggcct ctacaccatt tatctgaatg gtgataaggc tgaggcgctg 7801 gaagtcttct gtgacatgac ctctgatggg ggtggatgga ttgtgttcct gagacgcaaa 7861 aacggacgcg agaacttcta ccaaaactgg aaggcatatg ctgctggatt tggggaccgc 7921 agagaagaat tctggcttgg gctggacaac ctgaacaaaa tcacagccca ggggcagtac 7981 gagctccggg tggacctgcg ggaccatggg gagacagcct ttgctgtcta tgacaagttc 8041 agcgtgggag atgccaagac tcgctacaag ctgaaggtgg aggggtacag tgggacagca 8101 ggtgactcca tggcctacca caatggcaga tccttctcca cctttgacaa ggacacagat 8161 tcagccatca ccaactgtgc tctgtcctac aaaggggctt tctggtacag gaactgtcac 8221 cgtgtcaacc tgatggggag atatggggac aataaccaca gtcagggcgt taactggttc 8281 cactggaagg gccacgaaca ctcaatccag tttgctgaga tgaagctgag accaagcaac 8341 ttcagaaatc ttgaaggcag gcgcaaacgg gcaggatccG ACTACAAAGA CCATGACGGT 8401 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 8461 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 8521 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 8581 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 8641 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 8701 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 8761 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 8821 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 8881 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 8941 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 9001 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 9061 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 9121 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 9181 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 9241 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 9301 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 9361 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 9421 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 9481 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 9541 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 9601 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 9661 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 9721 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 9781 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 9841 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 9901 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 9961 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 10021 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 10081 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 10141 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 10201 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 10261 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 10321 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 10381 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 10441 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 10501 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 10561 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 10621 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 10681 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 10741 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 10801 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 10861 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 10921 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 10981 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 11041 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 11101 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 11161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 11221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 11281 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 11341 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 11401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 11461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 11521 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 11581 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 11641 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 11701 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 11761 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 11821 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 11881 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 11941 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 12001 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 12061 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 12121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 12181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 12241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 12301 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 12361 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 12421 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 12481 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 12541 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 12601 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 12661 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 12721 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 12781 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 12841 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 12901 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 12961 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 13021 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 13081 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 13141 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 13201 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 13261 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 13321 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 13381 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 13441 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 13501 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 13561 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 13621 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 13681 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 13741 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 13801 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 13861 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 13921 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 13981 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 14041 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 14101 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 14161 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 14221 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 14281 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 14341 caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.